Optical fiber living body fluorescence excitation spectral imaging device

Abstract

The invention relates to an optical fiber living body fluorescent excitation spectral imaging device which comprises interference modulated light source unit, a microscopic imaging unit, an optical fiber bundle endoscopic unit and a computer, wherein light of the interference modulated light source unit is coupled into the optical fiber bundle endoscopic unit through the microscopic imaging unit, the light output by the optical fiber bundle endoscopic unit irradiates on a sample, the sample emits a fluorescent signal after being irradiated, the fluorescent signal is collected by the optical fiber bundle endoscopic unit and returns and enters the microscopic imaging unit, one end port of the microscopic imaging unit is connected with the computer, and the computer is used for processing the electric signal of the microscopic imaging unit so as to obtain an objective fluorescence excitation spectral imaging device. The optical fiber body fluorescence excitation spectral imaging device has the advantages of being capable of not only providing the form distribution of a sample imaging space, but also providing accurate fluorescence excitation spectral imaging information of each point of the imaging space, achieving simultaneous observation of different biochemical constituents of a biological sample self or varieties of exogenous fluorescent marker excitation spectrums, and providing a novel experimental instrument for the visualization of functional studies of living cells.

Description

technical field [0001] The technical field of optical imaging of the present invention, in particular, relates to a fiber optic living body fluorescence excitation spectrum imaging device. Background technique [0002] The changes in cell function are involved in various physiological and pathological processes of the life cycle of cells. Confirming the true function of cells under living conditions is the key to uncovering the mysteries of life and conquering diseases from the cellular level. Therefore, in vivo detection and study of cell function changes under different physiological and pathological conditions is a major research direction in the field of biomedicine, and has become a hot spot in biomedical research in recent years. Fluorescence spectral microscopy imaging technology can detect multi-cellular events related to cell function in parallel, and is one of the important technical means for the visualization of living cell function research. However, the study ...

AI Technical Summary

Problems solved by technology

Although the principles of the above methods are different, they all use the fluorescence emission spectrum as the spectral characteristic quantity to perform spectral imaging. Therefore, different components need to be added in front of the detector to realize the spectral detection of the weak fluorescence emission signal of the living tissue. Therefore, Reduced system signal-to-noise ratio

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