Coaxial type axial modulation high-resolution photoacoustic microscopic imaging method and imaging system

Abstract

The invention discloses a coaxial axial modulation high-resolution photoacoustic microscopic imaging method and an imaging system, belongs to the field of microscopic imaging, and particularly establishes a set of complete coaxial axial modulation high-resolution photoacoustic microscopic imaging system to avoid the problem of poor axial resolution in an orthogonal axial structure light modulation photoacoustic signal scheme. Meanwhile, the invention further provides a set of axial modulation photoacoustic signal method, the axial structure light modulation principle is combined with ultrasonic detection, the limitation that the axial resolution of the PAM system is limited by the detection bandwidth of an ultrasonic probe is broken through, the axial high-resolution PAM system imaging result is obtained, and meanwhile, the defect that light damage exists when the axial resolution of an existing PAM system is improved is overcome. In the method for axially modulating the photoacoustic signal, a coaxial axial structured light implementation scheme is provided on the basis of a double circular seam method, the three-dimensional light field distribution of circular seam masks with different initial phase differences near the rear focal plane of the lens can be easily obtained by changing an input light field, and the calculation is simple.

Description

technical field [0001] The invention relates to the field of microscopic imaging, in particular to a coaxial axial modulation high-resolution photoacoustic microscopic imaging method and an imaging system. Background technique [0002] The microcirculatory system is a key place for material exchange between blood and interstitial fluid, and the maintenance of its structure and function is a necessary condition for the normal work of tissues. Microcirculatory system disorders often affect blood flow rate or form thrombus, leading to local tissue ischemia or even necrosis, causing a series of clinical symptoms. Various imaging techniques have been developed for imaging vascular networks. However, these techniques cannot simultaneously meet the imaging needs of the microcirculatory system. Photoacoustic Microscopy (PAM) is more suitable for observing the structure and function of microcirculatory system due to its advantages of high optical absorption contrast and high acoust...

AI Technical Summary

Problems solved by technology

First of all, although the system can improve the axial resolution, in this orthogonal confocal system, the lateral resolution of the system is determined by the axial resolution of the objective lens and the lateral resolution of the UST, which deteriorates the lateral resolution of the system and cannot Obtain 3D imaging results with high spatial resolution

Secondly, the system can only modulate samples that are on the focal plane of the objective lens. For samples that deviate from the focal plane of the objective lens, the structured light modulation effect will drop sharply as the structured light defocuses.

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