The invention provides an establishing method of double-
solvent fused
HPLC fingerprint of medicinal
phellodendron. The method comprises the following steps: (1) preparing a reference solution: preparing a
berberine hydrochloride reference solution; (2) preparing test solutions: weighing medicinal
phellodendron or cortex phellodendri chinensis, extracting the weighed medicinal
phellodendron or cortex phellodendri chinensis with two solvents, wherein the two solvents are representative and complementary, filtering the extracting solutions with a micro-porous filter membrane so as to obtain the test solutions; (3) measuring the obtained test solutions through a
high performance liquid chromatography (HPLC) method so as to obtain fingerprints, wherein the
chromatography conditions are as follows:
chromatographic column is filled with octadecylsilane chemically-bonded silica,
gradient elution is adopted, and the
ultraviolet detection
wavelength is 300 nm; (4) fusing the two fingerprints of parts respectively extracted by two solvents so as to establish a double-
solvent fused
fingerprint of medicinal phellodendron. The test
fingerprint is evaluated by the "Similarity
Evaluation System for
Chromatographic Fingerprint of
Traditional Chinese Medicine" published by
Chinese Pharmacopoeia Commission (Version 2004A). The establish
fingerprint has rich fingerprint characteristic information and the characteristics of simple method, good
repeatability, and reliability, is capable of identifying cortex phellodendri chinensis, medicinal phellodendron and counterfeit, and provides a reference for identification and overall
quality control of medicinal phellodendron.