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Method for establishing Tripterygium wilfordii fingerprint chromatography and standard fingerprint chromatography thereof

A standard fingerprint and fingerprint technology, applied in the field of drug analysis, can solve the problems of lack of standard control methods for Tripterygium wilfordii medicinal materials, and achieve the effects of easy realization of chromatographic conditions, simple production, good stability and reproducibility

Inactive Publication Date: 2010-02-10
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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AI Technical Summary

Problems solved by technology

[0006] At present, the quality evaluation of Tripterygium wilfordii mainly focuses on the qualitative and quantitative analysis of an active component or an index component, and there is a lack of standard control methods to fully reflect the quality of Tripterygium wilfordii

Method used

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  • Method for establishing Tripterygium wilfordii fingerprint chromatography and standard fingerprint chromatography thereof

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Experimental program
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Embodiment Construction

[0018] Preferred embodiments of the present invention are as follows:

[0019] (1) Instruments and drugs: Shimadzu LC10A high-performance liquid chromatography was used; Tripterygium wilfordii medicinal materials were purchased in 17 batches from Chinese herbal medicine stores; other reagents were chromatographically pure or analytically pure.

[0020] (2) Preparation of the test solution: Accurately weigh 10 g of the dried medicinal material powder, place it in a stoppered container, add 80-120 mL of 60-80% methanol, oscillate ultrasonically for 60 min, transfer it into a Soxhlet extractor, and extract it under reflux 5h, the extract was concentrated to less than 10mL, filtered through a 0.45μm microporous membrane, and the filtrate was dilute to 10mL as the test solution.

[0021] (3) HPLC analysis: the chromatographic separation column packing is 5μm Kromasil-C18, 250mm×4.6mm; the mobile phase is water (0.4% phosphoric acid + 0.5% acetic acid)-acetonitrile; gradient elution...

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Abstract

The invention provides a method for establishing high performance liquid chromatography (HPLC) fingerprint chromatography of Tripterygium wilfordii and a standard fingerprint chromatography thereof, and the method comprises the steps of preparing test solution, selecting chromatographic conditions, manufacturing HPLC fingerprint chromatography, determining the standard fingerprint chromatography and the like. By comparing the HPLC Tripterygium wilfordii fingerprint chromatography in 17 batches to determine 24 common characteristic peaks, the common characteristic peaks form the fingerprint characteristics of the Tripterygium wilfordii which can be taken as the standard fingerprint chromatography of the Tripterygium wilfordii. The invention has the advantages of simple method, good repeatability, a lot of characteristic peaks, accuracy and reliability and the like. The established standard fingerprint chromatography can effectively represent quality of the Tripterygium wilfordii.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of Chinese medicinal materials, in particular to a method for establishing high-performance liquid chromatography (HPLC) fingerprints of Tripterygium wilfordii medicinal materials, and the HPLC standard fingerprints of tripterygium wilfordii medicinal materials obtained by the method. The invention belongs to the technical field of pharmaceutical analysis. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. At present, fingerprints have become one of the most effective means to identify the varieties of traditional Chinese medicines and evaluate the quality of traditional Chinese medicines recognized at home and abroad. In the "FDA ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/37A61P37/02A61P35/00A61P9/00A61P29/00A61P31/04A61P31/06A61P19/04A61P11/00G01N30/02G01N30/36A61K125/00A61K127/00A61K131/00A61K133/00
Inventor 周考文王树聪李凤桐
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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