The invention provides an establishment method for a high performance liquid chromatography (HPLC) fingerprint of a Rubia cordifolia root formula granule, and a Rubia cordifolia root formula granule standard fingerprint obtained thereby. The establishment method provided by the invention includes the steps of: (1) preparation of a test solution; and (2) high performance liquid chromatography analysis. The high performance liquid chromatography standard fingerprint of the Rubia cordifolia root formula granule aqueous solution obtained by the method has a total of 11 chromatographic peaks, the retention time of which is 6.072min, 6.559min, 8.087min, 9.170min, 12.118min, 20.988min, 29.251min, 32.211min, 33.189min, 38.430min and 45.431min respectively. The HPLC standard fingerprint of the Rubia cordifolia root formula granule fat-soluble components has a total of 9 chromatographic peaks, the retention times of which is 12.156min, 12.832min, 14.552min, 22.615min, 23.291min, 25.412min, 27.356min, 34.383min and 37.076min respectively. According to the invention, the test solution is simple to prepare, the chromatographic conditions are easy to realize, and the method has good stability and reproducibility, can effectively control the quality of the Rubia cordifolia root formula granule, and provides reference for quality control of the Rubia cordifolia root formula granule.