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Establishment method of scorzonera fingerprint spectrum and scorzonera standard fingerprint spectrum

A technology for fingerprinting and establishing methods, applied in the field of traditional Chinese medicine analysis, to achieve the effects of easy implementation, short test period and low column pressure

Inactive Publication Date: 2014-07-02
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, so far, there is no report about the fingerprints of the medicinal materials of Scallops

Method used

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  • Establishment method of scorzonera fingerprint spectrum and scorzonera standard fingerprint spectrum
  • Establishment method of scorzonera fingerprint spectrum and scorzonera standard fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the establishment of the fingerprint spectrum of Scallops

[0038] 1) Drugs, reagents and instruments

[0039] There are a total of 12 batches of Scallops medicinal materials, all collected in Yitong, Jilin Province; using Shimadzu LC-10AT VP Plus high-performance liquid chromatography; reagents are chromatographically pure or analytically pure;

[0040] 2) Preparation of the test solution

[0041] Precisely weigh 2.00g of the coarse powder of the coriander onion, put it in a container with a stopper, add 50ml of 70% ethanol precisely, weigh it after the stopper, extract it ultrasonically for 1 hour, let it stand still, let it cool down, make up the missing weight with 70% ethanol, and shake well , filtered. Take an appropriate amount of filtrate and filter it with a 0.45 μm microporous membrane as the test solution;

[0042] 3) Preparation of reference solution

[0043] Take Scutellaria flavone glycoside A (5,7,3′,4′-tetrahydroxyflavone-6-C-β-D-glucop...

Embodiment 2

[0047] Embodiment 2: Scallion standard fingerprint spectrum

[0048] There are a total of 12 batches of Yaonian medicinal materials, all of which were collected in Yitong, Jilin Province. The method described in the embodiment of the present invention 1 obtains the fingerprints of various batches of Scallops, uses the "Chinese Medicine Chromatography Fingerprint Similarity Evaluation System 2004 Edition" software analysis of the National Pharmacopoeia Commission, obtains the standard fingerprints of Scallops, and determines that 13 common Characteristic peaks, see figure 1 .

[0049] The 7th and 10th chromatographic peaks are corresponding to Scutellaria flavone glycoside A (5,7,3′,4′-tetrahydroxyflavone-6-C-β-D-glucopyranone glycoside) and Scutellaria glucoside D (5,7,4′-Trihydroxyflavone-8-C-β-D-glucopyranoside), the total length of the spectrum is 75min, the average retention time of peak No. 7 is 31.65min, with peak No. 7 as the reference peak , calculate the relative r...

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Abstract

The invention provides an establishment method of a scorzonera fingerprint spectrum. The establishment method comprises the following specific steps: preparing a test solution by scorzonera, and carrying out fractionation detection under a certain condition by a high performance liquid chromatograph to obtain the scorzonera fingerprint spectrum. The invention further provides a scorzonera standard fingerprint spectrum obtained by the method. The standard fingerprint spectrum can provide reliable basis for scorzonera identification and internal mass control. The establishment method is simple and convenient to operate, high in stability, good in reproducibility and multiple in characteristic peaks. By utilizing the establishment method, the quality of scorzonera medicinal materials can be comprehensively and accurately evaluated.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of jalapenos, and a standard fingerprint of jasmines obtained by the method, which belongs to the field of traditional Chinese medicine analysis. Background technique [0002] Scutellaria is the dried whole herb of Compositae plant Scorzonera austriaca Wild. Excavated and sun-dried from May to June. The total length of this product is 10-40 cm; the root is stout, obconic, about 0.3-3 cm in diameter; the stems are numerous, clustered at the top of the root neck, unbranched, smooth and hairless, and the stem base is torn by dense brown fibrous Sheath-like remnants; basal leaves broadly lanceolate to oblong-ovate, 20-30(-40) cm long, 1.2-3.5(-5) cm wide, with flat margins, sheath-like expansion at the base, sheath-like sheath Dense cotton wool, with cotton wool on the edge of the base; a few cauline leaves, 1 to 3, scale-like; flower heads solitary on the top of the stem, achenes glabrous. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 王广树张爽谢扬周小平孙薇王艳杨锦竹张沐新刘银燕
Owner JILIN UNIV
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