Chinese angelica standard decoction fingerprint spectrum, characteristic spectrum establishment method and content determination method

A method for establishing a fingerprint, a technique for establishing a fingerprint, applied in the establishment of a characteristic spectrum and content determination, and in the field of the fingerprint of an angelica standard decoction, achieving the effect of saving material and time costs, stable and reliable quality standards, and good separation

Inactive Publication Date: 2019-07-30
SINOPHARM GRP DEZHONG (FOSHAN) PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the technical field of analysis of Angelica medicinal materials, ultra-high-speed liquid chromatography (UPLC) has not yet been used to carry out quality control analysis of Angelica standard decoction and report its fingerprint.

Method used

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  • Chinese angelica standard decoction fingerprint spectrum, characteristic spectrum establishment method and content determination method
  • Chinese angelica standard decoction fingerprint spectrum, characteristic spectrum establishment method and content determination method
  • Chinese angelica standard decoction fingerprint spectrum, characteristic spectrum establishment method and content determination method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0063] Preparation of Angelica Standard Decoction:

[0064] Take 150g of angelica decoction pieces, add water to decoct twice, add 9 times the amount of water to the first decoction, soak for 40 minutes, decoct for 60 minutes, filter while hot with a 400-mesh sieve, and quickly cool the filtrate with cold water. Add 7 times the amount of water for the second decoction, decoct for 40 minutes, filter while it is hot with a 400-mesh sieve, cool the filtrate quickly with cold water, and combine the two filtrates. Transfer the combined filtrate to a rotary evaporator to concentrate under reduced pressure, concentrate to an appropriate amount, and freeze-dry to obtain the angelica standard decoction.

Embodiment 1

[0065] Embodiment 1: Fingerprint is established

[0066] 1. Investigation on Chromatographic Conditions of Angelica Standard Decoction

[0067] 1. Chromatographic conditions and system suitability test

[0068] Accurately draw 5 μl of the test solution respectively, inject it into a liquid chromatograph, measure, record the chromatogram, and obtain it. Table 1 shows the initial conditions of the fingerprint of Angelica Standard Decoction, and Table 2 shows the mobile phase gradient table. Determine according to high performance liquid chromatography ("Chinese Pharmacopoeia" 2015 edition four general rules 0512).

[0069] Table 1 The initial conditions of the fingerprints of Angelica standard decoction

[0070]

[0071]

[0072] Table 2 Initial mobile phase gradient table

[0073] time (minutes) Mobile phase A(%) Mobile phase B(%) 0 10 90 7 90 10 10 100 0

[0074] (1) Selection of detection wavelength

[0075] On the basis of the ...

Embodiment 2

[0142] The establishment of embodiment 2 feature map

[0143] Chromatographic conditions: according to the final experimental conditions proposed in Example 1.

[0144] Preparation of the reference substance solution: take an appropriate amount of the reference substance of ferulic acid, weigh it accurately, put it in a brown measuring bottle, add methanol to make a solution containing 12 μg per 1 ml, and obtain it.

[0145] Determination method: Precisely draw 1 μl of the reference substance solution and 5 μl of the test product solution respectively, inject it into the liquid chromatograph, measure, record the chromatogram, and obtain it.

[0146] One, the establishment of the method for preparing the test solution

[0147] 1. Selection of extraction solvent

[0148] Take about 0.15g of this product (batch number B01), weigh it precisely, put it into a stoppered Erlenmeyer flask, add methanol, 50% methanol, and 25ml of water precisely, shake well, weigh, ultrasonicate for ...

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Abstract

The invention discloses a Chinese angelica standard decoction fingerprint spectrum, a characteristic spectrum establishment method and a content determination method. The establishment method for thefingerprint spectrum comprises the following steps of 1) preparing a test solution; 2) carrying out chromatographic condition and system suitability test; and 3) injecting the test solution into a liquid chromatograph, and measuring to obtain a fingerprint spectrum. An ultra-high performance liquid chromatography fingerprint spectrum of the Chinese angelica standard decoction is researched, so that a better Chinese angelica standard decoction fingerprint spectrum is obtained, and a novel technical method is provided for controlling the quality of the preparation.

Description

technical field [0001] The invention relates to the field of drug analysis, and mainly relates to a method for establishing a fingerprint and a characteristic map of an angelica standard decoction and determining its content. Background technique [0002] Decoction is one of the earliest and most widely used dosage forms in traditional Chinese medicine clinics in my country, and it is the most important dosage form for patients to take traditional Chinese medicine. Under the background of uneven quality and diversified forms of decoction pieces of traditional Chinese medicine, the quality of decoction is the most direct way to evaluate the quality of decoction pieces and even the curative effect of traditional Chinese medicine. The standard decoction of Chinese herbal decoction pieces is a decoction of single-flavored decoction pieces prepared by a standardized process based on the theory of traditional Chinese medicine and clinical application, referring to modern extractio...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8631G01N30/8686
Inventor 相雨杜蔼媚卢国扬马存
Owner SINOPHARM GRP DEZHONG (FOSHAN) PHARM CO LTD
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