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Establishing method of double-solvent fused HPLC fingerprint of medicinal phellodendron and standard fingerprint of medicinal phellodendron

A technique of fingerprinting and establishing methods, which is applied in the field of pharmaceutical analysis, can solve the problems of scattered identification and complex methods, and achieve the effects of short analysis time, easy chromatographic conditions, and simple production

Inactive Publication Date: 2014-06-18
DIHON PHARMA GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the identification of Phellodendron Phellodendron, Cortex Phellodendron and its confounds in the literature is relatively scattered, and the methods are more complicated. There is no method that can directly and simply distinguish the varieties of Phellodendron Phellodendri

Method used

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  • Establishing method of double-solvent fused HPLC fingerprint of medicinal phellodendron and standard fingerprint of medicinal phellodendron
  • Establishing method of double-solvent fused HPLC fingerprint of medicinal phellodendron and standard fingerprint of medicinal phellodendron
  • Establishing method of double-solvent fused HPLC fingerprint of medicinal phellodendron and standard fingerprint of medicinal phellodendron

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1: the detection of the HPLC fingerprint of Phellodendron Phellodendron produced in Sichuan

[0039] 1 Experimental materials and instruments

[0040] 1.1 Medicinal materials: Phellodendron Phellodendron produced in Sichuan has a total of 10 batches, which are respectively recorded as (HB-1~HB-10).

[0041] 1.2 Instruments: DIONEX Ultimate3000 high performance liquid chromatography (quaternary gradient pump; column thermostat; DAD detector; autosampler; Chromeleon chromatography data system); METTLER TOLEDO AL204-S electronic balance; METTLER TOLEDO PH meter; KQ- 250DE medical numerical control ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.; LXJ-II B low-speed and large-capacity multi-tube centrifuge (Shanghai Anting Scientific Instrument Factory);

[0042] 1.3 Reagents: standard berberine hydrochloride (National Institute for the Control of Pharmaceutical and Biological Products, batch number: 110713-200606), acetonitrile is chromatographically...

Embodiment 2

[0050] Embodiment 2: identification of Cortex Phellodendri medicinal material and Cortex Phellodendri, confounding product

[0051] 1 Experimental materials and instruments

[0052] 1.1 Medicinal materials: There are 5 batches of Phellodendron contaminates, which are recorded as (HB-11~HB-15), and Phellodendron phellodendri in Shanguan, Liaoning Province is recorded as HB-L.

[0053] 1.2 Instruments: DIONEX Ultimate3000 high performance liquid chromatography (quaternary gradient pump; column thermostat; DAD detector; automatic sampler; Chromeleon chromatography data system); METTLERTOLEDO AL204-S electronic balance; METTLER TOLEDO PH meter; KQ-250DE Type medical numerical control ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.; LXJ-II B type low-speed and large-capacity multi-tube centrifuge (Shanghai Anting Scientific Instrument Factory);

[0054] 1.3 Reagents: standard berberine hydrochloride (National Institute for the Control of Pharmaceutical and Biological P...

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Abstract

The invention provides an establishing method of double-solvent fused HPLC fingerprint of medicinal phellodendron. The method comprises the following steps: (1) preparing a reference solution: preparing a berberine hydrochloride reference solution; (2) preparing test solutions: weighing medicinal phellodendron or cortex phellodendri chinensis, extracting the weighed medicinal phellodendron or cortex phellodendri chinensis with two solvents, wherein the two solvents are representative and complementary, filtering the extracting solutions with a micro-porous filter membrane so as to obtain the test solutions; (3) measuring the obtained test solutions through a high performance liquid chromatography (HPLC) method so as to obtain fingerprints, wherein the chromatography conditions are as follows: chromatographic column is filled with octadecylsilane chemically-bonded silica, gradient elution is adopted, and the ultraviolet detection wavelength is 300 nm; (4) fusing the two fingerprints of parts respectively extracted by two solvents so as to establish a double-solvent fused fingerprint of medicinal phellodendron. The test fingerprint is evaluated by the "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine" published by Chinese Pharmacopoeia Commission (Version 2004A). The establish fingerprint has rich fingerprint characteristic information and the characteristics of simple method, good repeatability, and reliability, is capable of identifying cortex phellodendri chinensis, medicinal phellodendron and counterfeit, and provides a reference for identification and overall quality control of medicinal phellodendron.

Description

technical field [0001] The invention relates to a method for constructing fingerprints of traditional Chinese medicinal materials, in particular to a quality control method for dual-solvent fusion fingerprints of Phellodendron phellodendron. The invention belongs to the technical field of pharmaceutical analysis. Background technique [0002] Phellodendron chinensis (PHELLODENDRI CHINENSIS CORTEX) is the dry bark of Rutaceae Phellodendron chinense Schneid., commonly known as "Sichuan Phellodendron" (National Pharmacopoeia Commission: Chinese Pharmacopoeia, Part One. Beijing, Chemical Industry Press, 2010: 153, 286), is a commonly used traditional Chinese medicine, which has the functions of clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores, and is widely used in clinical practice. Bitter in the mouth, cold, returns kidney, bladder, large intestine meridian. Efficacy clearing heat and dampness, purging fire and detoxification, ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 杨义芳黄春跃唐博雅罗泽渊李存勇朱绪民蒲琴
Owner DIHON PHARMA GROUP
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