45 results about "Trichomonascus" patented technology

The invention aims to provide a combined detection kit for vaginitis, capable of evaluating vaginal micro-ecological environment, vaginal cleanliness, and pathogens of bacterial vaginosis, Candida vaginitis, trichomonas vaginitis of the tested person simultaneously. Ihe following technical scheme adopted by the invention, a combined detection kit for vaginitis is provided and comprises a reaction device, diluent and chromogenic liquid, the reaction device is a hole type box-shaped reaction device with six reacting blind holes, in which a hydrogen peroxide concentration detection reagent pad, a leukocyte esterase detection reagent pad, a neuraminidase detection reagent pad, a proline aminopeptidase detection reagent pad, an acetyl glucosaminidase detection reagent pad and pH detection test paper are arranged respectively. Through the combined detection, the vaginitis caused by most of the BV (Bacterial Vaginosis) pathogens, the Candida albicans and the trichomonas can be detected, BV missing detection caused by the simple detection for one kind of enzyme activity is avoided largely, and the detection reliability and accuracy are increased.

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need oftwo main, and its detection accuracy rate is high, can be up to 98%.

The invention discloses a medicine for treating mycotic vaginitis, which is prepared from the following raw materials in part by weight: 35 to 45 parts of phellodendron, 30 to 40 parts of cortex dictamni, 30 to 40 parts of baical skullcap root, 40 to 60 parts of gentian, 30 to 40 parts of gardenia, 25 to 35 parts of bupleurum, 25 to 35 parts of glabrous greenbrier rhizome, 30 to 40 parts of largehead atractylodes rhizome, 15 to 25 parts of liquorice, 40 to 60 parts of giant knotweed rhizome, 30 to 40 parts of belvedere fruit, 25 to 35 parts of akebia stem, 40 to 60 parts of lightyellow sophora root, 30 to 40 parts of sessile stemona root, 25 to 35 parts of common cnidium fruit, 30 to 40 parts of atraotydin (fried) and 25 to 35 parts of weeping forsythia. Through compatibility of the medicines, the multiple effects of resisting and killing bacteria, resisting trichomonad, relieving itching, resisting tumors and the like are achieved, and the medicine is mainly used for clearing heat and removing dampness, inhibiting mycete and stopping leucorrhea, relieving vaginal itching, and dispelling the wind and killing the trichomonad, and has good effect on the mycotic vaginitis, trichomonas vaginitis, viral vaginitis and cervicitis; moreover, the medicine radically cures the diseases and ensures no relapse.

Proguanil has been found to have rapid and effective killing activity against a variety of disease-causing micro organisms. For example, when applied topically, proguanil is particularly effective against Propionibacterium acnes, a bacteria that causes acne; Corynebacterium minutissimum, a bacteria that causes erythrasma, Gardnerella vaginalis, a bacteria that causes vaginosis; Trichomonas vaginalis, a protozoan that causes trichomoniasis and C. albicans, a fungus (a form of yeast).

The present invention provides a Chinese medicine composition for external application for vagina. Said Chinese medicine composition is made up by using 7 Chinese medicinal materials of sophora alopecuroides, baeckea frutescens, phellodendron bark, violet, cnidium seed, borneol and licorice through a certain preparation process. Said invention has the effects of resisting bateria, relieving inflammation, resisting trichomonad and relieving itching, etc.

The present invention provides methods and compositions for identifying a subject at increased risk of having prostate cancer by detecting an antibody that specifically binds a Trichomonas α-actinin protein in a sample of the subject.

The invention relates to a method for preparing novel gynecological policresulen vaginal tablet used for antisepsis and anti-inflammation and a quality control method thereof. The policresulen has broad-spectrum antibacterial effect on common gram-positive bacterium, gram-negative bacterium, fungus and some viruses, wherein, anaerobe trichomonas and monilia are particularly sensitive to the policresulen but vaginal lactobacilli flora is hardly effaced. The invention has the advantages that vagina local medication avoids or reduces possible untoward effect caused by systemic administration; highly effective concentration of the medicine on lesion site is in favor of developing therapeutic effect, effectively protecting physiological environment of the vagina and providing suitable life condition for good bacteria; wherein, prescription of 1,000 preparation units of the medicine is: 45-180g of the policresulen, 30-180g of filler lactose, 60-500g of starch, 50-500g of kaolin, 2-10g of bonding agent hypromellose, 6-50g of fluidizer silicon dioxide, 30-300g of disintegrating agent microcrystalline cellulose, 20-200g of polyvinyl polypyrrolidone and 1-10g of lubricant magnesium stearate.

The present invention provides methods and compositions for detecting a Trichomonas antigen in a fixed sample and for diagnosing Trichomonas infection by detecting a Trichomonas antigen in a fixed sample.

The present disclosure provides agricultural compositions and methods of using these compositions to increase plant growth, pathogen resistance and drought tolerance. The agricultural compositions disclosed herein comprise mixtures of mutualistic beneficial fungi such as Laccaria bicolor and Piriformispora indica, and bacterial strains of Pseudomonas.

The present invention is Chinese medicine composition for treating women's diseases and its production process. The externally applied medicine is used in treating women's inflammation caused by trichomonas vaginalis, fungus and bacteria. The Chinese medicine composition contains honeysuckle, sessile stemona root, phellodendron bark and other plant medicine materials, borax and borneol.

A novel veterinary anti-inflammation agent and a preparation method of the same. The multifunctional veterinary anti-inflammation agent is prepared by extracting effective components from following traditional Chinese medicine raw materials including: pseudo-ginseng, gentiana scabra, radix stellariae, herba houttuyniae, rhubarb, sophora flavescens, dandelion, cowherb seeds and liquidambar formosana and the like, by means of a bio-fermentation engineering technology and a bio-chemical engineering technology according to a special process and a special formula. The anti-inflammation agent can safely, high-effectively, quickly and synchronously kill six categories of pathogenic microorganisms including bacteria, fungus, viruses, mycoplasma, chlamydia and protozoons (including staphylococcus aureus, escherichia coli, candida albicans, herpes virus vaginalis, mycoplasma urealytium, chlamydia vaginalis, trichomonas vaginalis and the like). The anti-bacterial range of the anti-inflammation agent greatly exceeds a limit that conventional antibiotics only can kill and inhibit bacteria. The anti-inflammation agent is free of generation of polluting substances not only in production but also in use, is low in production cost, is good in effects and is free of toxic and side effects, can be used for replacing the antibiotics to eliminate damage on human body and livestock due to "antibiotic-milk" and "antibiotic-meat", can effectively prevent and treat inflammation ingenital tract and mammary gland of cattle, horses, sheep, pigs, dogs, wild animals and pets, and is very wide in market prospect.

A method and kit for detecting Trichomonas vaginalis infection in a human subject are disclosed. In the method, a body-fluid sample such as a vaginal-swab sample or urine is obtained from the subject and contacted with an antibody specific against a Trichomonas adhesin peptide, forming an antibody-adhesin peptide complex if the subject is infected with Trichomonas. The presence or absence of the complex establishes, with a reliability of at least 80%, in the case of a vaginal swab sample, and with a reliability of at least 40% in the case of a urine sample, the presence or absence, respectively, of Trichomonas infection in the subject. A preferred test kit employs a dry-strip, sandwich assay, format.

The present invention is directed to the discovery of single nucleotide polymorphisms (SNPs) in the presence of metronidazole-resistant Trichomonas vaginalis. The presence of G76C, C213G, or C318A (SNP) in tvntr 4 or the presence of A238T, G427C, or T476C (SNP) in tvntr6 provides a reliable biomarker for the presence of metronidazole-resistant Trichomonas vaginalis. The present invention further provides reagents used for detecting the SNPs to screen subjects for metronidazole resistance in Trichomonas vaginalis.

The invention provides a pharmaceutical composition for vaginal external use, which is a preparation prepared from the following raw materials: Sophora sophora, Pinus pine, Phellodendron cork, Ding kudiding, Fructus Cnidii, Borneol and licorice. The invention also provides a preparation method of the pharmaceutical composition and its application in the preparation of antibacterial, anti-inflammatory, anti-trichomonas and antipruritic drugs for topical vaginal administration; experiments show that the drug of the present invention has definite curative effect, clear therapeutic range, and carries The invention is convenient, has a delicate medicinal taste, and provides quality control standards, so that the medicine of the invention is safe, effective, stable and controllable.

The invention discloses medicinal composition for treating gynecological vaginitis and a preparation method of the medicinal composition. The medicinal composition comprises the active components: a hawthorn kernel extract and a comfrey extract, has good bactericidal and insecticidal effects, and has a therapeutic effect on vaginitis caused by bacteria, fungi and trichomonas infection.

The invention provides a medicine composition for treating Trichomonas vaginalis and mycotic infection and a preparation method thereof, and belongs to the field of preparation technology of traditional Chinese medicine. The suppository is extracted and prepared by taking natural traditional Chinese medicines, namely peppers, kuh-seng, and root barks of shaggy-fruited dittany as raw material components. The medicine composition comprises the following various raw materials in proportion by weight: 40 portions of the peppers, 40 portions of the kuh-seng and 20 portions of the root barks of shaggy-fruited dittany. The suppository is prepared by using ethanol to soak medicines, reclaiming the ethanol, drying and crushing the medicines, and adding a fatty acid substrate. As proved by clinical tests and animal experimental research, the reagent has the function of obviously preventing and treating Trichomonas vaginalis and mycotic vaginitis, and has no obvious untoward reaction.

The invention discloses a joint detection kit for detecting and distinguishing Trichomonas and Candida, which comprises coagulation enzyme activity detection reagent, α-glucosidase activity detection reagent and pH detection reagent; the enzyme substrate used in the coagulation enzyme activity detection reagent The substance is a Gly-Arg dipeptide substrate containing a chromogen or a fluorescent group; the enzyme substrate used in the α-glucosidase activity detection reagent is an α-D-glucoside substrate containing a chromogen or a fluorescent group . In order to improve the color developing effect, it may also include a color developing solution. The invention has the advantages of relatively high sensitivity and specificity, significantly improved performance of reagents compared with the existing similar methods, and can meet the needs of clinical detection.

The invention discloses a stranguria-clearing and toxin-removing decoction. The stranguria-clearing and toxin-removing decoction comprises the following raw materials in parts by weight: 8-12 parts ofcortex phellodendri, 13-17 parts of Chinese pulsatilla root, 8-12 parts of bittersweet herb, 18-22 parts of European verbena herb, 8-12 parts of flos sophorae, 4-8 parts of common threewingnut root,8-12 parts of Chinese thorowax root, 8-12 parts of cortex acanthopanacis, 13-17 parts of dark plum fruit, 8-12 parts of rhubarb, 2-6 parts of boat-fruited sterculia seed, 8-12 parts of zedoary, 14-16parts of white mulberry root-bark, 9-11 parts of semen aesculi and 9-11 parts of nutgrass galingale rhizome. The secondary medicine formula of the stranguria-clearing and toxin-removing decoction is mainly used for treating general symptoms of vaginitis, trichomonad, bleeding, cervicitis, cervical erosion and cervical cancer, and the symptoms can be better in half a month until the cervical canceris taken; and the metastatic cervical cancer can be decocted with bistort rhizome for about two hours, or otherwise, the drug effect is affected.

A dripping fill of ornidazole for suppressing and killing amebas, trichomonad and anaerobic bacteria is prepared from ornidazole and medicinal carrier. Its preparing process is also disclosed.

The invention provides a robust medicinal composition for treating gynecological diseases, a robust medicinal suppository and a preparation method of the robust medicinal suppository, and belongs to the technical field of robust medicinal compositions. The robust medicinal composition comprises the following components in parts by weight: 15 to 25 parts of glabrous greenbrier rhizome, 15 to 20 parts of flemingia philippinensis, 8 to 12 parts of stephania sinica diels, 13 to 17 parts of black gedanite, 8 to 12 parts of almond, 8 to 12 parts of bitter apricot kernels, 15 to 25 parts of honey, 15to 20 parts of wild coriander, 8 to 12 parts of fructus gardeniae stamens, 13 to 17 parts of rhizoma atractylodis and 13 to 17 parts of Atractylodes macrocephala. The traditional Chinese medicine composition is rigorous in formula, various components are mutually beneficial, and the traditional Chinese medicine composition can be used for treating colpitis mycotica, trichomonas vaginitis, bacterial vaginitis, senile vaginitis, cervical erosion, endometritis and pelvic inflammation.

The invention belongs in the field of medicine and can be used in the treatment of chronic and asymptomatic trichomonas infections of the urogenital tract.The invention is consisted in development of new therapeutic substance of plant origin with minimal side effects for treatment of various forms of trichomonad infection.The new therapeutic substance, of plant origin, is an olive coloured paste. It is composed of a conifer green needle complex (CGNC) and has a coniferous smell and antiprotozoal activity against Trichomonas vaginalis (when tested in an in vitro model).At a concentration of 100 mg / ml CGNC has trichomonadocitic and trichomonadostatic properties. At 300 mg / ml it suppresses the growth of T. vaginalis and at 500 mg / ml it kills this organism.

The invention discloses a mode for forming a nano-silver coating film on a stem cell culture suction tube under the assistance of magnetron sputtering and chemical gradient sedimentation, which comprises the following steps that a, the stem cell culture suction tube is disinfected and is cleaned through high-frequency oscillation; b, the stem cell culture suction tube is placed in a steady state, a three-dimensional maximum diameter under a horizontal condition is removed, a silver target is customized, and the stem cell culture suction tube is placed into the silver target; c, bombarding is conducted on the target object silver target through electromagnetic sputtering, a copper wire of 860 turns with the diameter of 0.1 cm is wound on an outer target on the outer side of a magnetron sputtering vacuum chamber, the vacuum chamber is cleaned, the substrate material (the stem cell culture suction tube) is placed in an inner target, the vacuum chamber is sealed and starts to be vacuumized with the vacuum degree being 10 <-6 >; and d, inert gas is input into the vacuum chamber for filling. The stem cell culture suction tube formed by the invention has the effects of killing bacteria, fungi, mycoplasma, chlamydia, spirochete and even trichomonad in clinical use, and does not generate drug resistance.

Methods and compositions for detecting and diagnosing sexually transmitted infections using a string of epitopes (SOE) specific for detection of causative microorganisms are provided. The antigenic epitopes may be single epitope sequences, a plurality of epitope sequences joined by amino acid linkers to form a series of epitopes (SOE), or nucleotide sequences encoding one or more SOEs and host cells harboring said SOE nucleotide sequences. SOEs specific for highly immunogenic regions of proteins from Trichomonas, Treponema and Neisseria species are provided. SOEs to detect the presence of Trichomonas species comprise regions from Trichomonas aldolase, GAPDH, α-enolase and α-actinin proteins. Pharmaceutical compositions comprising SOEs can also be used as vaccines or to elicit an immune response to specific microorganisms.

The Chinese medicine solution preparation for treating damp-heat syndrome is prepared with phellodendron bark, climbing groundsel, flavescent sophora root, sessile stemona root, clove and basil oil, mint oil and borneol in certain proportion as well as pharmaceutically acceptable carrier. The Chinese medicine solution preparation has the functions of clearing away heat and toxic material, drying to stop leukorrhagia, killing parasite to relieve itching, resisting inflammation and eliminating swelling, and thus has obvious effect on trichomonal vaginitis, candidal vaginitis, colpitis mycotica, cervicitis, etc. and no irritation and toxic side effect. The present invention also discloses the preparation process of the Chinese medicine solution preparation.

The present invention provides quantitative polymerase chain reaction (PCR) methods and kits for diagnosing trichomoniasis.

The release by trichomonads of a hydrolase that hydrolyzes a narrowly defined class of substrates at a low pH without interference from hydrolases that are unrelated to trichomoniasis is the basis for a selective diagnostic assay for trichomoniasis that measures hydrolysis of any of these substrates by vaginal fluid at a low pH. Selective assays for trichomoniasis are also obtained by removing particulate matter from a sample of vaginal fluid to extract a fraction devoid of particles greater than a selected size, and where desired, combining the extracted fraction with any of certain specified hydrolase inhibitors, then testing the fraction for enzymatic hydrolase activity. These qualities of trichomoniasis are the basis for a series of diagnostic tests and test devices that produce results that are detectable by visual and other means with a high degree of accuracy.

The invention discloses a formation mode of a chemical gradient sedimentation nano-silver coating on a stem cell culture interlayer support, which comprises the following steps: a, disinfecting the stem cell culture interlayer scaffold, and cleaning by high-frequency oscillation; b, putting the centrifugal container into a stirrer, starting the stirrer, at the speed of 20 r / min, slowly introducing the polar inert dispersion liquid , and carrying out chemical stabilization and dissolution to form a first solution; c, introducing nano-silver adhesive cement into the first solution in the step b, and uniformly stirring to form a second solution for later use; d, putting the stem cell culture interlayer bracket in the step a into a centrifugal container, and turning on a centrifugal machine for spin coating; and e, taking out the stem cell culture interlayer scaffold, vacuumizing and drying for 1-4 hours, and storing at 0-5 DEG C to prepare cells for later use. The stem cell culture interlayer support formed by the invention has the effect of killing bacteria, fungi, mycoplasma, chlamydia, spirochete and even trichomonad in clinical use, and does not generate drug resistance.

The invention discloses a Chinese herbal preparation for treatment of parasitic diseases in free range chickens. The Chinese herbal preparation is prepared from the following substances: radix aconitilateralis preparata, dried tangerine peel, resina ferulae, fructus chebulae, flos magnoliae, fingered citron, common club moss herb, folium viticis negundo and cortex moutan. The Chinese herbal preparation provided by the invention is prepared by reasonable combination of various Chinese herbal ingredients, has targeted control effect on parasitic diseases in free range chickens, can reduce the incidence of coccidium, trichomonas and leucocytozoon caulleryi to 1% or below, effectively guarantees the growth quality of free range chickens, and has substantial production and use value.

The invention relates to potent gynaecologic detoxifying capsules, which comprise the following main components by weight: 2 grams of borneol, 20 grams of artificial bezoar, 5 grams of anhydrous sodium sulphate, 10 grams of furazolidone tablets, 2 grams of camphor powder and 50 grams of lightyellow sophora root. The potent gynaecologic detoxifying capsules are suitable for various gynaecologic diseases, gonorrhoea, trichomonas, staphylococcus aureus, candida, herpes, non-gonococcus, mixed bacteria, vaginitis, cervicitis, endometritis, leucorrhea increased, yellow green leucorrhea and leucorrhea stench, and have the characteristics of low production cost and obvious action effect.