Formation mode of chemical gradient sedimentation nano-silver coating on stem cell culture interlayer support

A technology of stem cell culture and chemical gradient, which is applied in the field of chemical gradient sedimentation nano-silver coating in the formation of stem cell culture compartment scaffold, which can solve the problem of ineffective protection from being corroded by external microorganisms and failure to eliminate inflammatory reactions , accelerate wound healing, etc.

Inactive Publication Date: 2021-06-04
西安美丽起点生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The stem cell culture compartment scaffold in the prior art is an ordinary polymer material, which cannot effectively protect the stem cells from being eroded by external microorganisms, especially the erosive growth of bacteria during the process of culturing stem cells.
[0004] Moreover, the ordinary polymer culture spacer scaffold cannot imp

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047]Polyethylene oxide PEO material culture compartment support, high-frequency oscillation cleaning. Take the centrifuge container and put it in the mixer, turn on the mixer, 20 rpm, slowly introduce the DMF dispersion, (the DMF dispersion uses N,N-Dimethylformamide (N,N-Dimethylformamide), the DMF dispersion is used as a polar inert Solvent, colorless and transparent liquid, boiling point 153°C, relative density (water=1) 0.95, relative vapor density (air=1) 2.51, critical temperature 374°C, refractive index (25°C): 1.42817, viscosity (,25°C )0.802mPa·s, specific rotation 0.94°, electrical conductivity 6×10-8S / m, thermal conductivity C0.1657W / (m·K), 20°, topological molecular polar surface area (TPSA): 20.3. The process avoids the ultraviolet environment, introduces nano-silver glue, accounts for 0.18% of the total mass ratio of nano-silver, and stirs it evenly for later use. The culture compartment bracket is placed in the centrifuge container, and the centrifugation is t...

Embodiment 2

[0049] Polyethylene oxide PEO culture compartment support, high-frequency oscillation cleaning. Take the centrifuge container and place it in the mixer, turn on the mixer, 20 rpm, slowly introduce the DMF dispersion, (the DMF dispersion uses N-N dimethylacetamide and N-N dimethylformamide to mix according to 0.5:2), DMF The dispersion liquid is used as a polar inert solvent, and the topological molecular polar surface area (TPSA): 20. The whole process avoids the ultraviolet environment and introduces nano-silver glue. The nano-silver accounting for 0.19% of the total mass ratio is stirred evenly for later use. Place the culture interlayer support in a centrifuge container, turn on the centrifuge, and spin-coat at a rotation speed of 6500 rpm for 1 min to realize the loading of nano-silver. The above process was repeated twice to prepare a nano-film containing a nano-silver matrix accounting for 0.18% of the total mass, and the DMF dispersion was extracted. Take out the bas...

Embodiment 3

[0051] PEO-like polymer culture compartment support, high-frequency vibration cleaning. Take the centrifuge container and put it in the mixer, turn on the mixer, 20 rpm, slowly introduce the DMF dispersion, (the DMF dispersion uses N,N-Dimethylformamide (N,N-Dimethylformamide), the DMF dispersion is used as a polar inert Solvent, colorless and transparent liquid, boiling point 153°C, relative density (water=1) 0.95, relative vapor density (air=1) 2.51, critical temperature 374°C, refractive index (25°C): 1.42817, viscosity (,25°C )0.802mPa·s, specific rotation 0.94°, electrical conductivity 6×10-8S / m, thermal conductivity C0.1657W / (m·K), 20°, topological molecular polar surface area (TPSA): 20.3. The process avoids ultraviolet environment. Import nano-silver mucilage, account for the nano-silver of 0.18% of the total mass ratio, stir evenly for later use. Place the culture compartment support in the centrifuge container, open the centrifugation, the rotation speed is 6000rpm, ...

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Abstract

The invention discloses a formation mode of a chemical gradient sedimentation nano-silver coating on a stem cell culture interlayer support, which comprises the following steps: a, disinfecting the stem cell culture interlayer scaffold, and cleaning by high-frequency oscillation; b, putting the centrifugal container into a stirrer, starting the stirrer, at the speed of 20 r/min, slowly introducing the polar inert dispersion liquid , and carrying out chemical stabilization and dissolution to form a first solution; c, introducing nano-silver adhesive cement into the first solution in the step b, and uniformly stirring to form a second solution for later use; d, putting the stem cell culture interlayer bracket in the step a into a centrifugal container, and turning on a centrifugal machine for spin coating; and e, taking out the stem cell culture interlayer scaffold, vacuumizing and drying for 1-4 hours, and storing at 0-5 DEG C to prepare cells for later use. The stem cell culture interlayer support formed by the invention has the effect of killing bacteria, fungi, mycoplasma, chlamydia, spirochete and even trichomonad in clinical use, and does not generate drug resistance.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, biomedicine, nanotechnology, and chemical gradient sedimentation, and in particular to a method for forming a chemical gradient sedimentation nano-silver coating on a scaffold of a stem cell culture compartment. Background technique [0002] Nanometer (nm) is the smallest unit of measurement after the micron in the physics world, with the development of nanotechnology. A large number of nanomaterials have been found to have antiphysical phenomena. As well as the quality and effect of special functions, nano-silver is also after the extensive development of metal nano-materials. an emerging field of research. Silver in the nanometer state has produced a qualitative leap in the bactericidal ability used in biomedicine. A very small amount of nano-silver can produce a powerful bactericidal effect that was unimaginable in the past. Nano-silver can kill more than 650 kinds of bacteria in a few...

Claims

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Application Information

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IPC IPC(8): A61L27/30A61L27/54B05D1/00
CPCA61L27/306A61L27/54A61L2300/104A61L2300/404A61L2400/12A61L2420/02B05D1/005
Inventor 刘哲
Owner 西安美丽起点生物科技有限公司
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