80 results about "Spirochaete" patented technology

The invention discloses a breeding method of Spirulina alga species, which comprises the following steps: 1. selecting 5L of alga culture solution, filtering with a silk fabric to remove smaller algae; 2. carrying out mutagenesis with a phosphate buffer solution; 3. selecting single Spirulina with larger body and more spiral rings; culturing in a test tube, and calculating the growth rate; 4. culturing the Spirulina elements subjected to amplification culture in a 40001*12 DEG C lighting incubator; and 5. selecting the Spirulina element with the highest growth rate to obtain the mutant line subjected to mutagenesis screening, purification and cloning. The method changes the coarse breeding method of screening the alga species by the filter screen into capillary selected breeding; and the unicell screening is utilized to remove bad Spirulina individuals to obtain high purification, and the mutagenesis passivation is performed to obtain the high-quality high-growth-rate Spirulina alga species. The alga species purification and culture conditions are continuously optimized, so that the Spirulina yield is enhanced to 30-50%, and the product quality is further improved.

A method for raising clam worm includes such steps as choosing health parents, artificial fertilization, putting the fertilized ova in the seawater contained in drum, supplying seaweed (such as spirulina) as feed to culture larvae until the larva has 5-6 pairs of legs, culturing in spool in water flowing mode for 10 days while supplying seaweed and fish powder as feed, culturing in water exchanging mode while supplying fish powder as feed, and culturing in water bath while supplying seaweed and fish powder as feed.

The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic Borrelia, the use of the DNA sequences in recombinant vectors to express polypeptides, the encoded amino acid sequences, application of the DNA and amino acid sequences to the production of polypeptides as antigens for immunoprophylaxis, immunotherapy, and immunodiagnosis. Also disclosed are the use of the nucleic acid sequences as probes or primers for the detection of organisms causing Lyme disease, relapsing fever, or related disorders, and kits designed to facilitate methods of using the described polypeptides, DNA segments and antibodies.

The treatment of spirulina by a process which includes placing spirulina that has not been previously heat-sterilized, lactic acid bacteria and sugar in water, then culturing the lactic acid bacteria provides treated spirulina in which the distinctive taste and odor of spirulina are minimized, in which active ingredients such as phycocyanin remain intact, and which contains a reduced level of bacteria other than lactic acid bacteria.

The present invention discloses a method to identify and quantify environmental microorganisms useful in biomining processes. These microorganisms are basically 10, belonging to Bacteria: Acidiphilium sp., Leptospirillum sp., Sulfobacillus sp., Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans; and Archaea: Acidianus sp., Ferroplasma sp., Metallosphaera sp., Sulfolobus sp. and Thermoplasma sp.The method comprises performing a PCR with specific primers designed in our laboratories for different taxons SEQ ID No. 4 to SEQ ID No.: 407. With qPCR results and other data obtained from the analyzed sample, the microorganism concentration of each analyzed taxon present in the sample is calculated using a mathematical formula.

The invention provides a purification and breeding method of a spirulina species. The method comprises the following steps: (1) preparing 3L of a spirulina species culture solution; (2) filtering the culture solution; (3) transferring a spirulina gel, which is obtained from filtration, into a triangular flask, adding 200-300ml of medium L6, conducting adaptive restoration culture, and controlling environments as follows: illumination is 4000lx and temperature is 35 DEG C; (4) conducting alkaline cleaning on the spirulina gel; (5) cleaning the spirulina gel; (6) reserving supernatant spirulina of liquid, adding 50-100ml of medium L6, and uniformly mixing the materials; (7) diluting an obtained mixture; (8) transferring 1ml of the liquid to a sterilized 1-5ml centrifuge tube, and conducting adaptive restoration culture on the centrifuge tube in a water bath of 2000lx and 30 DEG C for 12h; and (9) selecting large individual spirulina having a relatively high amount of spirals by virtue of a single-filament separator, and conducting enlarge cultivation on the large individual spirulina having a relatively high amount of spirals so as to achieve the large-scale production of the spirulina species. With the application of the method, by effectively conducting multi-stage enlarge cultivation on single-filament algae, pure species free from miscellaneous bacteria or miscellaneous algae can be obtained.

The invention provides a novel spirulina polypeptide P1 with bacteriostatic activity. An amino acid sequence of the spirulina polypeptide P1 is shown as Seq ID No.1. The spirulina polypeptide P1 with bacteriostatic activity is extracted from spirulina for the first time. Experiments demonstrate that the spirulina polypeptide P1 can effectively suppress gram-positive bacteria and gram-negative bacteria, has no toxic or side effect, can be used as a broad-spectrum antibacterial agent or a bacteria preservative, and has wide application prospects.

The invention discloses a poly-spirulina peptide biostimulant, and belongs to the field of an agricultural fertilizer synergist. Domesticated and separated fermentation strains are used for fermentingspirulina so as to prepare the poly-spirulina peptide biostimulant; the fermentation strains are any one kind of bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, paenibacillus mucilaginosus and bacillus lateralis. The poly-spirulina peptide biostimulant is mainly prepared through biological fermentation; no chemicals are added; safety and environment protection are realized;the fertilizer utilization rate and the soil enzymatic activity can be improved; medium trace elements in the soil can be supplemented; the crop rooting and nutrition absorption can be promoted; thesoil environment is improved; the harmful bacteria of soil are inhibited.

Antigenic polypeptides comprising linear immunodominant epitopes of Borrelia outer surface protein A (OspA) or Borrelia outer surface protein C (OspC) are useful as vaccines against Lyme disease, and as diagnostics for detecting Borrelia infections. The OspA and OspC antigenic polypeptides typically comprise a plurality of peptides representing epitope containing regions from multiple distinct phyletic groups. The antigenic polypeptides may also include epitopes from both Borrelia OspA and Borrelia OspC.

A reagent for microscopy-based detection of pathogens, especially spirochetes, from body fluids characterized by that containing the following ingredients: tetracain (125-200 mg / l), mannit (1500-2000 mg / l), EGTA (etilene bis[oxyetiline-nitrilo]-tetraacetate), 0.76-0.114 mg / l magnesium-chloride (preferably in amount of 0.10 mg / l), caffeine-sodium-benzoate (2000-4000 mg / l), glucose (1800-2200 mg / l), glycerol (75-105 mg / l), optimally tri-sodium-citrate (preferable in an amount of 10000 mg / l), Hoechst 33342 dye (1.11 mg / l), if required 20 to 40 ml of distilled water and RPMI 1640 culture media to make 100 ml. Also, a method for detecting pathogens.

The invention relates to a kit for detecting Lyme bacteria in tick acarina and a detection method thereof. The kit for detecting the Lyme bacteria in the tick acarina at least comprises: Biodyne C membrane covalently bonded with universal probes of Lyme bacteria spirochetes and probes for specific oligonucleotide, B. burgdorferi sensu stricto, B. garinii, B. afzelii and B. valaisiana and a pair of primers which are designed on conservative regions at two ends of a gene sequence of spirochete 5S-23S rRNA and have 225 bp fragment that can be amplified, one primer of which is marked with biotin.

The invention discloses an LAMP primer combination for detecting three ophthalmic infection spirochetes and application. The primer combination is composed of eighteen single-stranded DNA molecules shown from the sequence 1 to the sequence 18. The invention further provides application of the primer combination. The invention furthermore provides a method of using the primer combination for identifying whether a spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira, a method for identifying whether the spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira and a method for identifying whether a sample to be detected is infected by treponema pallidum and / or borrelia burgdorferi and / or leptospira. Treponema pallidum, borrelia burgdorferi and leptospira can be fast and accurately detected by using the method.

A bacterin including effective immunizing amounts of two non-crossprotective isolates of inactivated Borrelia burgdorferi, an adjuvant in an amount effective to enhance the immunogenicity of the inactivated Borrelia burgdorferi isolates and a suitable carrier is provided herein. The bacterin may also contain a third non-crossprotective isolate. A bacterin including effective immunizing amounts of an antigenic subunit derived from a first Borrelia burgdorferi isolate and a second, non-crossprotective Borrelia burgdorferi isolate, an adjuvant in an amount effective to enhance the immunogenicity of the antigenic subunits and a suitable carrier is also provided. The bacterin may also contain an effective immunizing amount of an antigenic subunit of a third Borrelia burgdorferi. Further provided is a bacterin which includes effective immunizing amounts of two noncrossprotective isolates of inactivated Borrelia burgdorferi and one or more antigenic subunits from the non-crossprotective isolates, an adjuvant in an amount effective to enhance the immunogenicity of the inactivated Borrelia burgdorferi and antigenic subunits and a suitable carrier.

A novel isolated Borrielia burgdorferi sensu lato surface antigen is characterized by a relative molecular mass of 39.5 kDa. This antigen is expressed in vitro by spirochetes of a B. burgdorferi sensu lato strain. This antigen induces antibodies which kill spirochetes of a B. burgdorferi sensu lato strain by ADCK in vitro. Novel Borrelia cassette string protein or fragments thereof are also useful, as is the P39.5 protein in diagnosing Lyme disease and in compositions for treatment or prophylaxis thereof.

A degerming dustproof a multifunctional electronic mask is characterized in that an outer layer body 1, an inner layer body 2, an isolation layer 3, an air hole 4, an air hole 5, an air hole 6, a negative electrode 7, an adsorption body 8, a positive electrode 9, an adsorption body 10, a supporting body 11, ear bands 12, a power supply 13 and a zipper 14 are connected into a whole to form the degerming and dustproof multifunctional electronic mask. The degerming dustproof multifunctional electronic mask which is wide in degerming range and various in dustproof variety. Degerming comprises non-cell microorganisms (various viruses); prokaryotic cell type microorganisms (various bacteria, mycoplasma, chlamydia, rickettsia, spirochete and actinomycetes); eukaryotic cell type microorganisms (fungi) and various bacterial spores. Dust prevention includes a variety of oily, non-oily contaminants in air from particles greater than 5 microns to particles less than 1 micron. The degerming dustproof multifunctional electronic mask is suitable for medical treatment and public health, production labor, household protection, fitness and physical exercise. The degerming dustproof multifunctional electronic mask is wide in manufacturing material and low in production cost, the mask body is made of elastic non-breathable ultrathin insulating material fibers, fabrics and films, the electrodes aremade of metal sheets, metal coating films or conductive carbon fibers, and the adsorption body is made of paper fibers (used in dry seasons in winter) or chemical fibers (used in rainy seasons in summer). The power supply is a general mobile phone lithium battery and a high-voltage electron generator.

The present invention relates to a composition comprising Brachyspira hyodysenteriae bacteria, particularly in the field of immunization against swine dysentery. The composition of the invention may comprise bacteria from at least two genetically diverse strains of B. hyodysenteriae. The composition of the invention may also comprise bacteria of a strain deposited at the Collection Nationale de Cultures de Microorganismes (CNCM), Institut Pasteur, on Mar. 14, 2013, with registration number CNCM I-4720. The invention relates also to the composition of the invention for use as a vaccine, preferably a universal vaccine against swine dysentery caused by B. hyodysenteriae.

Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria.

The present invention discloses the application of L-arginine, L-ornithine and L-citrulline as spirulina princeps growth regulator, and aims at promoting the growth of spirulina princeps for cultivating spirulina princeps, L-arginine, L-ornithine or L-citrulline is added into culture liquid to speed the growth of spirulina princeps, raise the yield of spirulina princeps. Test shows that adding L-arginine, L-ornithine or L-citrulline in 0.2-5 g / L into the culture liquid can raise the yield of spirulina princeps by 8-52 % and speed the growth of spirulina princeps by 10-20 %.

The invention provides a cultivation method of spirulina platensis in a saline stress environment, and belongs to the field of biotechnology, which comprises the following steps: in the saline stressenvironment, performing salt-tolerant acclimation cultivation on the of spirulina platensis; placing the acclimated and cultivated spirulina in an environment with an elevated oxygen partial pressurefor cell differentiation cultivation; and performing further extended cultivation, and collecting the obtained salt-tolerant and oxygen-tolerant spirulina species. In the above saline stress environment, the initial salt concentration is 0.1-1%, the final concentration is 3.5-5%. The cultivation method provided in the invention may perform stimulant cultivation at an early stage of cells, which improves the algae superoxide dismutase activity, the peroxidase activity and the catalase activity, increases the algal filament thread pitch and the length in order to improve the biomass density andthe filtering and harvesting efficiency, reduces the malondialdehyde production, and promotes increase of algal phycocyanin and polysaccharide contents, facilitating industrial and oriented acquisition of nutrients including phycocyanins and polysaccharides.

The invention belongs to the technical field of biocatalysis, and particularly relates to construction and application of genetically engineered bacteria for one-step conversion from cheap substrate phytosterol (PS) as a raw material to 5alpha-androstanedione (5alpha-AD). The 5alpha-reductase gene from treponema pallidum is heterologously expressed into mycobacteria mainly producing androstenedione (AD), and one-step biotransformation from phytosterol to 5alpha-AD is realized, so that green and efficient production of 5 alpha-AD is realized.

Spirulina princeps contained L-arginine, L-ornithine,and L-citrullin is cultured. General spirulina has no L-arginine, L-ornithine and little L-citrullin contained so as not to meet the needs of human health. This invention emphasizes that domesticate spirulina strain, culturing it in a small pond, then cultivating them in a middle pond then a big pond, adding the 0.05 - 6g L-arginine, L-ornithine, L-citrullin into per liter culturing liquid. Through the absorbing and transforming and biological metabolism by spirulina, the additives exist in the cells of spirulian and becomes parts of the composition.The L-arginine, L-ornithine, L-citrulli contents reached 4-15%, and could meet the needs of human health, also their existing states are easy malabsorbing by human.

The invention provides a nested fluorescent quantitative PCR (polymerase chain reaction) detection method for Lyme disease spirochetes. According to the invention, rrf-rrl is taken as a target gene, nested PCR primers and probes for detecting lyme disease spirochetes are designed, the nested PCR primers comprise a first round of PCR primers and a second round of PCR primers, and the probes are introduced in the second round of PCR reaction so as to improve the detection sensitivity. The nested fluorescent quantitative PCR detection method for Lyme disease spirochetes provided by the invention has better specificity and high sensitivity, and the lower detection limit reaches 0.01 pg / [mu]L. According to the method disclosed by the invention, a positive sample can be subjected to single-point typing, so that the effective discrimination of four types of Borrelia burgdorferi, namely, Borrelia burgdorferi sensu stricto (B.b.s.s), Borrelia garinii (B.g), Borrelia afzelii (B.a), and Borrelia valaisiana (B.v) can be realized.

Presented herein are compositions and methods for the long-term in vitro culturing of Treponema species such as T. pallidum. Culture media and systems for Treponema culture are also provided.