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Poly-spirulina peptide biostimulant

A technology of biostimulants and spirulina peptides, applied in the directions of microorganisms, microorganism-based methods, and biological organic part treatment, etc., can solve the problems of low absorption and utilization rate of crops, affecting the nutrient content of fertilizers, and rapid nutrient loss, etc. Improve fertilizer utilization and soil enzyme activity, promote phosphorus absorption, and improve the effect of soil environment

Inactive Publication Date: 2018-05-22
迪斯科科技集团(宜昌)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, my country's fertilizer application has the characteristics of large amount of fertilization, excessive nutrient loss, low crop absorption and utilization rate, and problems such as environmental pollution and resource waste caused by excessive fertilization.
At the same time, the existing fertilizer synergists are produced using inorganic raw materials or artificially synthesized polymer materials, which have problems such as high cost, poor effect, toxicity, and affecting the nutrient content of fertilizers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A polyspirulina peptide biostimulant is prepared by fermenting spirulina with domesticated and isolated Bacillus subtilis.

[0048] The water content of spirulina is 9.6%, and the mass percentage of each component is 17% of carbohydrates, 72% of protein, 1.2% of chlorophyll, 5.0% of amino acids and lysine, and 10% of total minerals. %.

[0049] The method for domestication and separation of fermentation strains is as follows:

[0050] A. Soak the unbroken dry spirulina in water for 20h and pull out and add sodium chloride and sucrose. The mass ratio of the added sodium chloride and sucrose to the unbroken dry spirulina is 1:2:18. Cook at 0.1MPa for 40min to obtain the primary culture medium;

[0051] B. Inoculate the fermentation strains on the primary selection medium, and after cultivating at a constant temperature at 36°C for 24 hours, select colonies that are brown, have wire drawing phenomenon, and have relatively obvious transparent circles and inoculate them in...

Embodiment 2

[0059] A polyspirulina peptide biostimulant is prepared by fermenting spirulina with domesticated and isolated bacillus licheniformis.

[0060] The water content of spirulina is 9.8%, and the mass percentage of each component is 16% of carbohydrates, 73% of protein, 1.3% of chlorophyll, 4.8% of amino acids and lysine, and 11% of total minerals. %.

[0061] The method for domestication and separation of fermentation strains is as follows:

[0062] A. Soak the unbroken dry spirulina in water for h and then pull it out and add sodium chloride and sucrose. The mass ratio of the added sodium chloride and sucrose to the unbroken dry spirulina is 1:3:22. Under 0.3MPa, cook for 55min to obtain primary culture medium;

[0063] B. Inoculate the fermentation strains on the primary selection medium, and after cultivating at a constant temperature at 38°C for 24 hours, select colonies that are brown, have wire drawing phenomenon, and have a relatively obvious transparent circle and inocu...

Embodiment 3

[0071] A polyspirulina peptide biostimulant is prepared by fermenting spirulina with domesticated and isolated bacillus amyloliquefaciens.

[0072] The water content of spirulina is 9.7%, and the mass percentage of each component is 18% of carbohydrates, 72% of protein, 1.4% of chlorophyll, 4.9% of amino acids and lysine, and 9% of total minerals. %.

[0073] The method for domestication and separation of fermentation strains is as follows:

[0074] A. After soaking the dry spirulina with unbroken walls in water for 24h, pull out and add sodium chloride and sucrose, the mass ratio of the added sodium chloride and sucrose to the dry spirulina with unbroken walls is 1:2:20. Cook at 0.2MPa for 50min to obtain the primary culture medium;

[0075] B. Inoculate the fermentation strains on the primary selection medium, and after cultivating at a constant temperature at 37°C for 24 hours, select colonies that are brown, have wire drawing phenomenon, and have relatively obvious trans...

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Abstract

The invention discloses a poly-spirulina peptide biostimulant, and belongs to the field of an agricultural fertilizer synergist. Domesticated and separated fermentation strains are used for fermentingspirulina so as to prepare the poly-spirulina peptide biostimulant; the fermentation strains are any one kind of bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, paenibacillus mucilaginosus and bacillus lateralis. The poly-spirulina peptide biostimulant is mainly prepared through biological fermentation; no chemicals are added; safety and environment protection are realized;the fertilizer utilization rate and the soil enzymatic activity can be improved; medium trace elements in the soil can be supplemented; the crop rooting and nutrition absorption can be promoted; thesoil environment is improved; the harmful bacteria of soil are inhibited.

Description

technical field [0001] The invention relates to a biostimulant, in particular to a biostimulant containing polyspirulina peptide, and belongs to the field of agricultural fertilizer synergists. Background technique [0002] my country is a big country in the production and use of chemical fertilizers. According to the data of the National Bureau of Statistics, in 2013, the production of chemical fertilizers was 70.37 million tons, and the amount of agricultural chemical fertilizers used was 59.12 million tons. The current situation of my country's cultivated land is that the basic soil fertility is low, and the application of chemical fertilizers contributes more to the increase in grain production, accounting for more than 40%. Therefore, "weight loss and efficiency increase" and "high yield and high efficiency" are the way to sustainable development. Only by continuously improving the quality of cultivated land , Gradually increase the utilization rate of fertilizers, and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05F17/00C12N1/20C12N1/36C12R1/07C12R1/10C12R1/125C12R1/01C05G3/80
CPCC05C11/00C05F11/00C05F17/00C12N1/20C12N1/36C05G3/80C05F11/02C05F11/08Y02W30/40
Inventor 李明舵陈家辉张想雷会霄
Owner 迪斯科科技集团(宜昌)有限公司
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