59 results about "Paenibacillus mucilaginosus" patented technology

The present invention belongs to microbial polysaccharide fermentation, and particularly relates to a method for producing a microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus, wherein Paenibacillus mucilaginosus is inoculated in a culture medium containing a carbon source and a nitrogen source to carry out fermentation, and bacteria heat stimulation, addition of a surfactant during a fermentation process, and other manners are performed to optimize the production process. With the present invention, the problem of low yield of polysaccharides in the fermentation broth in the prior art is solved, and advantages of high polysaccharide yield in the fermentation broth, good bacterial growth, short fermentation period and the like are provided.

The invention relates to the technical field of microbial agents, and in particular to a microbial preparation as well as a preparation method and application of the microbial preparation. The microbial preparation is prepared by adopting and fermenting paenibacillus mucilaginosus, and a fermented culture solution contains shrimp and crab shells, collagen, sodium chloride, amino acid, carbohydrate and compound fertilizer, so that the microbial preparation has the function of inhibiting harmful germs; according to the microbial preparation disclosed by the embodiment of the invention, spray application and root irrigation can be carried out on rose and lycium chinense which suffer from a root rot disease and banana which suffers from a panama disease, and a result shows that the microbial preparation provided by the invention has a good prevention and treatment function on the root rot disease of the rose and the lycium chinense and the prevention effect can be up to 85.3 percent and 69.0 percent; the microbial preparation has a good prevention and treatment function on a blight disease of the banana, and the prevention effect can be up to 85.0 percent.

The invention discloses a Paenibacillus mucilaginosus high producing strain KN-18. The Paenibacillus mucilaginosus high producing strain KN-18 is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO:M2014321. The strain has extremely strong silicon releasing and spore producing ability, and the silicon releasing ability of a strain broth can reach 26.8[mu]g/ml, and is substantially better than the silicon releasing ability of an original strain and other Paenibacillus mucilaginosus. The invention also discloses a culturing method of the Paenibacillus mucilaginosus high producing strain. The spore number of the broth obtained through the method can reach 2 billions/ml, and is substantially better than that of other culturing methods. The Paenibacillus mucilaginosus can be used in rice as a biological fertilizer to increase the stem height of the rice by above 5%, increase the length of spike of rice by above 4%, increase the number of filled grains by above 7% and the yield per 666.667m<2> by above 9%; and the Paenibacillus mucilaginosus can be used in muskmelon to make the muskmelon mature 2d earlier, increase the melon number per plant by 0.1, increase the weight per melon by above 5% and the yield per 666.667m<2> by above 9%.

The invention relates to the field of microbial agents, and particularly discloses a saline alkali soil remediation microbial agent and a preparation method thereof.The saline alkali soil remediationmicrobial agent is prepared from, by weight, 25-30 parts of microbial agent carriers, 10-15 parts of composite microbial agents and 25-30 parts of biochemical organic acid, the complex microbial inoculant comprises Bacillus amyloliquefaciens, Paenibacillus mucilaginosus, Bacillus megaterium and Trichoderma harzianum, wherein the Bacillus amyloliquefaciens, the Paenibacillus mucilaginosus, the Bacillus megaterium and the Trichoderma harzianum are mixed according to a mass ratio of (1-2): (1-2): (1-2): 5. The microbial inoculum carrier is attapulgite, and the biochemical organic acid comprises biochemical fulvic acid and brown humic acid which are mixed in equal mass. The components of the microbial agent are optimized, the defects of a single-strain microbial agent are overcome, meanwhile,attapulgite is adopted as a matrix, organic acid is used as an auxiliary improvement material in an auxiliary mode, the saline-alkali soil remediation rate is improved, the improvement service life ofthe saline-alkali soil remediation agent is prolonged, and finally the saline-alkali soil is effectively improved.

The invention belongs to the technical field of tea leaf planting, and in particular relates to a special soil improver for tea leaf planting and a preparation method thereof. By combining porous structures of diatomite, cotton stalk charcoal and rice husk charcoal and adsorbing beneficial bacteria paenibacillus mucilaginosus, streptomyces griseus and photosynthetic bacteria, the prepared specialsoil improver for tea leaf planting can improve the problems of soil acidification and hardening, so that the granular structure of soil is good and loose, and the bio-availability of heavy metals inthe soil is reduced. The improver promotes development and robust growth of crop root systems, enhances the cold resistance, drought resistance, disease resistance and stress resistance of the crops,and improves the output of the tea leaves and the quality of the tea leaves.

The invention provides a biological fertilizer and a preparation method thereof. The preparation method comprises the steps of: mixing peanut shells, soybean meal and edible fungus residues, conducting steam explosion and solid-liquid separation, and collecting a solid material A and a liquid material A respectively; adding oyster shell powder, livestock and poultry manure, a vegetable leaf and fruit mixture and earthworms into the solid material A, performing composting treatment, and screening out the earthworms to obtain a solid material B; adding potassium fulvate and molasses liquid into the solid material B, inoculating trichoderma and paecilomyces lilacinus, and performing solid fermentation to obtain a solid material C; taking the screened earthworms, crushing, filtering, and collecting filtrate; mixing the liquid material A, the filtrate, brown sugar, a fish protein liquid fertilizer and a multi-element chelating microelement fertilizer, inoculating paenibacillus polymyxa, paenibacillus mucilaginosus and lactic acid bacteria, and performing liquid fermentation to obtain a liquid material B; and mixing the solid material C and the liquid material B, granulating, drying and dewatering. When the biological fertilizer is used for producing kiwi fruits, the fruit quality is good.

The invention relates to the technical field of soil amelioration, and in particular relates to a Chinese herbal medicine microbial soil conditioner. The soil conditioner comprises the following raw materials, in parts by weight: 80-100 parts of turfy soil, 20-25 parts of sodium alginate, 15-25 parts of coconut shells, 26-35 parts of mulberry leave extraction waste, 20-30 parts of herba houttuyniae, 15-25 parts of loofah sponge, 25-40 parts of shell powder, and 10-20 parts of a microbial composite agent, wherein the microbial composite agent is composed of halophilic bacteria and bacilli, andthe bacilli include paenibacillus mucilaginosus, bacillus licheniformis, paenibacillus polymyxa, and bacillus subtilis in a mass ratio of 1.1:1.5:1.0:1.2. According to the soil conditioner provided bythe invention, the Chinese herbal medicine herba houttuyniae, the mulberry leave extraction waste and the microbial composite agent are added, so that the problems that a current soil conditioner hasa single function and a non-ideal improvement effect are solved.

The invention belongs to preparation of biological polysaccharide, and particularly refers to a method for producing microbial flocculant by using Paenibacillus mucilaginosus. The method comprises the following steps of: A, performing bacterial domestication on the Paenibacillus mucilaginosus ACCC10013 by means of thermal shock; B, aerobically culturing the Paenibacillus mucilaginosus ACCC10013 domesticated in the step A in a sterile fermental cultivation medium containing carbon source, nitrogen source and growth factors, thus obtaining a fermentation broth containing microbial flocculant; C, separating the fermentation broth containing microbial flocculant, prepared in the step B, through a macroporous adsorption resin to produce the microbial flocculant. The method provided by the invention solves the problems, such as high cost and low purity of flocculant, of the existing technology, and has the advantages of low technological cost of preparation process, high yield, high product purity, high activity, and the like.

The invention discloses microbial powder for preventing and treating strawberry root rot and a preparation method thereof, and belongs to the field of agricultural microorganisms. The microbial bacterial powder for preventing and treating the strawberry root rot disease is prepared from the following components in parts by weight: 22 to 27 parts of bacillus subtilis bacterial powder, 23 to 28 parts of paenibacillus mucilaginosus bacterial powder, 21 to 26 parts of paecilomyces lilacinus bacterial powder and 22 to 28 parts of trichoderma harzianum bacterial powder. The bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCCNO.17701; the paenibacillus mucilaginosus is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.17702. The strawberry root rot prevention and treatment effect is good, and the yield and quality of strawberries can be remarkably improved.

The invention provides a composite microbial agent capable of improving soil fertility, and a preparation method and application thereof. The composite microbial agent comprises, by volume, 80 to 95%of composite bacterial liquid and 5 to 20% of biostimulant, wherein the composite bacterial liquid is composed of Bacillus megaterium fermentation broth and Paenibacillus mucilaginosus fermentation broth; each fermentation broth contains more than 5*10<8> CFU/mL of effective live bacteria; and the composite bacterial liquid contains 1*10<9> CFU/mL of effective live bacteria altogether. The composite microbial agent provided by the invention plays an important role in improving soil fertility, alleviating soil salinization, reducing resource wastage and increasing crop yield.

The invention discloses a high-potassium-solubilizing paenibacillus mucilaginosus strain. The high-potassium-solubilizing paenibacillus mucilaginosus strain is paenibacillus mucilaginosus GT018-JZ18112 and is preserved in the China General Microbiological Culture Collection Center on May 7, 2019, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.17702. The high-potassium-solubilizing paenibacillus mucilaginosus strain has excellent potassium-dissolving capacity and can improve the soil fertility.

The invention relates to a microorganism for producing chitosanase, and a plant cultivation composition and cultivation method using the same, in particular to a lettuce cultivation method based on Paenibacillus mucilaginosus C23 (collected under KACC91963P) and Bacillus ehimensis (collected under KACC91964P); compared with traditional chitosan-decomposing microorganisms, the microorganism used in the method provided herein provides decomposition at higher speed; when a chitosan fermentation using the microorganism applies to lettuce cultivation, the microorganism promotes the growth and development of lettuce and adds more functional ingredients and functions (polyphenols, flavonoids and electron donating capacity).

The invention relates to the field of microbial fertilizers, in particular to a high-nitrogen compound microbial fertilizer as well as a preparation method and application of the high-nitrogen compound microbial fertilizer. Thehigh-nitrogen compound microbial fertilizer comprises amicrobial fermentation inoculant and potassium fertilizer. The microbial fermentation inoculant is prepared by the following steps: inoculating paenibacillus polymyxa, bacillus subtilis and paenibacillus mucilaginosus intoa microbial fermentation culture medium for fermentation culture, and obtaining the microbial fermentation inoculant after fermentation is finished, the microbial fermentation culture medium comprises the following substances in percentage by mass: 50-80g/L of urea; 210-300 g/L of soybean meal; 30-50 g/L of corn flour; 0.5-30 g/L of calcium chloride; 3-5 g/L of dipotassium phosphate; 0.1 to 10g/L of magnesium sulfate; 0.1 to 10 g/L of manganese sulfate; and water. According to the high-nitrogen compound microbial fertilizer disclosed by the invention, mixed fermentation of various microorganisms does not have an antagonism effect, the activity of a single strain can be obviously improved, the fermentation period is shortened to 2d, and meanwhile, the conversion rate of urea and soybean meal is increased.

The invention discloses a microbial saline-alkali soil conditioner as well as a preparation method and application thereof, and belongs to the technical field of soil improvement. The improver comprises a carrier and compound microorganisms, the carrier comprises the following components in percentage by mass: 10-15% of attapulgite powder, 8-12% of humic acid and the balance of organic fertilizer, and the compound microorganisms comprise bacillus subtilis, actinomycetes and paenibacillus mucilaginosus. Compared with the prior art, the conditioner has the advantages that the conditioner is applied to saline alkali soil to be improved, the application amount is 150 kg/mu, the conditioner is applied to a crop growth cycle, the soil pH can be effectively reduced by 0.1-0.5 unit, and the soil bulk density is reduced by 10%.

The invention discloses an acid soil conditioner and a preparation method thereof and solves the problem that the prior art is undesirable in soil pH improvement, little in soil organic matter contentincrease and incapable of improving soil heavy metal pollution. The acid soil conditioner comprises alakaline organic matter and microorganisms, the microorganisms are selected from one or multiple of actinomycetes, bacillus subtilis, bacillus laterosporus, paenibacillus mucilaginosus and bacillus amyloliquefaciens, organic matter content is greater than or equal to 45%, N, P and K total contentis greater than or equal to 5%, and active viable count is not less than 0.5 billion/g. The acid soil conditioner can effectively improve soil pH, can increase soil organic matter content, supplementorganic nitrogen, phosphorus and potassium and mineral elements, increase soil beneficial microorganism flora amount and dissolve, adsorb and sediment soil heavy metal pollutants.

The invention provides an oak plant root-promoting microbial microcapsule inoculant and a preparation method thereof. The active ingredients of the microbial microcapsule inoculant are rhizobium radiobacter, bacillus megaterium, bacillus marinus and paenibacillus mucilaginosus, and the microbial microcapsule inoculant is long in normal-temperature storage time and high in thallus survival rate andcan promote growth of roots and tips of oak plants; the microbial microcapsule inoculant is prepared by adopting a spray drying technology, and the production process is reasonable in design, so thatthe cleanliness and sterility of the inoculant are guaranteed.

The invention discloses a multiple species inoculant for enhancing sediment nutrient. The multiple species inoculant comprises azotobacter chroococcum, bacillus megaterium and paenibacillus mucilaginosus; a volume ratio of bacteria solution of the azotobacter chroococcum to bacteria solution of the bacillus megaterium to bacteria solution of the paenibacillus mucilaginosus is 3:1:1. According to the invention, a segmented aerated culture method is adopted to carry out culture expansion, and the temperature is also changed with time of bacteria solution culture. The multiple species inoculant disclosed by the invention has the beneficial effects that the bacterial proportion of the multiple species inoculant can effectively enhance the sediment nutrient of an organic fertilizer, and the organic fertilizer can be decomposed to a great degree so as to enable crops to adsorb the organic fertilizer; compared with a multiple species inoculant which does not adopt segmented aerated culture, the multiple species inoculant adopting segmented aerated culture is more beneficial to bacterium breeding, enables a living bacteria count to be improved, and is beneficial for forming bacillus stearothermophilus spores; according to the multiple species inoculant disclosed by the invention, in different periods, different temperatures are adopted to culture bacillus, which enables the living bacteria count to be improved and is beneficial for forming spores.

The invention relates to a microbial fertilizer capable of reducing the amount and increasing the efficiency of a chemical fertilizer as well as a preparation method and application of the microbial fertilizer. The microbial fertilizer capable of reducing the amount and increasing the efficiency of a chemical fertilizer comprises biochar, a compound fermentation inoculant, a compound functional inoculant and an organic material; the compound fermentation inoculant comprises bacillus subtilis powder, streptococcus thermophilus powder and kluyveromyces lactis powder; and the compound functional bacterial agent is prepared from paenibacillus mucilaginosus powder, rhodopseudomonas acidophila powder and bacillus methylotrophicus powder. The microbial fertilizer is stable in fertilizer efficiency, can reduce the application amount of a chemical fertilizer, can promote plant growth, reduce the prevalence rate and death rate of plants, improve the crop yield and increase economic income, and has important application value in vegetable planting.