Selenium-rich microbial agent and preparation method thereof

A technology of selenium-enriched microorganisms and compound microbial agents, which is applied in fertilizer mixtures, organic fertilizers, layered/coated fertilizers, etc., can solve problems such as poor absorption efficiency of inorganic selenium, improve initial reproductive performance, and improve binding effects , Improve the effect of the rhizosphere ecosystem

Pending Publication Date: 2022-03-11
JIANGSU ZHIFENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to improve the defect of poor plant absorption efficiency of inorganic selenium, the application provides a selenium-enriched microbial bacterial agent and its preparation method

Method used

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  • Selenium-rich microbial agent and preparation method thereof
  • Selenium-rich microbial agent and preparation method thereof
  • Selenium-rich microbial agent and preparation method thereof

Examples

Experimental program
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Effect test

preparation example 1

[0051] A MOF-based hydrogel matrix:

[0052] (1) Add 1.5kg of cellulose hydrogel and 50kg of 0.1mol / L sodium selenite solution to 100kg of cetyltrimethylammonium bromide, stir and mix and ultrasonically disperse at 200W to collect the dispersion , and then add 10 kg of ascorbic acid aqueous solution to the dispersion liquid, heat up to 55 ° C, add 3 kg of 0.1 mol / L sodium hydroxide solution, stir and mix, wash with deionized water until the washing liquid is neutral, and prepare a gel liquid ;

[0053] (2) Add 1kg of gel solution and 12kg of absolute ethanol to 20kg of N,N-dimethylformamide, stir and mix and ultrasonically disperse at 200W, collect the dispersion mixture and add 0.5kg of zinc nitrate and 0.2kg of Phthalic acid, after heating and reacting at 120°C, let it stand and cool to room temperature, wash it with deionized water, and dry it until the mass fraction of sodium selenite is 1%, then the MOF-based hydrogel matrix 1 can be prepared .

preparation example 2

[0055] A MOF-based hydrogel matrix:

[0056] (1) Add 1.5kg of cellulose hydrogel and 50kg of 0.1mol / L sodium selenite solution to 100kg of cetyltrimethylammonium bromide, stir and mix and ultrasonically disperse at 200W to collect the dispersion , and then add 10 kg of ascorbic acid aqueous solution to the dispersion liquid, heat up to 55 ° C, add 3 kg of 0.1 mol / L sodium hydroxide solution, stir and mix, wash with deionized water until the washing liquid is neutral, and prepare a gel liquid ;

[0057] (2) Add 1kg of gel solution and 12kg of absolute ethanol to 20kg of N,N-dimethylformamide, stir and mix and ultrasonically disperse at 200W, collect the dispersion mixture and add 0.5kg of zinc nitrate and 0.2kg of Phthalic acid, after heating and reacting at 120°C, let it stand and cool to room temperature, wash it with deionized water, and then dry it until the mass fraction of sodium selenite is 10%, then the MOF-based hydrogel matrix 1 can be prepared .

preparation example 3

[0059] A MOF-based hydrogel matrix:

[0060] (1) Add 1.5kg of cellulose hydrogel and 50kg of 0.1mol / L sodium selenite solution to 100kg of cetyltrimethylammonium bromide, stir and mix and ultrasonically disperse at 200W to collect the dispersion , and then add 10 kg of ascorbic acid aqueous solution to the dispersion liquid, heat up to 55 ° C, add 3 kg of 0.1 mol / L sodium hydroxide solution, stir and mix, wash with deionized water until the washing liquid is neutral, and prepare a gel liquid ;

[0061] (2) Add 1kg of gel solution and 12kg of absolute ethanol to 20kg of N,N-dimethylformamide, stir and mix and ultrasonically disperse at 200W, collect the dispersion mixture and add 0.5kg of zinc nitrate and 0.2kg of Phthalic acid, after heating and reacting at 120°C, let it stand and cool to room temperature, wash it with deionized water, and then dry it until the mass fraction of sodium selenite is 20%, then the MOF-based hydrogel matrix 1 can be prepared .

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Abstract

The invention relates to the field of microbial agent materials, and particularly discloses a selenium-rich microbial agent and a preparation method thereof. The selenium-enriched microbial agent comprises the following substances in parts by weight: 5-10 parts of a compound microbial agent; 3 to 6 parts of peptone; 2-3 parts of yeast powder; 6-10 parts of a sodium selenite solution; 150 to 200 parts of water; the compound microbial agent comprises at least two of a bacillus subtilis microbial agent, a bacillus amyloliquefaciens microbial agent and a paenibacillus mucilaginosus microbial agent. The preparation method comprises the following steps: S1, immobilizing the microbial inoculum; s2, selenium enrichment treatment; and S3, blending preparation. The combination mode of the selenium-rich microbial agent material is optimized, water is adopted for dispersion, the problem that the combination performance of a traditional microbial agent and an inorganic selenium material is poor is solved, inorganic selenium is effectively dispersed in water and is effectively combined with the microbial agent, and the uniform and stable performance of the selenium-rich microbial agent is improved.

Description

technical field [0001] The present application relates to the field of bacterial agent materials, in particular to a selenium-enriched microbial bacterial agent and a preparation method thereof. Background technique [0002] Selenium is one of the essential trace elements for the human body. It has the functions of maintaining the physiological function of the heart, increasing immunity, anti-oxidation, anti-cancer, detoxification and detoxification, and protecting the liver. At the same time, selenium also has a certain preventive effect on cataracts, cardiovascular diseases, Keshan disease, Kashin-Beck disease, arthritis and other diseases. However, selenium cannot be synthesized independently in humans and animals, and must be obtained from food. Plants are a key link in the natural selenium ecological cycle and the most direct source of selenium for humans and animals. Therefore, the production of selenium-enriched crops is very important to solve the problem of seleniu...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G5/30C05G1/00
CPCC05D9/00C05G3/00C05G5/37C05F11/08
Inventor 李霞韩枝烨
Owner JIANGSU ZHIFENG BIOLOGICAL TECH CO LTD
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