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Paenibacillus mucilaginosus high producing strain, and culturing method and use thereof

A technology of bacillus and high-yield strains, which is applied in the field of agricultural microorganisms, can solve the problems of poor spore-producing ability and weak silicon-decomposing ability of bacillus, and achieve the effect of increasing rice ear length and increasing yield per mu

Inactive Publication Date: 2015-04-01
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing jelly-like Paenibacillus has poor ability to desilicate and produce spores, which limits its application as a silicon fertilizer.

Method used

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  • Paenibacillus mucilaginosus high producing strain, and culturing method and use thereof
  • Paenibacillus mucilaginosus high producing strain, and culturing method and use thereof
  • Paenibacillus mucilaginosus high producing strain, and culturing method and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The acquisition of embodiment 1 jelly-like Paenibacillus high-yielding bacterial strain

[0023] 1. Prepare the cells of the type strain

[0024] Pick a single colony of the starting bacterium (Paenibacillus jelly-like bacillus ACCC01429), insert it into a simple medium containing 1% starch, 0.5% yeast extract, 0.5% sodium chloride, and pH7.2-7.5. Cultivate on a constant temperature shaker at 180rpm for 16-24h, then take 50ml of fermentation broth and centrifuge to collect the bacterial cells for use.

[0025] 2. N ion beam mutagenesis strong desilicon performance and screening of high-yielding spore-producing strains

[0026] Dissolve the bacteria prepared in step 1 with 5ml of sterile water and shake evenly. Take 0.5ml and apply it on a sterile petri dish and dry it. Use a TITAN ion beam implanter to perform N ion beam implantation on the bacteria with an energy of 30keV. The dose is 1 x 10 16 ion / cm 2 , after injection, wash the bacteria on the petri dish with 5m...

Embodiment 2

[0041] The cultivation of embodiment 2 bacterial strains

[0042] The culture method step of jelly-like Paenibacillus of the present invention is as follows:

[0043] 1) Strain activation: Inoculate the high-yielding Paenibacillus jelly-like strain with the preservation number CCTCC NO:M2014321 into the slant medium, and cultivate it at a temperature of 28°C for 24 hours;

[0044] The composition of the slant medium is as follows: 5-15% of sucrose, 0.03-2% of dipotassium hydrogen phosphate, 0.2-1.5% of silicon dioxide, 0.01-1% of magnesium sulfate, 0.05-0.5% of calcium carbonate, yeast extract 0.02-0.1%, agar powder 1.5-2%, pH7.2-7.4;

[0045] 2) Seed culture: put 50ml liquid seed culture medium in a 500ml Erlenmeyer flask, sterilize, then connect the activated strains obtained in step 1) to the seed culture medium, and cultivate at 32°C and 180rpm for 16-20 hours, the seed solution was obtained;

[0046] The composition of the seed medium is as follows: starch 1.5-2.5%, ye...

Embodiment 3

[0050] Embodiment 3 field application test

[0051] The field application test of Paenibacillus jelly-like with preservation number CCTCC NO:M2014321 was carried out on rice and melon as follows.

[0052] 1. Field application test of Paenibacillus jelly-like bacteria in rice

[0053] 1. Test field selection: The test field is a sandy mud field with medium soil texture and medium fertility. The previous stubble is rapeseed. Before the test, the soil analysis was carried out on the plow layer. The organic matter was 16.501g / kg, the alkali-hydrolyzed nitrogen was 85mg / kg, and the available phosphorus was 7.2mg. / kg, available potassium 76mg / kg, pH value is 5.2, and the test crop is hybrid rice Yangliangyou No. 6;

[0054] 2. Experimental design:

[0055] Treatment 1: conventional fertilization + jelly-like Paenibacillus CCTCC NO: M2014321 bacterial fertilizer;

[0056] Preparation of jelly-like Paenibacillus CCTCC NO:M2014321 bacterial fertilizer: mix mica powder with jelly-li...

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Abstract

The invention discloses a Paenibacillus mucilaginosus high producing strain KN-18. The Paenibacillus mucilaginosus high producing strain KN-18 is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO:M2014321. The strain has extremely strong silicon releasing and spore producing ability, and the silicon releasing ability of a strain broth can reach 26.8[mu]g / ml, and is substantially better than the silicon releasing ability of an original strain and other Paenibacillus mucilaginosus. The invention also discloses a culturing method of the Paenibacillus mucilaginosus high producing strain. The spore number of the broth obtained through the method can reach 2 billions / ml, and is substantially better than that of other culturing methods. The Paenibacillus mucilaginosus can be used in rice as a biological fertilizer to increase the stem height of the rice by above 5%, increase the length of spike of rice by above 4%, increase the number of filled grains by above 7% and the yield per 666.667m<2> by above 9%; and the Paenibacillus mucilaginosus can be used in muskmelon to make the muskmelon mature 2d earlier, increase the melon number per plant by 0.1, increase the weight per melon by above 5% and the yield per 666.667m<2> by above 9%.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms. More specifically, the invention relates to a high-yielding Paenibacillus jelly-like strain, a cultivation method of the strain and its use as a biological fertilizer. Background technique [0002] Insufficient silicon content in the soil is a key factor limiting crop yields. Silicon first condenses in the form of silica gel on the surface tissues of plant roots and leaves. The thickened surface increases the stability of plant structures and improves plant health. In addition, the precipitation of silicon in plant tissues also reduces evaporation, thereby reducing the impact of drought and salinity. Silicate bacteria can decompose silicon in soil and minerals, and provide sufficient silicon for plants. [0003] Paenibacillus jelly-like, also known as Bacillus jelly, is usually called silicate bacteria in China. It can decompose orthoclase and apatite to release phosphorus a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05D9/00C12R1/01
CPCC05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 余娜程治国刘华梅周莉唐纬坤邹伟杨克华王晓辉姚金伍顾志胜李青
Owner WUHAN KERNEL BIO-TECH CO LTD
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