Multiple species inoculant for enhancing sediment nutrient and preparation method thereof

A compound bacterial agent and nutrition technology, applied in the field of microorganisms, can solve the problems of insufficient matrix nutrition enhancement, insufficient Bacillus spore formation rate, affecting product quality and shelf life, etc. The effect of raising, increasing the number

Inactive Publication Date: 2016-07-27
SICHUAN GUOKE ZHONGNONG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention overcomes the deficiencies in the prior art, provides a compound microbial agent for enhancing matrix nutrition and its preparation method, and solves the problem that the existing microbial agent is not strong enough to enhance matrix nutrition, the bacterial content in the bacterial agent is insufficient, and the spores of Bacillus Insufficient yield rate, short survival time, seriously affecting product quality and shelf life

Method used

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  • Multiple species inoculant for enhancing sediment nutrient and preparation method thereof
  • Multiple species inoculant for enhancing sediment nutrient and preparation method thereof
  • Multiple species inoculant for enhancing sediment nutrient and preparation method thereof

Examples

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Effect test

Embodiment 1

[0032] A composite bacterial agent for improving substrate nutrition, said composite bacterial agent includes Azotobacter terroes, Bacillus megaterium and Paenibacillus jelly-like, the bacterium liquid of said Azotobacter rutatus, the bacterium liquid of Bacillus megaterium , The bacterium liquid volume ratio of jelly-like Paenibacillus is 2:1:1; The bacterium liquid bacterium content of described Azotobacter torus is 9.86 billion / ml, and the bacterium liquid bacterium content of described Bacillus megaterium is 4.7 100 million / ml, the bacterium liquid content of described colloid bacillus is 500 million / ml.

[0033] Wherein, the expansion culture method of the described Azotobacter toruferi strain is: put 120mlml of Azotobacter toruferus bacterial culture solution into a 500ml Erlenmeyer shaker flask, inoculate 7.5ml of the primary purified primary seed of Azotobacter toruferi, and then shake the flask , rotating shaker set speed 90-100rpm, culture 36h, culture temperature is...

Embodiment 2

[0038] A compound bacterial agent for improving matrix nutrition, said composite bacterial agent comprises Azotobacter terroes, Bacillus megaterium and Paenibacillus jelly-like, the bacterium liquid of said Azotobacter rutatus, the bacterium liquid of Bacillus megaterium , The bacterium liquid volume ratio of jelly-like Paenibacillus is 4:1:1; The bacterium liquid bacterium content of described Azotobacter torus is 8,000,000,000 / ml, and the bacterium liquid bacterium content of described Bacillus megaterium is 450,000,000 / ml, the bacterium liquid content of described colloid bacillus is 450,000,000 / ml.

[0039] Wherein, the expansion culture method of the described Azotobacter toruferi strain is: put 150ml of Azotobacter toruferus bacterial culture solution into a 500ml Erlenmeyer shaker flask, inoculate 7.5ml of the primary purified Azotobacter toruferus strain, and then shake the flask , rotating shaker set speed 90-100rpm, culture 40h, culture temperature is 31 ℃; then ino...

Embodiment 3

[0044] A composite bacterial agent for improving substrate nutrition, said composite bacterial agent includes Azotobacter terroes, Bacillus megaterium and Paenibacillus jelly-like, the bacterium liquid of said Azotobacter rutatus, the bacterium liquid of Bacillus megaterium , the bacterium liquid volume ratio of jelly-like Paenibacillus is 3:1:1; The bacterium liquid bacterium content of described Azotobacter torus is 10,000,000,000 / ml, and the bacterium liquid bacterium content of described Bacillus megaterium is 5 100 million / ml, and the bacterium liquid content of described colloid bacillus is 500 million / ml.

[0045] Wherein, the expanded culture method of the described Azotobacter toruferi strain is: put 135ml of Azotobacter toruferus bacterial culture solution into a 500ml Erlenmeyer shaker flask, inoculate 4.5ml of the primary purified primary seed of Azotobacter toruferi, and then shake the flask , rotating shaker set speed 90-100rpm, culture 38h, culture temperature i...

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Abstract

The invention discloses a multiple species inoculant for enhancing sediment nutrient. The multiple species inoculant comprises azotobacter chroococcum, bacillus megaterium and paenibacillus mucilaginosus; a volume ratio of bacteria solution of the azotobacter chroococcum to bacteria solution of the bacillus megaterium to bacteria solution of the paenibacillus mucilaginosus is 3:1:1. According to the invention, a segmented aerated culture method is adopted to carry out culture expansion, and the temperature is also changed with time of bacteria solution culture. The multiple species inoculant disclosed by the invention has the beneficial effects that the bacterial proportion of the multiple species inoculant can effectively enhance the sediment nutrient of an organic fertilizer, and the organic fertilizer can be decomposed to a great degree so as to enable crops to adsorb the organic fertilizer; compared with a multiple species inoculant which does not adopt segmented aerated culture, the multiple species inoculant adopting segmented aerated culture is more beneficial to bacterium breeding, enables a living bacteria count to be improved, and is beneficial for forming bacillus stearothermophilus spores; according to the multiple species inoculant disclosed by the invention, in different periods, different temperatures are adopted to culture bacillus, which enables the living bacteria count to be improved and is beneficial for forming spores.

Description

technical field [0001] The invention relates to the field of microbes, and more specifically relates to a composite bacterial agent for improving substrate nutrition and a preparation method thereof. Background technique [0002] Nitrogen-fixing bacteria are one of the very important functional groups in soil ecosystems and play an irreplaceable role in soil nitrogen cycle. Compared with chemical fertilizers, using it as an inoculant for microbial fertilizers has the characteristics of low cost, safe use, good continuous effect, stable production increase, less non-renewable energy consumption, environmental and food safety, and high economic benefits. Studies on the use of nitrogen-fixing bacteria as field inoculants have been widely carried out around the world and some gratifying results have been obtained. Many research results show that the inoculation of nitrogen-fixing bacteria can promote the growth of plants and is accompanied by a certain effect of increasing yiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C12R1/065C12R1/11C12R1/07
CPCC12N1/20C05F3/00C05F11/00C05F11/08
Inventor 李浩叶菁
Owner SICHUAN GUOKE ZHONGNONG BIO TECH CO LTD
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