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Bacterial strain for producing exopolysaccharides, method for producing exopolysaccharides by using bacterial strain and application of bacterial strain

A technology of exopolysaccharides and bacterial strains, which is applied in the field of Paenibacillus coliformis to produce exopolysaccharides, which can solve the problems of few reports on plant growth-promoting functions, and achieve the effect of improving stress resistance and promoting growth

Active Publication Date: 2021-07-23
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the functions of exopolysaccharides secreted by Paenibacillus coliformis mainly focus on improving its fermentation yield, sewage flocculation, egg and poultry breeding feed and moisturizing cosmetics, but its growth-promoting function on plants has rarely been reported.

Method used

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  • Bacterial strain for producing exopolysaccharides, method for producing exopolysaccharides by using bacterial strain and application of bacterial strain
  • Bacterial strain for producing exopolysaccharides, method for producing exopolysaccharides by using bacterial strain and application of bacterial strain
  • Bacterial strain for producing exopolysaccharides, method for producing exopolysaccharides by using bacterial strain and application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the method utilizing Paenibacillus colloidus to produce exopolysaccharide

[0034] Utilize the method for producing extracellular polysaccharide from Paenibacillus mucilaginosus G78, comprising the following steps:

[0035] S1. Activate the preserved Paenibacillus mucilaginosus G78 in a solid medium, then inoculate it in a seed liquid medium, and culture it on a shaker at 25-34°C and 160-200rpm for 8-16h to obtain a seed solution ;

[0036] S2. Transfer the seed solution in step S1 into the fermentation medium, and culture it on a shaker at 25-34° C. and 160-200 rpm for 75-80 hours to obtain a fermentation medium;

[0037] S3. Centrifuge the fermentation culture solution in step S2 at 4°C at 9000-12000rpm for 30-45min, remove the bacteria, add 3 times the volume of absolute ethanol to precipitate, leave it at room temperature overnight, collect exopolysaccharide, purify and prepare into dry powder.

[0038] Wherein, in the step S1, the solid medium comp...

Embodiment 2

[0044] Embodiment 2, exopolysaccharide component analysis

[0045] Experimental materials: Glucose (Glucose), mannose (Mannose), and glucuronic acid (Glucuronic acid) 3 standard products, and the exopolysaccharide (EPS) obtained in Example 1 was analyzed by GC-MS.

[0046] The result shows: see Figure 3-5 , multiple peaks were detected for each standard, and the numbers following the metabolites represent isomers, or reaction products of that substance. Three kinds of monosaccharides can be detected in EPS samples, namely glucose, mannose and glucuronic acid.

Embodiment 3

[0047] Embodiment 3, pot experiment

[0048] The plant nutrient solution used in the pot experiment includes the following mass concentrations of components:

[0049] Ca(NO 3 ) 2 4H 2 O 5mM; KNO 3 5mM; MgSO 4 ·7H 2 O 2mM; Fe-EDTA 20μM; H 3 BO 3 4.6 μM; CuSO 4 0.32μM; ZnSO 4 ·7H 2 O 0.77 μM; MnCl 2 11.1 μM; H 2 MoO 4 0.38μM, the pH value of the plant nutrient solution is 7.0.

[0050] The EPS used in this example is the exopolysaccharide obtained in Example 1.

[0051] 1. After the tomato seeds germinate, put them in the hole tray and cultivate them for 1 month, then select tomato seedlings of the same size and transfer them to flower pots for soil cultivation, and water them normally. Experimental treatment was carried out on the 3rd day after transplanting: the concentration of EPS was 50mg / L, and each seedling was irrigated with 20-50ml. Taking plants without EPS as a control, watering was performed once in 7 days, and watered 3 times in total. After tran...

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Abstract

The invention discloses a bacterial strain for producing exopolysaccharides, a method for producing the exopolysaccharides by using the bacterial strain and application of the strain, relates to the technical field of biology, and particularly discloses paenibacillus mucilaginosus, a method for producing the exopolysaccharides by using the paenibacillus mucilaginosus and application of the paenibacillus mucilaginosus. According to the invention, the preserved paenibacillus mucilaginosus is used for producing the exopolysaccharides, which can promote the growth of plants such as tomatoes and the like and improve the stress resistance of the plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Paenibacillus mucilaginosus, and a method and application for producing exopolysaccharides using the Paenibacillus mucilaginosus. Background technique [0002] In recent years, the unreasonable use of chemical fertilizers and pesticides in my country has not only seriously damaged the soil ecological environment, but also endangered food safety and affected people's physical and mental health. A large number of studies have found that microbial preparations can activate soil nutrients and are indispensable inputs to support "weight loss and drug reduction" and ensure the quality and safety of agricultural products. Paenibacillus mucilaginosus is a kind of Gram-negative, facultative anaerobic bacteria widely distributed in soil, plant rhizosphere and other environments. P. mucilaginosus can dissolve insoluble ores and compounds containing phosphorus and potassium, known as silicate...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A01N63/25A01P21/00C05F11/08C12R1/01
CPCC12P19/04C12N1/20A01N63/25C05F11/08
Inventor 王丹顾文杰卢钰升石超宏徐培智解开治孙杰孙丽丽黎婉玲
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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