Purification and breeding method of spirulina species
A technology of spirulina and algae species, applied in the direction of bacteria, etc., can solve the problems of low efficiency of pure breeding, difficulty in adapting, poor controllability, etc.
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[0013] A method for purifying and breeding spirulina species, comprising the following steps: (1) taking 3 L of spirulina algae culture fluid; (2) filtering the culture fluid; (3) filtering the filtered algae Transfer the mud into the triangular flask, add 200-300ml of medium L6, and carry out adaptive recovery culture. The control environment is light 4000lx and temperature 35°C; (4) Alkaline cleaning of the algae mud; (5) Cleaning of the algae mud ; (6) Take the upper layer of the liquid to suspend the algae, add 50-100ml medium L 6 , and mix well; (7) Dilute; (8) Take 1ml of liquid, transfer it into a 1-5ml sterilized centrifuge tube, place the centrifuge tube in a 2000lx 30°C water bath, and carry out adaptive recovery culture for 12 hours; ( 9) In the sterile clean bench, use a monofilament separator to select a single spirulina with large algal body and more spirals, and then expand the single spirulina with large algal body and more spirals to produce algal species in b...
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Abstract
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