Mode for forming nano-silver coating film on stem cell culture suction tube under assistance of magnetron sputtering and chemical gradient sedimentation
A stem cell culture, magnetron sputtering technology, used in sputtering plating, devices for coating liquids on surfaces, ion implantation plating, etc. Effectively activate and promote the growth of damaged tissue cells, and can not effectively protect from being eroded by external microorganisms, etc., to achieve strong antibacterial isolation, strong antibacterial shielding effect, and promote wound healing.
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Embodiment 1
[0056] The stem cell culture straw made of polyethylene oxide PEO material was disinfected and cleaned by high-frequency oscillation. The stem cell culture straw was placed as the base material into the silver target, and 860 coils of copper wire with a diameter of 0.1 cm were wound on the outer target outside the vacuum chamber by magnetron sputtering. Clean the vacuum chamber, place the base material (stem cell culture straw) on the inner target, and close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and input nitrogen filling into the vacuum chamber, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the substrate material (stem cell culture straw) through magnetron sputtering, and use the silver target to bombard the stem cells on the substrate Bombard the culture pipette for 20 minutes, close the silver target for natural cooling for 20 minutes, and then open t...
Embodiment 2
[0058] The stem cell culture straw made of polyethylene oxide PEO material was disinfected and cleaned by high-frequency oscillation. The stem cell culture straw was placed as the base material into the silver target, and 860 coils of copper wire with a diameter of 0.1 cm were wound on the outer target outside the vacuum chamber by magnetron sputtering. Clean the vacuum chamber, place the base material (stem cell culture straw) on the inner target, and close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and input nitrogen filling into the vacuum chamber, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the substrate material (stem cell culture straw) through magnetron sputtering, and use the silver target to bombard the stem cells on the substrate The culture pipette was bombarded for 15 minutes, and the silver target was closed for natural cooling for 26 minutes. ...
Embodiment 3
[0060] The PEO-like polymer stem cell culture straw is sterilized and cleaned by high-frequency vibration, and the stem cell culture straw is placed into the silver target. Through electromagnetic sputtering, bombard the target silver target, wind 860 turns of copper wire with a diameter of 0.1 cm on the outer target outside the magnetron sputtering vacuum chamber, clean the vacuum chamber, and place the base material (stem cell culture straw) on the inner target, Close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and fill the vacuum chamber with helium, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the base material (stem cell culture straw) by magnetron sputtering, and use the silver target to bombard the base material 20min, turn off the silver target and let it cool naturally for 20min. Then open the silver target to bombard the base material (stem cell c...
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