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Mode for forming nano-silver coating film on stem cell culture suction tube under assistance of magnetron sputtering and chemical gradient sedimentation

A stem cell culture, magnetron sputtering technology, used in sputtering plating, devices for coating liquids on surfaces, ion implantation plating, etc. Effectively activate and promote the growth of damaged tissue cells, and can not effectively protect from being eroded by external microorganisms, etc., to achieve strong antibacterial isolation, strong antibacterial shielding effect, and promote wound healing.

Inactive Publication Date: 2021-06-01
西安美丽起点生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Stem cell culture straws in the prior art are ordinary polymer materials, which cannot effectively protect stem cells from being corroded by external microorganisms, especially the erosive growth of bacteria during the process of culturing stem cells.
[0004] Moreover, ordinary polymer stem cell culture straws cannot improve the microcirculation of the tissue around the wound, and at the same time cannot effectively promote wound healing, rapidly promote the repair and regeneration of damaged cells, and fail to eliminate the inflammatory response
Can not effectively activate and promote the growth of damaged tissue cells, reduce scar formation, and accelerate wound healing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The stem cell culture straw made of polyethylene oxide PEO material was disinfected and cleaned by high-frequency oscillation. The stem cell culture straw was placed as the base material into the silver target, and 860 coils of copper wire with a diameter of 0.1 cm were wound on the outer target outside the vacuum chamber by magnetron sputtering. Clean the vacuum chamber, place the base material (stem cell culture straw) on the inner target, and close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and input nitrogen filling into the vacuum chamber, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the substrate material (stem cell culture straw) through magnetron sputtering, and use the silver target to bombard the stem cells on the substrate Bombard the culture pipette for 20 minutes, close the silver target for natural cooling for 20 minutes, and then open t...

Embodiment 2

[0058] The stem cell culture straw made of polyethylene oxide PEO material was disinfected and cleaned by high-frequency oscillation. The stem cell culture straw was placed as the base material into the silver target, and 860 coils of copper wire with a diameter of 0.1 cm were wound on the outer target outside the vacuum chamber by magnetron sputtering. Clean the vacuum chamber, place the base material (stem cell culture straw) on the inner target, and close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and input nitrogen filling into the vacuum chamber, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the substrate material (stem cell culture straw) through magnetron sputtering, and use the silver target to bombard the stem cells on the substrate The culture pipette was bombarded for 15 minutes, and the silver target was closed for natural cooling for 26 minutes. ...

Embodiment 3

[0060] The PEO-like polymer stem cell culture straw is sterilized and cleaned by high-frequency vibration, and the stem cell culture straw is placed into the silver target. Through electromagnetic sputtering, bombard the target silver target, wind 860 turns of copper wire with a diameter of 0.1 cm on the outer target outside the magnetron sputtering vacuum chamber, clean the vacuum chamber, and place the base material (stem cell culture straw) on the inner target, Close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and fill the vacuum chamber with helium, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the base material (stem cell culture straw) by magnetron sputtering, and use the silver target to bombard the base material 20min, turn off the silver target and let it cool naturally for 20min. Then open the silver target to bombard the base material (stem cell c...

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PUM

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Abstract

The invention discloses a mode for forming a nano-silver coating film on a stem cell culture suction tube under the assistance of magnetron sputtering and chemical gradient sedimentation, which comprises the following steps that a, the stem cell culture suction tube is disinfected and is cleaned through high-frequency oscillation; b, the stem cell culture suction tube is placed in a steady state, a three-dimensional maximum diameter under a horizontal condition is removed, a silver target is customized, and the stem cell culture suction tube is placed into the silver target; c, bombarding is conducted on the target object silver target through electromagnetic sputtering, a copper wire of 860 turns with the diameter of 0.1 cm is wound on an outer target on the outer side of a magnetron sputtering vacuum chamber, the vacuum chamber is cleaned, the substrate material (the stem cell culture suction tube) is placed in an inner target, the vacuum chamber is sealed and starts to be vacuumized with the vacuum degree being 10 <-6 >; and d, inert gas is input into the vacuum chamber for filling. The stem cell culture suction tube formed by the invention has the effects of killing bacteria, fungi, mycoplasma, chlamydia, spirochete and even trichomonad in clinical use, and does not generate drug resistance.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, biomedicine, nanotechnology and chemical gradient sedimentation, in particular to a method for forming a nano-silver coating on stem cell culture straws assisted by magnetron sputtering and chemical gradient sedimentation. Background technique [0002] Nanometer (nm) is the smallest unit of measurement after the micron in the physics world, with the development of nanotechnology. A large number of nanomaterials have been found to have antiphysical phenomena. As well as the quality and effect of special functions, nano-silver is also after the extensive development of metal nano-materials. an emerging field of research. Silver in the nanometer state has produced a qualitative leap in the bactericidal ability used in biomedicine. A very small amount of nano-silver can produce a powerful bactericidal effect that was unimaginable in the past. Nano-silver can kill more than 650 kinds of bacter...

Claims

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Application Information

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IPC IPC(8): C23C14/35C23C14/20B05D7/24B05D1/00
CPCB05D1/005B05D7/24C23C14/205C23C14/35
Inventor 刘哲
Owner 西安美丽起点生物科技有限公司
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