74 results about "TGF beta 1" patented technology

A pharmaceutical composition comprising a prostaglandin such as misoprostol and TGF-B in amounts sufficient to stimulate production of chondrocyte matrix is disclosed which exhibit therapeutic synergy.

The invention relates to a medicament for treating idiopathic pulmonary fibrosis. Dried ginger, schisandra, seed of snakegourd, cassia twig, bupleurum, pinellia, codonopsis pilosula, radix glycyrrhizae preparata, ginger and jujube are used as raw material medicaments and can be prepared into any common oral preparation. The medicament of the invention has the efficacy of removing evil diseases, conditioning cold and heat, relating lung qi and relieving cough and asthma, and can be used for treating the idiopathic pulmonary fibrosis. Animal test results show that the medicament of the invention can relieve abnormal change of PF I rat lung tissue, regulate free radical metabolism in a model body of rat pulmonary fibrosis, enhance the activity of GSH-Px and S0D, reduce the content of MDA and NO and effectively reduce PDGF, TGF-beta 1 and TNF-a levels in blood serum, and has the functions of preventing and treating pulmonary damage and fibrosis caused by bleomycin. Clinical tests prove that the total effective rate of the medicament for treating the idiopathic pulmonary fibrosis is up to 88.5%.

The invention relates to a drug application of a chemical substance wedelolactone for treatment of pulmonary fibrosis. The chemical substance wedelolactone is derived from a traditional Chinese medicine eclipta, and the structure is determined through spectral data; the wedelolactone can significantly improve the degree of model mouse lung tissue pulmonary fibrosis after intragastric administration, reduce NO (Nitrogen Oxide) and MDA (Methylene dioxyamphetamine) contents reflecting degree of lung injury, HYP (Hydroxyproline) content reflecting collagen deposition in the lung tissues, and cell factor TGF-beta 1 (Transforming Growth Factor) content causing pulmonary fibrosis, and inhibit human embryo lung fibroblast proliferation in vitro and HYP content of the human embryo lung fibroblast. In-vivo and in-vitro experiments show that wedelolactone can obviously improve bleomycin induced mouse pulmonary fibrosis, so that the wedelolactone can be taken as a novel drug for treating pulmonary fibrosis.

The invention provides a defined low protein culture medium for maintaining cells in an undifferentiated state, the medium comprising: a basal medium, an organic acid from the tricarboxylic acid cycle, nonessential amino acids, a combination of growth factors selected from the group consisting of FGF-2 protein, an IGF-1 protein or insulin, a Transferrin protein, and a TGF beta 1 protein, wherein the medium is essentially feeder-free, essentially xeno-free, essentially free of beta-mercaptoethanol, and essentially free of animal-derived or human-derived proteins.

The invention relates to application of captopril to the preparation of medicaments for treating and/or inhibiting scar hyperplastic diseases. Particularly, the hyperplastic diseases comprise hyperplastic scar, cheloid, urethral scar stricture and silicon gel breast augmentation prosthesis postoperative capsular contracture. When the diseases are treated, the captopril is used as an active ingredient, clinically-used emulsifiable paste, gel, injection and the like in different formulations are prepared by the conventional preparation process. The study indicates that the captopril can reduce the capacity of synthesizing pathologic scar fibroblast collagen and secreting the collagen, weaken the multiplication capacity of fibroblasts and reduce the expression of transforming growth factor-beta 1 (TGF-beta 1), and has a good treatment or prevention effect on pathologic scars. Simultaneously, the captopril acts on scar tissue directly, has the advantages of small dose, high curative effect, small side effect, mature raw material process, readily available materials, low cost and the like, and is expected to become a main medicament for treating and preventing the scars clinically.

The invention discloses a kit for screening colorectal cancer hereditary susceptibility genes. The kit comprises colorectal cancer susceptibility gene specificity primers for amplifying a plurality of target regions in a to-be-detected sample, wherein the colorectal cancer susceptibility genes include at least one of rs10795668, MMP2, SMAD7, ADH2, ALDH2, CYP1A2, MMP-1, MTHFR, TP53, VEGF, COX-2, DNMT3B, hMLH1, LOC727677, MMP9, MTRR and TGF-beta 1. The kit can detect a plurality of regions of the colorectal cancer susceptibility genes at the same time, detecting efficiency is improved, detecting cost is reduced, and detecting accuracy and sensitivity are high.

The invention discloses application of autovaccine preparation containing TGF beta 1. Adjuvant is added in the autovaccine preparation to prepare medicinal preparation for treating continuous pathogen infection of livestock. The vaccine preparation is fusion protein obtained by the way that part or total of the TGF beta 1 or part or total of mutant or total of similar gene sequences is recombined with at least one helper T cell capable of improving immunogenicity or the gene sequence of carrier protein to construct eukaryon or pronucleus expression plasmid and transform host cell for expression, or is directly prepared by eukaryon expression plasmid obtained by construction. The vaccine preparation can be directly used for preparing medicinal preparation for treating the continuous pathogen infection of livestock, induce domestic animals to generate antibody capable of neutralizing self TGF beta 1, and remove immunodepression function caused by too high level of TGF beta 1 in continuous process of infection; simultaneously, the vaccine preparation can depress the generation of inducible regulatory T cell at the persistent infection part of an organism, thus being beneficial to breaking the immune tolerance state of in-vivo pathogen by the organism and promoting the removing function of the organism on the continuously infectious pathogen.

The invention discloses a betulin derivative as well as a preparation method and application thereof. The structural formula of the betulin derivative provided by the invention is shown as a formula I, a formula II, a formula III, a formula IV, a formula V, a formula VI or a formula VII. According to the invention, the betulin is successfully subjected to structural modification by utilizing a microbial conversion technology, so that a plurality of novel compounds are obtained. In vitro TGF-beta 1 induced HK-2 cell experiments prove that the compounds have a relatively good protection effect on HK-2 cell damage. The compounds can be used as active ingredients of a medicine for preventing or treating nephropathy and have wide application.

Methods and devices are described for the regulation of BMP 2 and 4, TGF-beta 1, 2, and 3, FGF-2, osteocalcin, and alkaline phosphatase mRNA in stem cells via capacitive coupling or inductive coupling of specific and selective electric and/or electromagnetic fields to the bone cells or other tissues containing the stem cells, where the specific and selective fields are generated by application of specific and selective signals to field generating devices disposed with respect to the stem cells so as to facilitate the treatment of diseased or injured bone and other tissues. The resulting methods and devices are useful for the targeted treatment of osteoporosis, osteopenia, osteonecrosis, fresh bone fractures, fractures at risk, nonunion, bone defects, spine fusion, and/or other conditions in which BMP 2 and 4, TGF-beta 1, 2, and 3, FGF-2, osteocalcin, and alkaline phosphatase mRNA and/or protein deficiencies in stem cells has been implicated.

The invention relates to the technical field of medicine, in particular to the application of astragalus extract to preparation of NF-kappa B inhibitor. Experiments prove that the astragalus extract can inhibit chronic nephritis NF-kappa B expression and further affect the level of IL-6, TNF-alpha, TGF-beta 1 and MCP-1 and reduce proteinuria. Therefore, the astragalus extract can serve as an active component for inhibiting chronic kidney disease NF-kappa B and be used for preparing drugs for treating chronic kidney disease.

The invention discloses application of saikoside d in preparation of anti-hepatic fibrosis disease drugs, and belongs to the technical field of medicines. The invention aims to provide pharmacological activities exhibited by the saikoside d in treatment of hepatic fibrosis symptoms and related mechanisms. The saikoside d has a good anti-hepatic fibrosis effect, and the action mechanisms may be related with improvement of activities of superoxide dismutase, reduction of generation of oxyradical and inhibition of activation of TGF-beta 1 (transforming growth factor) signaling pathway.

The invention belongs to biotechnology field and particularly relates to high throughput screening of an effective anti-hepatic fibrosis compound by means of picric acid-sirius red staining method on a collagenous fiber. The cell is hepatic stellate cell line CFSC-8B, transforming growth factor TGF-beta1 with the dose of 10ng/mL can obviously induce the CFSC-8B cell to secrete collagen when acting for 48 hours, and the secretion of the CFSC-8B cell collagen induced by the TGF-beta 1 can be effectively detected by means of picric acid-sirius red staining method. The picric acid-sirius red staining method on the collagenous fiber is applied to screening the compounds with anti-hepatic fibrosis activities in different polarity extracts of antrodia camphorata, ganoderma lucidum, cephalosporium sinensis, cordyceps mortierella, armillaria and hericium erinaceus powder. Tests prove that n-hexane and chloroform extract of the antrodia camphorata, ethyl acetate extract from the cordyceps mortierella, and n-hexane, chloroform extract and ethyl acetate extract from the armillaria can effectively inhibit the secretion of the CFSC-8B cell collagen induced by the TGF-beta 1, the above extracts have dose dependent relationship, are significant in difference, and have an anti-hepatic fibrosis function, and other extracts do not have significant inhibition effect.

The invention relates to application of a chemical substance Eupatilin for treating pulmonary fibrosis. The compound Eupatilin is obtained from traditional Chinese medicine Folium Artemisiae Argyi, the structure of the Eupatilin is determined according to spectrum data, the fibrosis degree of lung tissues of model mice is improved obviously after intragastric administration of the Eupatilin, NO content and MDA content which reflect lung damage degree are reduced, HYP content reflecting lung tissue collagen deposition is lowered, and the content of cell factor TGF-beta 1 causing the pulmonary fibrosis is decreased; human embryonic lung fibroblast proliferation and HYP content are inhibited in vitro. In-vivo and in-vitro experiments show that the Eupatilin can remarkably improve bleomycin induced pulmonary fibrosis of the mice, and can be applied to drugs for treating the pulmonary fibrosis.