The invention discloses an application of a microgroove forming mold in preparation of silicone rubber with a microgroove pattern on the surface. The microgroove forming mold comprises a female mold, wherein microcosmic bulges used for microgroove forming and arranged in rows or lines are arranged at the bottom of the female mold, the microcosmic bulges are distributed according to the rules that the peak value ranges from 4 mu m to 6 mu m and the period ranges from 5 mu m to 7 mu m, and the depth of the female mold ranges from 0.4 mm to 0.6 mm. The silicone rubber surface is modified by the microgroove forming mold into a structure with the microgroove pattern, the water contact angle of the silicone rubber is increased, the hydrophilicity of the silicone rubber is reduced, growth behaviors of cells on the silicone rubber surface are further affected, the growth direction and space distribution of the cells on the material surface are further affected, finally, the incidence of capsular contracture is reduced, and a novel solution is provided for preparation of implant materials more applicable to clinical application.