67 results about "Plant secondary metabolism" patented technology

Methods for screening mutations that affect the synthesis of plant small molecules or compounds capable of activating a mammalian nuclear receptor protein and systems for rapidly assigning functionality to genes that regulate plant secondary metabolism are provided.

The invention relates to an algaecide. The algaecide which can be used for effectively removing algae and precipitating alga bodies contains chitosan, fly ash autoclaved brick powder and allelopathic chemicals of gallic acid, nonoic acid or N-phenyl-naphthylamine, and is obtained by dissolving a certain amount of chitosan by using hydrochloric acid, then adding the fly ash autoclaved brick powder of 40-200 meshes, and then adding the dimethyl sulfoxide assisted dissolved allelopathic chemicals of gallic acid, nonoic acid, N-phenyl-1-naphthylamine or N-phenyl-2-naphthylamine. The chitosan and the fly ash autoclaved brick powder used in the algaecide provided by the invention are nontoxic, the allelopathic chemicals of gallic acid, nonoic acid and the like belong to plant secondary metabolism products, can be degraded easily in a water body and cannot cause secondary pollution, and although the diluted HCl solution is used for dissolving chitosan, great changes of the pH value of the water body are not caused in a dosage range. The algaecide provided by the invention can be used for removing the algae effectively and ensuring that the alga bodies are sunk to the bottom of water, has a small influence on the environment and does not cause secondary pollution, the water body is clear, and the beautiful outlook of the water body is not influenced, so that the algaecide is suitable for removing the algae from a small landscape water body taking blue algae as a dominant species.

The invention discloses a method for continuously obtaining plant secondary metabolites through tissue culture. The steps of the method include (1) obtaining plant bodies in batches; (2) producing and inducing plant secondary metabolites; and (3) plant secondary metabolites Collection and extraction of raw metabolites. The present invention also relates to a reactor device for cultivation. The upper part of the device adopts the layered culture design of plant tissues and organs, and the harvesting operation of the culture can be conveniently carried out in stages through the design of the multi-layered culture grid; Body design, its liquid storage tank can be easily disassembled, the desired product can be obtained by collecting the culture medium, and the inhibition of the secondary metabolite concentration on its continued production can be reduced through the replacement of the culture medium, so as to ensure the continuous acquisition of secondary metabolites. The invention has the advantages of large throughput, convenient operation and low cost.

The invention provides a method for producing beta-amyrin with a saccharomyces cerevisiae engineering bacterium, and belongs to the field of biochemical engineering. According to the method, a 2,3-oxidized squalene monooxygenase gene is cloned from candida albicans, a glycyrrhiza glabra beta-amyrin synthase gene subjected to codon optimization is chemically synthesized, a gene expression cassette is constructed by utilizing a saccharomyces cerevisiae constitutive promoter, the gene expression cassette and a plasmid after double digestion jointly invert saccharomyces cerevisiae, and assembly of the gene expression cassette and the plasmid is achieved by a homologous recombination function of the saccharomyces cerevisiae, so that 2,3-oxidized squalene monooxygenase is reinforced, and beta-amyrin synthase is led in. Saccharomyces cerevisiae engineering bacterium metabolizable glucose directly synthesizes beta-amyrin, and synthesis of beta-amyrin is coupled with growth of the saccharomyces cerevisiae, so that artificial synthesis of the saccharomyces cerevisiae of a plant secondary metabolism product, namely beta-amyrin is achieved. The method requires no effect agent induction, is simple in technology, and can be used for fermenting and producing beta-amyrin.