Large-scale metabolomequalitationmethod
A qualitative method and metabolome technology, applied in the field of analytical chemistry, can solve the problem of lack of scale, and achieve the effect of large-scale qualitative
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[0029] Taking plant leaves as an example, a large-scale qualitative study of its metabolites was carried out. The specific steps are: prepare a mixed plant sample, and perform ultra-high performance liquid chromatography-high resolution mass spectrometry analysis on the sample under different fragmentation voltages in the mass spectrometer source; obtain the peak table after peak matching of the original data, and obtain a characteristic from the peak table. For metabolites with neutral loss or characteristic product ions, search the mass spectrometry database to quickly lock the metabolite category and achieve large-scale characterization. For substances with commercially available standard samples, further standard sample verification can be carried out to confirm the qualitative results. Metabolites with specific neutral loss characteristics or characteristic product ions can use a single neutral loss, multiple neutral losses or neutral losses combined with multi-level mass...
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