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33results about How to "Retain enzyme activity" patented technology

Magnetic composite microsphere immobilized laccase and preparation method thereof

InactiveCN102206624AAvoid chemical damageFacilitate recycling and reuseOn/in inorganic carrierChemistryCarbonyl group
The invention relates to a magnetic composite microsphere immobilized laccase and a preparation method thereof, and belongs to the field of environmental microbiology. In the immobilized laccase, metal-chelated magnetic composite microspheres are taken as a carrier, and laccase is immobilized on the carrier through coordination bonding action. The preparation method comprises two steps of: preparing the carrier, namely synthesizing magnetic ferroferric oxide nanoparticles by using a chemical precipitation process, preparing core-shell magnetic silica by taking the nanoparticles as cores through a sol-gel method, grafting polymer containing carbonyl or amino on the surface of the magnetic silica by taking gamma-chloropropyltrimethoxysilane as a medium, and preparing a functional carrier capable of immobilizing the laccase by chelating transition metal ions (such as Cu2+. Zn2+, Ni2+ or Co2+); and immobilizing the laccase, namely performing coordination adsorption of the laccase on the surface of the carrier, washing, and drying to obtain the finished product of immobilized laccase. The immobilized laccase prepared by the method has high stability and activity and quick magnetic response, is easily recovered from a reaction system and can be repeatedly used; and the preparation process is simple and the cost is low.
Owner:BEIJING NORMAL UNIVERSITY

Mixed enzyme production process by solid fermentation of fruits and vegetables and microorganisms

InactiveCN104839638ARetain enzyme activityRetain non-enzyme active ingredientsFood preparationEnvironmental resistanceMicroorganism
The present invention discloses a mixed enzyme production process by solid fermentation of fruits and vegetables and microorganisms and the production process includes the following steps: step one, washing and cleaning fresh fruits and vegetables; step two, juicing the cleaned fruits and vegetables using a juice extractor and obtaining the fruit and vegetable juice and the fruit and vegetable residue; step three, adding water content adjusting raw materials according to different water content of the obtained fruit and vegetable residue to adjust the water content of the fruit and vegetable residue, and mixing the above materials evenly to obtain a base material; step four, putting the step three obtained base material into a fermentation bottle to conduct sterilization; step five, cooling the sterilized fruit and vegetable base material to room temperature, and inoculating microorganism liquid strains to conduct solid fermentation; and step six, after the end of solid fermentation, blending the obtained fruit and vegetable juice into the solid fermented materials, conducting low temperature drying, and thereby obtaining the fruit and vegetable and microorganism mixed enzymes containing both fruit and vegetable enzymes and microbial enzymes. The mixed enzymes can not only fully preserve the enzyme activity of fruits and vegetables, but also obtain the enzyme ingredients of microorganisms, and in addition, the production process can also achieve the full advantage of the entire fruits and vegetables, does not produce any waste, and is ecological and environmental protective.
Owner:杭州德润全健康产业发展有限公司

Black garlic as well as fermentation process thereof

The invention relates to the technical field of the food deep processing and particularly relates to black garlic as well as a fermentation process thereof. The fermentation process of the black garlic comprises the following steps of freezing fresh garlic sections at a freezing temperature being -10 DEG C to -40 DEG C for less than 48 hours; and fermenting through two stages, wherein constant-temperature fermentation is carried out for 2-10 days at the first-stage fermentation temperature being 10 DEG C-50 DEG C, and the constant-temperature fermentation is carried out for 4-12 days at the second-stage fermentation temperature being 70 DEG C-90 DEG C. According to the fermentation process disclosed by the invention, the content of substances such as reducing sugar, total phenols and amino acid in the garlic can be greatly increased without adding any additive, and the black garlic fermentation period is shortened, so that the whole fermentation process can be completed in only 8-14 days; the obtained black garlic has characteristics of being good in flavor, elastic in texture, high in nutritional ingredient, high in safety, easy to store, and the like.
Owner:QINGDAO AGRI UNIV

Fast-mellowing processing process of high-quality dark tea

The invention relates to a processing process of dark tea, in particular to a fast-mellowing processing process of high-quality dark tea. The processing process includes: picking fresh tea leaves; thinly spreading the fresh tea leaves; deactivating enzymes to completely deactivate the enzymes of the tea leaves and keep part of enzymatic activity of tender stems; spreading out for cooling, and rolling; performing pile fermentation; drying. The processing process has the advantages that dark tea mellowing is achieved and pile fermentation time is shortened, the half enzyme deactivating technology is used, part of the enzymatic activity of the tea stems is kept, cells are crushed through the rolling to release enzymes and substrates, the oxidation of polyphenols is catalyzed fast during the pile fermentation, and the forming of the high-quality dark tea; the mellowness of the dark tea can be improved effectively, the taste of the dark tea is improved evidently, the pile fermentation process is accelerated, dark tea processing time is shortened, and dark tea production efficiency is increased.
Owner:湖南省茶叶研究所

Lactobacillus plantarum and application thereof in fruit enzyme product

The invention discloses lactobacillus plantarum SITCC No.10011. The preservation number of the lactobacillus plantarum SITCC No.10011 is CTCC M 2017366; the removing rates of the lactobacillus plantarum SITCC No.10011 on DPPH free radicals in intracellular organic matters and metabolic products are respectively 3.5 percent and 35.8 percent. The lactobacillus plantarum CCTCC M 2017366 is used for preparing fruit enzyme products, so that a result shows that the DPPH free radical removing rate of the fruit enzyme products fermented by the lactobacillus plantarum SITCC No.10011 is improved by at least 40 percent compared with that of the naturally fruit enzyme products; therefore, the lactobacillus plantarum SITCC No.10011 has a very good application prospect in the fruit enzyme products.
Owner:河北麦克斯生物科技有限公司

Preparation method of health-care grapefruit enzyme drink

The invention provides a method for preparing a health-care enzyme drink from grapefruits and belongs to the technical field of bioengineering technologies. According to the method, the grapefruits are taken as a main fermentation raw material, are peeled and mashed to be pulpy, raw honey and purified water are added, nectariferous bacteria, lactobacillus casei and lactobacillus plantarum strains are taken as fermentation strains, a fermentation liquid which is 1%-5% of the volume of a fermentation stock solution is added to a fermentation system, the fermentation temperature is 25-35 DEG C, pH ranges from 5.0 to 8.0, the fermentation time lasts for about 10d, and the health-care grapefruit enzyme drink is prepared. According to the method, complete fermentation is realized, various enzymes, vitamins, amino acid and micro elements are sufficiently released, the product has a unique flavor of grapefruits, is palatably sweet and sour and has rich nutrition, and a traditional fermentation technique is adopted.
Owner:JIANGXI GUOGUO BIOTECH CO LTD

Probiotic enzymatic composition capable of regulating intestinal tract and application thereof

The embodiment of the invention discloses a probiotic enzymatic composition capable of regulating intestinal tract. The composition is prepared by mixing the following raw materials in percentage by weight: 65 to 75 percent of dietary fiber, 10 to 15 percent of comprehensive vegetable and fruit enzymatic powder, 10 to 15 percent of soybean protein powder, 2.5 to 4.5 percent of probiotic bacteria,1 to 2 percent of plant protease, 0.3 to 0.8 percent of soybean phospholipid, 0.5 to 0.8 percent of an acidifier and 0.08 to 0.15 percent of a sweetener. The probiotic enzymatic composition provided by the invention has a remarkable curative effect in regulating intestinal problems, can effectively prevent dyspepsia and improve constipation, and has the functions of protecting intestinal mucosa and enhancing immunity, thereby fundamentally solving intestinal problems. The composition provided by the invention is natural, free from side effects, and safe and effective in use, and has a broad market application prospect.
Owner:广州乔媛商贸有限公司

A porcine pancreatic lipase-inorganic hybrid nanoflower catalyst, a preparing method thereof, and applications of the catalyst in biodiesel preparation

InactiveCN107586770ARetain enzyme activityImprove enzyme toleranceHydrolasesBiofuelsNano catalystBiodiesel
The invention discloses a porcine pancreatic lipase-inorganic hybrid nanoflower catalyst, a preparing method thereof, and applications of the catalyst in biodiesel preparation, and belongs to the technical field of biological energy. The method includes adding 1-5 mL of an aqueous CuSO4 solution having a concentration of 80-300 mM into 100 mL of a PBS buffer solution the pH of which is 6-8 and into which 10-250 mg of porcine pancreatic lipase is dissolved; performing incubation at 0-30 DEG C for 24-72 h; then performing centrifugal separation; washing an centrifugation product with deionized water for 3-5 times; freeze-drying the product to obtain the catalyst; and storing the catalyst at -20 DGE C. The weight percentage of the porcine pancreatic lipase in the catalyst is 12-31%. Accordingto the method, no organic agent is used in a nanometer catalyst preparing stage, thus maintaining enzyme activity well and improving enzyme tolerance. In addition, the novel catalyst can avoid a complex separation process in biodiesel synthesis, and has a good reutilization efficiency.
Owner:JILIN UNIV

Arginine deiminase mutant from arthritis-type mycoplasma and application thereof

The invention discloses an arginine deiminase mutant from an arthritis-type mycoplasma, a gene, a polyethylene glycol modified substance and an application thereof. The arginine deiminase mutant is a dipolymer which is formed by two protein subunits, wherein amino sequences of the protein subunits are represented as SEQ ID No.2 in a sequence table. The arginine deiminase mutant is increased in expression quantity in escherichia coli, is increased in renaturation efficiency, is not changed in enzyme activity and is suitable for industrial production. The polyethylene glycol modified substance of the arginine deiminase mutant can be used for preparing anti-tumor drugs, such as anti-hepatoma drugs, anti-leukemia drugs and anti-melanoma drugs.
Owner:SHANGHAI INST OF PHARMA IND +1

Preparing method for polygonum perfoliatum leaf enzyme healthcare beverage

The invention provides a method for preparing an enzyme beverage from polygonum perfoliatum leaves. The method includes the steps that the polygonum perfoliatum leaves are used as a main fermentation raw material, raw honey and natural springwater provided by the Wuxi hundred-year Shangshui drinks limited company are added, nectar source bacterium, lactobacillus casei and lactobacillus plantarum strains are adopted as fermenting strains, fermenting bacterium liquid is added into a fermentation system, wherein the volume of the fermenting bacterium liquid accounts for 1-5% of the volume of a fermenting stock solution, and fermentation is carried out for about 10 d at the temperature of 25 DEG C-35 DEG C and the pH value of 5.0-8.0 to prepare the polygonum perfoliatum leaf enzyme healthcare beverage. Fermentation of the beverage is complete, various enzymes, vitamins, amino acid and microelement are fully released, and the beverage has the unique flavor of the polygonum perfoliatum leaves, is rich in nutrition and tasty and belongs to the technical field of bioengineering by the adoption of a traditional fermentation technology.
Owner:JIANGNAN UNIV

Manganese dioxide-based nano-drug carrier and preparation method and application thereof

The invention relates to a manganese dioxide-based nano-drug carrier, and innovatively combines hunger therapy and gas therapy for cancer treatment. Specifically, folic acid targeting molecules are introduced into human serum protein firstly by utilizing an amidation reaction, and then a nano manganese dioxide carrier is cultured in the protein by utilizing a biomineralization method. Glucose oxidase and L-arginine are loaded at the same time through physical adsorption. The preparation method has the advantages that 1, the targeted drug delivery efficiency and dispersity of the nano manganesedioxide are greatly improved, 2, specific chemical reaction in cancer cells is utilized to consume endogenous glucose and release nitric oxide gas to kill the cancer cells through hunger and gas therapy, 3, the synthesized carrier substance is degradable, and the loaded enzyme and amino acid are endogenous substances of a human body and have no toxic or side effect on the human body, and 4, the anticancer effect is remarkable, and cancer cells can be killed under extremely low biological enzyme concentration.
Owner:QUFU NORMAL UNIV

Method for preparing super paramagnetic carrier of cross-linking immobilized lipase

ActiveCN105037596AGood magnetic separation performanceOvercoming lipase activity-dependent interface effectsOn/in organic carrierMicrosphereMagnetic separation
The invention discloses a method for preparing a super paramagnetic carrier of a cross-linking immobilized lipase. The preparation of the super paramagnetic carrier is realized by preparing a nano-magnetic microsphere Fe3O4, coating the nano-magnetic microsphere Fe3O4, functionally modifying the coated nano-magnetic microsphere Fe3O4. A thermo-sensitive material, namely N-isopropylacrylamide, is introduced into the super paramagnetic carrier prepared according to the method, and the structural performance of the carrier can be changed through temperature control, so that the lipase which is immobilized through the super paramagnetic carrier can get out of the interface performance control to realize direct reactions among alcohols, acids and lipids, and separation of the reaction products is facilitated; moreover, lipase activity control through alpha coverage is facilitated. The super paramagnetic carrier is a super paramagnetic polymer microsphere based on the mode of lipase interface activity action; the lipase can be immobilized through the super paramagnetic polymer microsphere; the immobilized lipase is high in magnetic separation performance; 90% or higher of the lipase activity is kept; the detect that the lipase activity in an enzymatic reaction depends on the interface effect is overcome.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Maca concentrate, abelmoschus manihot and active rhizoma polygonati combined food and preparation method thereof

The invention provides a maca concentrate, abelmoschus manihot and active rhizoma polygonati combined food and a preparation method thereof. The preparation method comprises the following steps: 1, smashing maca concentrate until the granularity is 300 meshes; 2, smashing active abelmoschus manihot until the granularity is 300 meshes; 3, smashing active rhizoma polygonati until the granularity is 300 meshes; 4, mixing and pelletizing maca concentrate powder which accounts for 60-70% of the total weight, the active abelmoschus manihot powder which accounts for 15-20% of the total weight and the active rhizoma polygonati powder which accounts for 15-20% of the total weight at a set normal temperature of 15-30 DEG C, and tabletting at a set pressure of 10kg / g-15kg / g, thereby obtaining the maca concentrate, abelmoschus manihot and active rhizoma polygonati combined food. According to the preparation method, the active abelmoschus manihot and the active rhizoma polygonati which are obtained by an ultralow-temperature freeze drying method are adopted, all active ingredients of the abelmoschus manihot and the rhizoma polygonati are preserved, especially active enzymes and relevant enzymein the abelmoschus manihot and the rhizoma polygonati are preserved. The preparation method is simple, low in cost and convenient to operate.
Owner:百丰生物科技(武汉)有限公司

Method for producing pelargonium hortorum bailey skin care enzyme by utilizing honey source bacteria

The invention provides a method for making geranium skin care enzymes with nectar-derived bacteria. The technological feature is to use geranium as the main raw material, and the preservation number preserved in the China Typical Culture Collection Center on June 24, 2013 is: CCTCC NO: M2013283 Bacillus amyloliquefaciens H47 was the fermentation strain of Bacillus amyloliquefaciens. According to the volume ratio of geranium: raw honey: pure water is 2‑5: 1‑3: 7‑2, add it and mix it evenly to make a geranium fermentation stock solution, add the nectar source bacteria seed liquid to the fermentation stock solution according to the ratio of 1‑5%. After fermenting for 6-14 days at a temperature of 25°C-35°C and a pH value of 5.0-8.0, the crude product of geranium skin care enzyme can be obtained. Preliminarily screen out large particle residues through a coarse filter with a number of 20-32 meshes, and then further filter out small particle residues through a fine filter with a number of 80-100 meshes to obtain the geranium skin care enzyme.
Owner:JIANGNAN UNIV

Preparation method and application of nanogel with hunger combined gas therapy anti-tumor function

The invention discloses a preparation method and application of nanogel with a hunger combined gas therapy anti-tumor function. The nanogel is prepared by the following steps: firstly, performing electrostatic interaction on polyethyleneimine azide grafted with L-arginine and glucose oxidase to form a nano-enzyme compound; and then covering the surface of the nano-enzyme compound with an alkynyl-containing polycarbonate block copolymer, and performing click chemical crosslinking on azide and alkynyl. Compared with the prior art, according to the method disclosed by the invention, specific enzymatic reaction is utilized to consume endogenous glucose and release nitric oxide gas, cancer cells are killed through hunger and gas therapy, besides, the nitric oxide gas can redistribute oxygen in the cancer cells, hypoxia of tumors is relieved, and wide application prospects are realized in the aspects of resisting tumors and inhibiting tumor metastasis.
Owner:CHINA PHARM UNIV

Preparation method of graphene immobilized laccase

The invention provides a preparation method of graphene immobilized laccase. The graphene immobilized laccase is prepared by immobilizing laccase to a carrier of two-dimensional crystal material having excellent conductivity through nonspecific adsorbing action. The preparation method comprises the steps of preparing the carrier and immobilizing laccase; particularly, the carrier, graphene, is prepared by using a phenol as a raw material to carry out graphitization on sandwiched FePO4 / dodecylamine nanolayer composite, and stripping before synthesis; the immobilized laccase is immobilized by adsorbing laccase to the surface of the evenly dispersed graphene in nonspecific manner, centrifuging, washing, and drying to obtain the finished immobilized laccase. The carrier of graphene herein helps increase electron transfer rate of enzymatic catalytic reaction process and improve reaction efficiency; the immobilized laccase prepared via the preparation method has high fixing quantity, high activity and good stability, can be recycled from the reaction system, and is reusable; the preparation process is simple and feasible.
Owner:NANJING UNIV +1

Heat treatment clarification method of industrial enzyme liquid

The invention provides a heat treatment clarification method of an industrial enzyme liquid. The method comprises the following steps: (1) target protein selection, culture and fermentation: selectinga target protein, fermenting the protein according to the existing fermentation production process to obtain a fermentation liquid, and carrying out cell disruption treatment on the fermentation liquid to obtain a cell disruption liquid, (2) heat treatment clarification: carrying out heat treatment clarification on the cell disruption liquid obtained in the step (1), and (3) post-treatment: carrying out centrifugal treatment on the liquid subjected to heat treatment clarification in the step (2), and collecting the clear liquid to obtain the industrial enzyme clarified liquid of the target protein. The method has the advantages of simple process, low cost and environmental protection, the enzyme activity loss of the target protein in the clarified enzyme liquid is not more than 10%, the turbidity is lower than 500NTU, and the clarified enzyme liquid can pass through 0.2 micromoles per filter of a filtering membrane.
Owner:ENZYMASTER NINGBO BIO ENG CO LTD

Preparation method of Chinese wolfberry enzyme

The invention discloses a preparation method of a Chinese wolfberry enzyme, which is characterized by comprising the following steps: cleaning Chinese wolfberry, and drying to constant weight; grinding spina date seeds, albizia flowers, poria cocos, platycladi seeds and polygala tenuifolia into powder, and mixing; adding the Chinese wolfberry into a solvent, adding sugar, ascorbic acid, tea waterand the mixed powder to prepare a fermentation medium, sterilizing, inoculating a fermentation strain, and fermenting for 5-30 days; filtering the obtained fermentation liquor to remove residues, andcollecting supernatant, so as to obtain the Chinese wolfberry enzyme. The Chinese wolfberry enzyme prepared by the invention is free of chemical additives, improves the fermentation efficiency and thecontent of functional effective components, and has the functions of resisting oxidation, aging, insomnia and depression and soothing the nerves.
Owner:SHANGHAI INST OF TECH

Production method of navel orange compound enzyme tea

The invention provides a production method of navel orange compound enzyme tea. The navel orange compound enzyme tea is prepared as follows: navel oranges, pineapples and apples rich in enzyme components are taken as main materials, white granulated sugar and white vinegar are added, alcohol fermentation is firstly performed with yeast, lactic acid bacteria are added for enzyme fermentation, and treatment is performed. Complete fermentation is realized, so that multiple enzymes, vitamins, amino acids, microelements and enzyme components originally contained in selected composition materials ofthe product are fully released, small molecular substances easy to absorb by the human are obtained, excellent strains and advanced process technologies are selected in production of the product, conversion and synthesis of enzyme can be effectively promoted in the fermentation process, so that the product has significant effects of regulating gastrointestinal functions, relaxing bowels, helpingdigestion, removing toxins, maintaining beauty and the like and is convenient to use and carry. The tea is produced with the traditional fermentation technology and belongs to the technical field ofbioengineering.
Owner:JIANGXI GUOGUO BIOTECH CO LTD

Aged health-care old white tea and processing method thereof

The invention relates to a processing method of aged health-care old white tea. The processing method comprises the steps of (1) performing picking; (2) performing withering; (3) performing piling; (4) performing drying; (5) performing storing; (6) mixing with dried orange peel powder; and (7) performing styling and drying. According to the method, the aged health-care old white tea is prepared bya white tea technology, the making method of the white tea is unique in technology, and the white tea does not need to be fried or kneaded, so that the permeability of cell membranes and enzymatic activity can be reserved to the maximum extent, leaf cells are subjected to a series of slow hydrolyzing and oxidation reaction under the situation that the leaf cells are not damaged or slightly damaged, and conjugated non-volatile precursor substances are converted into free volatile aroma components under the action of hydrolase, and foundation is established for unique color, fragrance and tastewhich are formed in the later stage.
Owner:朱文伟

Novel hyaluronase variants with improved stability and pharmaceutical compositions containing same

The present application discloses novel PH20 variants or fragments thereof, human hyaluronidase having improved thermal stability and enzymatic activity, wherein the hyaluronidase is an enzyme that hydrolyzes hyaluronic acid, in particular to PH20 variants or fragments thereof comprising one or more amino acid residue substitutions in a variant having the amino acid sequence of SEQ ID NO: 3, and one or more amino acid residues are selectively deleted at the N-terminus and / or C-terminus.
Owner:ALTEOGEN

Method for extracting soil mircoorganism carbon-sequestrated enzyme

The invention discloses a method for extracting a soil mircoorganism carbon-sequestrated enzyme, comprising the following steps: A: adding soil to a precooled ion remover and a phosphate buffer in sequence; evenly blending, carrying out centrifugation on the mixture at room temperature and collecting sediment; B: adding the sediment obtained in the step A to a cell sample protein extracting solution, carrying out vortex blending, breaking cells by using an ultrasonic method in ice-bath, carrying out centrifugation to collect supernate, adding solid ammonium sulphate until the solubility is reached, oscillating while blending, carrying out centrifugation to collect precipitated protein, adding a dissolved solution to dissolve the protein, and preserving the dissolved protein; and C: placing 1,5-ribulose diphosphate carboxylase / oxygenase protein solution obtained in the step B in water bath to recover enzymatic activity and using an ultraviolet spectrophotometer to measure the activity of 1,5-ribulose diphosphate carboxylase / oxygenase and the nanomole enzymatic activity of fixed CO2 in each kilogram of the soil. The simple, convenient and fast method has the advantage of good repeatability of measurement results and suitability for extraction and activity measurement of soil mircoorganism carbon-sequestrated key enzyme 1,5-ribulose diphosphate carboxylase / oxygenase.
Owner:INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI

Preparation method of exosome bionic preparation for synergistically promoting wound healing and preparation thereof

The invention discloses a preparation method of an exosome bionic preparation for synergistically promoting wound healing and a preparation thereof. The preparation method of the preparation comprises the following steps: preparing catalase-photosensitizer micelle, preparing ROS response lipidosome carrying catalase-photosensitizer micelle, extracting, separating and purifying exosome, mixing the lipidosome with the exosome, mediating membrane fusion between the liposome and the exosome by an extrusion method, and mixing the membrane fusion carrier and gel, and uniformly stirring the mixture to prepare the preparation. According to the preparation prepared by the invention, photodynamic therapy and biological therapy are combined, catalase-photosensitizer micelles are wrapped by a membrane fusion carrier, accumulated hydrogen peroxide is eliminated on a wound surface, oxygen is supplemented, and the photosensitizer generates controllable singlet oxygen to resist inflammation and sterilize, so that favorable conditions are created for treating the wound surface by the exosome; meanwhile, the gel provides a favorable moist environment for wound healing, and the gel is high in safety, good in stability, convenient to apply, simple in processing technology, low in cost and good in application prospect.
Owner:苏州尼达生物科技有限公司

Mycoplasma arthritis arginine deiminase mutant and its application

The invention discloses an arginine deiminase mutant from an arthritis-type mycoplasma, a gene, a polyethylene glycol modified substance and an application thereof. The arginine deiminase mutant is a dipolymer which is formed by two protein subunits, wherein amino sequences of the protein subunits are represented as SEQ ID No.2 in a sequence table. The arginine deiminase mutant is increased in expression quantity in escherichia coli, is increased in renaturation efficiency, is not changed in enzyme activity and is suitable for industrial production. The polyethylene glycol modified substance of the arginine deiminase mutant can be used for preparing anti-tumor drugs, such as anti-hepatoma drugs, anti-leukemia drugs and anti-melanoma drugs.
Owner:SHANGHAI INST OF PHARMA IND CO LTD +1

A kind of β-galactosidase gala and its application

The invention relates to a β-galactosidase GalA and its application. The amino acid sequence of the β-galactosidase GalA is shown in SEQ ID NO.1. The invention provides a preparation method for recombinant expression and purification of the β-galactosidase GalA, the enzyme activity of the fermentation broth can reach 3795.6 U / mL, the purity of one-step purification is >97%, and the recovery rate is 79.5%. The β-galactosidase GalA of the present invention has good thermal stability and storage stability, and the optimum reaction temperature is 50°C, and it still retains 89.7% of the enzyme activity after continuous incubation at 50°C for 24 h. At room temperature (30 ℃) stored for 30 days, still can retain 81.5% of the enzyme activity. The β-galactosidase GalA of the invention has high activity, good stability, strong ability to degrade lactose, and good application potential.
Owner:QINGDAO UNIV +1

A kind of manganese dioxide-based nano drug carrier and its preparation method and application

The invention relates to a manganese dioxide-based nanometer drug carrier, and innovatively combines starvation therapy and gas therapy for cancer treatment. Specifically, the use of amidation reaction was the first to introduce folic acid targeting molecules on human serum protein, and then the nano-manganese dioxide carrier was cultured in the protein by biomineralization method. Glucose oxidase and L-arginine were simultaneously loaded by physical adsorption. The advantages are: 1. The targeted drug delivery efficiency and dispersibility of the nano-manganese dioxide are greatly improved. 2. Use specific chemical reactions in cancer cells to consume endogenous glucose and release nitric oxide gas to kill cancer cells through starvation and gas therapy. 3. The synthetic carrier material is degradable, and the loaded enzymes and amino acids are endogenous substances in the human body, which have no toxic and side effects on the human body. 4. Significant anti-cancer effect, can kill cancer cells at a very small concentration of biological enzymes.
Owner:QUFU NORMAL UNIV

Preparation method of superparamagnetic carrier for crosslinking and immobilizing lipase

The invention discloses a method for preparing a super paramagnetic carrier of a cross-linking immobilized lipase. The preparation of the super paramagnetic carrier is realized by preparing a nano-magnetic microsphere Fe3O4, coating the nano-magnetic microsphere Fe3O4, functionally modifying the coated nano-magnetic microsphere Fe3O4. A thermo-sensitive material, namely N-isopropylacrylamide, is introduced into the super paramagnetic carrier prepared according to the method, and the structural performance of the carrier can be changed through temperature control, so that the lipase which is immobilized through the super paramagnetic carrier can get out of the interface performance control to realize direct reactions among alcohols, acids and lipids, and separation of the reaction products is facilitated; moreover, lipase activity control through alpha coverage is facilitated. The super paramagnetic carrier is a super paramagnetic polymer microsphere based on the mode of lipase interface activity action; the lipase can be immobilized through the super paramagnetic polymer microsphere; the immobilized lipase is high in magnetic separation performance; 90% or higher of the lipase activity is kept; the detect that the lipase activity in an enzymatic reaction depends on the interface effect is overcome.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Method for producing continuously 4-hydroxycinnamyl alcohol using multi-strain immobilized cell composition

The invention relates to a method for producing continuously 4-hydroxycinnamyl alcohol using a multi-strain immobilized cell composition. Strain A used herein is recombinant strain E. coli M15-4Cl-CCR, and strain B is recombinant strain E. Coli M15-CAD; the method comprises the specific steps: 1, culturing strains; 2, immobilizing the strains to obtain respectively strain A immobilized cell particles and strain B immobilized cell particles; 3, producing 4-hydroxycinnamyl alcohol with the immobilized cells. The 4-hydroxycinnamyl alcohol is produced using modern bioengineering technology, and the application of the method for producing continuously with the multi-strain immobilized cell composition in the synthesis of plant-origin metabolite has important practical significance; high-performance liquid chromatography tandem mass spectrometry detection is established in the meantime, and more effective analytical means is provided for the aromatic polymers.
Owner:BEIJING FORESTRY UNIVERSITY +1

A preparation method and preparation of an exosome biomimetic preparation that synergistically promotes wound healing

ActiveCN113577272BPromote new creationAvoiding the Risks of Whole Body Laser ExposureAntibacterial agentsPeptide/protein ingredientsLiposomeBiology
The invention discloses a method for preparing an exosome biomimetic preparation that synergistically promotes wound healing and its preparation. The preparation of the preparation includes: preparation of catalase-photosensitizer micelles, carrying catalase-photosensitizer Micellar ROS-responsive liposome preparation, exosome extraction, separation and purification, mixing liposomes and exosomes, extrusion mediating membrane fusion between liposomes and exosomes, and membrane fusion The carrier is mixed with the gel and stirred evenly. The preparation prepared by the present invention combines photodynamic therapy and biological therapy, uses membrane fusion carrier to wrap catalase-photosensitizer micelles, eliminates accumulated hydrogen peroxide on the wound surface and supplements oxygen, and the photosensitizer produces a controllable singlet state Oxygen anti-inflammatory and sterilizing creates favorable conditions for exosomes to treat wounds. At the same time, the gel itself also provides a favorable moist environment for wound healing. It has high safety, good stability, convenient application, simple processing technology, and low cost. It has a good application prospect.
Owner:苏州尼达生物科技有限公司

Preparation method of cactus fruit and vegetable enzyme and prepared cactus fruit and vegetable enzyme

The invention relates to the field of the preparation of fruit and vegetable enzyme, and in particular relates to a preparation method of cactus fruit and vegetable enzyme and prepared cactus fruit and vegetable enzyme. The preparation method of the cactus fruit and vegetable enzyme comprises the following steps: selecting cactus as well as fruits and vegetables, chopping or slicing the cactus as well as the fruits and vegetables so as to obtain fermentation materials; mixing a culture with the fermentation materials; adding the fermentation materials to a fermentation container in a mode of one layer of the fermentation materials and one layer of edible sugar, wherein the fermentation materials account for 1 / 2-3 / 4 of the volume of the fermentation container, the top layer is edible sugar and the ratio of the addition of the edible sugar to the weight of the fermentation materials is at (0.5-0.6): 1; after filling the tank, spraying edible white vinegar to the surfaces to the materials in the tank, and then fermenting for 150-200 days in a sealed manner so as to obtain the cactus fruit and vegetable enzyme. The preparation method of the cactus fruit and vegetable enzyme disclosed by the invention can eliminate uncontrollable factors in original natural fermentation through mixed fermentation of one or more probiotics, so that the safety of a finished product is improved. A substance obtained from fermentation is rich in nutritional component, and is especially high in SOD (superoxide dismutase) activity.
Owner:苏剑锋
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