Ultrasonic-assisted extraction method for anthocyanin in hibiscus syriacus petals

A hibiscus petal and auxiliary extraction technology, which is applied in the field of extraction of plant secondary metabolites, can solve the problems of reducing extraction rate, oxidation, and increasing costs, and achieve the effect of improving extraction efficiency

Inactive Publication Date: 2014-02-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, anthocyanin extract is obtained by taking the freeze-dried powder of the residual residue after squeezing blueberry juice, adding acidic methanol solution according to the volume ratio of solid to liquid at 1:5 to 1:20, and ultrasonically extracting at 20 to 50°C for 10 to 40 minutes; Technology Ultrasonic time is too long, energy consumption is too high, increase the cost of the product; and may cause the decomposition or oxidation of anthocyanin, reduce the extraction rate

Method used

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  • Ultrasonic-assisted extraction method for anthocyanin in hibiscus syriacus petals
  • Ultrasonic-assisted extraction method for anthocyanin in hibiscus syriacus petals
  • Ultrasonic-assisted extraction method for anthocyanin in hibiscus syriacus petals

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Experimental program
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Embodiment 1

[0020] This embodiment includes the following steps:

[0021] 1) Collect pink hibiscus petals in full flowering stage, grind them into powder in liquid nitrogen, and weigh 1g of hibiscus petal powder.

[0022] 2) The extractant is 0.1% (v / v) hydrochloric acid methanol, the liquid-to-material ratio is 20:1, the ultrasonic extraction temperature is 40°C, the ultrasonic power is 300W, and the ultrasonic extraction time is in the range of 1-7min.

[0023] 3) The anthocyanin content of hibiscus flower was determined by pH differential method. Draw 1 mL of anthocyanin extract, dilute to 3 mL with pH 1.0 and pH 4.5 buffer respectively. Stand at room temperature for 15 minutes, use distilled water as a reference, measure the absorbance values ​​at the maximum absorption wavelengths of 520nm and 700nm, and calculate the content of anthocyanins. With cyanidin-3-glucoside as the anthocyanin standard, the total anthocyanin content is represented by cyanidin-3-glucoside content.

[0024...

Embodiment 2

[0026] This embodiment includes the following steps:

[0027] 1) Collect pink hibiscus petals in full flowering stage, grind them into powder in liquid nitrogen, and weigh 1g of hibiscus petal powder.

[0028] 2) The extractant is 0.1% (v / v) methanol hydrochloride. The ultrasonic extraction temperature is 40°C, the ultrasonic power is 300W, and the ultrasonic extraction time is 4min. The ratio of g) to extract hibiscus petal anthocyanins under the condition that the liquid-to-solid ratio is 10:1-70:1.

[0029] 3) The anthocyanin content of hibiscus flower was determined by pH differential method. Draw 1 mL of anthocyanin extract, dilute to 3 mL with pH 1.0 and pH 4.5 buffer respectively. Stand at room temperature for 15 minutes, use distilled water as a reference, measure the absorbance values ​​at the maximum absorption wavelengths of 520nm and 700nm, and calculate the content of anthocyanins. With cyanidin-3-glucoside as the anthocyanin standard, the total anthocyanin con...

Embodiment 3

[0032] This embodiment includes the following steps:

[0033] 1) Collect pink hibiscus petals in full flowering stage, grind them into powder in liquid nitrogen, and weigh 1g of hibiscus petal powder.

[0034] 2) The extractant is 0.1% (v / v) hydrochloric acid methanol, the liquid-to-material ratio is 20:1, the ultrasonic power is 300W, the ultrasonic extraction time is 4min, and the ultrasonic extraction temperature is 30-80°C Extract hibiscus petal anthocyanins.

[0035] 3) The anthocyanin content of hibiscus flower was determined by pH differential method. Draw 1 mL of anthocyanin extract, dilute to 3 mL with pH 1.0 and pH 4.5 buffer respectively. Stand at room temperature for 15 minutes, use distilled water as a reference, measure the absorbance values ​​at the maximum absorption wavelengths of 520nm and 700nm, and calculate the content of anthocyanins. With cyanidin-3-glucoside as the anthocyanin standard, the total anthocyanin content is represented by cyanidin-3-gluco...

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Abstract

An ultrasonic-assisted extraction method for anthocyanin in hibiscus syriacus petals belongs to the field of plant secondary metabolite extracting, and comprises: grinding hibiscus syriacus petals in liquid nitrogen into a powder, adding a mixed solution of hydrochloric acid and methanol, and extracting anthocyanin from hibiscus syriacus petals under the conditions of constant-temperature water bath and ultrasonic environment. The method employs the mixed solution of hydrochloric acid and methanol with a ratio of 0.1% (v / v) as the extraction solvent and is assisted by ultrasonic for extracting anthocyanin from hibiscus syriacus petals, and the method helps to substantially reduce the extraction time of hibiscus syriacus anthocyanin and improve the extraction efficiency.

Description

technical field [0001] The invention relates to a plant secondary metabolite extraction technology, in particular to an ultrasonic-assisted hibiscus petal anthocyanin extraction method. Background technique [0002] Hibiscus syriacus L. (Hibiscus syriacus L.) is a deciduous shrub or small tree of Malvaceae Hibiscus. It is a traditional famous flower in my country. According to records, it has It has a cultivation history of nearly 3000 years. Studies have shown that hibiscus petals contain a variety of biologically active substances such as anthocyanins, which have anti-oxidation, free radical scavenging, anti-mutation activity, alleviating liver dysfunction, and anti-tumor functions. Moreover, hibiscus is easy to reproduce and grows fast, so it is a natural plant pigment resource with great development prospects. [0003] Plant metabolites mostly exist inside the cells, and the cells need to be broken during extraction. The conventional extraction methods include decoction...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08C09B61/00
Inventor 刘群录张宝智王婷婷张彦婷李欣唐蓉
Owner SHANGHAI JIAO TONG UNIV
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