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Application of plant secondary metabolite in anaerobic fermentation of biomass

A secondary metabolite and anaerobic fermentation technology, applied in the field of biomass resource utilization, can solve the problems that have not been seen in the field of anaerobic fermentation, and achieve the effects of low cost, accelerated decomposition, and increased methane content

Active Publication Date: 2017-10-27
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant secondary metabolites are rich in types and sources. According to their basic structural characteristics, they are mainly divided into three categories: terpenoids, phenols and nitrogen-containing compounds. They are an important source of natural medicines and industrial raw materials, and are currently widely used in medicines and spices. , cosmetics, fuel and food, but has not yet seen its application in the field of anaerobic fermentation

Method used

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  • Application of plant secondary metabolite in anaerobic fermentation of biomass
  • Application of plant secondary metabolite in anaerobic fermentation of biomass
  • Application of plant secondary metabolite in anaerobic fermentation of biomass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Preparation of compound biogas fermentation bacterial agent

[0026] The compound biogas fermentation bacterial agent is prepared by the following method: (1) Cultivate the biogas fermentation bacterial agent: inoculate Methanococcus mazei, Methanobacterium subterraneanus, Methanosarcina siciliae, and bamboo-shaped methane mane into the culture medium respectively from the slope Cultivate at a temperature of 20-25°C for 20-30 hours, then culture at a low temperature of 2-10°C for 70-96 hours, remove the methane in the upper layer of the culture medium, fill with carbon dioxide, and obtain a biogas-producing microbial fermentation liquid; cold methanogens , Methanosarcina acidophilus, were inoculated into the culture medium from a slant and cultured at a temperature of 20-23°C for 24 hours, and then cultured at 2-5°C for 20-40 hours and then adjusted to a temperature of 20-22°C for 50- After 70 hours, remove the methane in the upper layer of the culture med...

Embodiment

[0027] Example: Method for Paeoniflorin to Promote Anaerobic Methanogenic Performance of Various Agricultural Wastes

[0028] Material pretreatment method: after taking back Miscanthus stalks, air-dry them naturally, break them into small pieces of about 1 cm, and store them in a cool and dry place for later use. The pig manure was artificially dried and cleaned, and placed in a refrigerator at 4°C for later use. The anaerobic activated sludge is taken from the CSTR reactor, and the water content is adjusted after retrieval, stored in a sealed container and fed with nitrogen to isolate it from oxygen in the atmosphere, and keep the temperature in the container at 15-25°C ; Add nutrients to the container under the condition of isolating the air, the daily nutrient addition amount is 0.5-1.5% of the sludge volume in the container, and export the gas in the container at any time.

[0029] Paeoniflorin addition method: put different concentrations of paeoniflorin and materials in...

Embodiment 3

[0034] Embodiment 3: the method that flavonol glycosides promotes the anaerobic methanogenesis performance of various agricultural wastes

[0035] Material pretreatment method: take back Miscanthus stalks and air-dry them naturally, pass through a 20-mesh sieve after crushing, and store them in a cool and dry place for later use. The pig manure was artificially dried and cleaned, and placed in a refrigerator at 4°C for later use. The anaerobic activated sludge is taken from the CSTR reactor, and the water content is adjusted after retrieval, stored in a sealed container and fed with nitrogen to isolate it from oxygen in the atmosphere, and keep the temperature in the container at 15-25°C ; Add nutrients to the container under the condition of isolating the air, the daily nutrient addition amount is 0.5-1.5% of the sludge volume in the container, and export the gas in the container at any time.

[0036] Addition method of flavonol glycosides: The amount of flavonol glycosides ...

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Abstract

The invention belongs to the field of resource utilization of biomass and particularly provides application of a plant secondary metabolite in anaerobic fermentation of biomass. By adding plant secondary metabolite into anaerobic fermentation raw materials of the biomass, microbial activities are promoted, so that the content of methane produced through the anaerobic fermentation of the biomass is increased. The invention is mainly applied to the field of producing methane through the anaerobic fermentation of biomass such as forestry and agricultural waste such as straws, energy crops such as Chinese silvergrass, animal waste, kitchen waste or other solid waste; by adding a small amount of the plant secondary metabolite into an anaerobic fermentation system, the starting of the anaerobic fermentation can be promoted, and the yield of methane can be increased.

Description

technical field [0001] The invention relates to a new application of plant secondary metabolites in promoting anaerobic digestion of biomass and improving methane production efficiency, and belongs to the field of biomass resource utilization. Background technique [0002] Biomass includes all plants, microorganisms, animals that feed on plants and microorganisms and their wastes, mainly referring to agricultural and forestry wastes, energy plants, livestock and poultry wastes, etc. my country's biomass resources are very rich, if they can be efficiently developed and utilized, it will play an important role in solving my country's energy, ecological and environmental problems. Anaerobic fermentation technology is one of the important ways to realize the efficient development and utilization of biomass resources. Anaerobic fermentation of biomass refers to the conversion of biomass raw materials into human beings through specific metabolic pathways under suitable conditions ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02
CPCC12P5/023Y02E50/30
Inventor 田云王翀卢向阳杨辉
Owner HUNAN AGRICULTURAL UNIV
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