42 results about "Peroxidase like" patented technology

The invention discloses a silver composite iron oxide fiber as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving polyvinylpyrrolidone in an organic solvent so as to prepare a polymer solution; dissolving ferric nitrate, silver nitrate and citric acid into water so as to prepare a salt solution; mixing the polymer solution and the salt solution, and uniformly stirring so as to prepare a precursor sol solution with preset viscosity; preparing the precursor sol solution into gel fibers by adopting an electrospinning technique; and drying and calcining the gel fibers, thereby obtaining the silver composite iron oxide fiber after calcining, wherein a mass ratio of the silver to iron to citric acid is 1:(5-8):(45-50); and the calcining process comprises the sub-steps of heating to the temperature of 300+ / -10 DEG C, maintaining the temperature for a period of time, heating to the temperature of 450+ / -10 DEG C, and then maintaining the temperature for a period of time. The silver composite iron oxide fiber prepared by the preparation method has peroxidase-like activity and is high in enzymatic activity and high in stability.

The invention discloses a preparation method of peroxidase-like copper hydroxide nanorods. The preparation method comprises the following steps: dissolving anhydrous copper chloride and cuprous chloride in a molar ratio of 1:(0.5-1.5) in 50-90mL of deionized water, and uniformly mixing the materials by stirring for 10-30min in a water bath with a water temperature of 30-70 DEG C to obtain a lightgreen mixed solution; then taking ammonia water with the mass concentration of 36-38%, dropwise adding the ammonia water with a disposable dropper to the obtained mixed solution, testing the pH valuewhile dropping, adjusting the pH value to 9.5-11.5, carrying out stirring for 30-80min to ensure that the original solution turns dark blue; and centrifuging the product at a speed of 9000-11000r / minfor 5-6 minutes, washing the product with deionized water, and carrying out vacuum drying at 35-45 DEG C to obtain sky blue solid nanorods with lengths of 150nm to 2[mu]m and diameters of 30-50nm. Asthe copper hydroxide nanorods provided by the invention do not contain noble metals and proteins for modification, the cost is very low; and the preparation is simple and rapid. The invention also discloses an application and a method of the peroxidase-like copper hydroxide nanorods.

The invention relates to a preparation method of a MOF compound biological enzyme wound healing agent. The method comprises the following steps: 1) synthesizing MOF; 2) compounding MOF and a biological enzyme; and 3) performing fibrin gel wrapping. MOF can use the peroxidase-like activity function to convert H2O2 into highly toxic .OH for antibacterial application; glucose oxidase can continuouslyconvert non-toxic glucose into abundant gluconic acid and H2O2, the gluconic acid can reduce the pH value from 7 to 3-4, so that the peroxidase-like activity of the MOF can be significantly activated; H2O2 can provide the conversion raw material; and fibrin gel can effectively control the slow release of drugs to achieve the purpose of long-term treatment, and promote wound healing.

The invention relates to a chemical sensor in the field of analytical chemistry and specifically relates to a sensor for L-cysteine detection and a detection method. The sensor comprises suspension liquid of calcined perovskite oxide nano-powder, dye solution and peroxysulfate solution. The dye solution is methylene blue solution or rhodamine 6G solution. The sensor is convenient for preparation and cheap in price. Surfaces of nanoparticles do not need to be modified. The invention also provides the detection method. Through utilization of inducing and shielding effects of the L-cysteine for peroxidase-like activity of perovskite nanoparticles, die decoloring activation performance of the perovskite nanoparticles is reduced. A linear relationship between die absorbance and L-cysteine concentration is established. High sensitivity detection for the L-cysteine is realized. Detection is carried out at normal temperature. A pH value of the solution does not need to be controlled. Detectionis convenient and rapid. Cost is low. Detection sensitivity is high. A detection linear range is wide and is 56nM-56muM.

The invention discloses a cobaltosic oxide micro-ribbon with high peroxidase-like activity and stability and a preparation method therefor. According to the Co3O4 micro-ribbon with high peroxidase-like activity and stability, the micro-ribbon is of a ribbon-shaped structure formed through connecting Co3O4 single-crystal grains. The preparation method comprises the steps: firstly, dissolving polyvinyl pyrrolidone (PVP) into ethanol, adding a cobalt nitrate solution into the solution after the PVP is completely dissolved, and carrying out uniform mixing, so as to obtain spinning sol; then, carrying out spinning on the spinning sol by adopting an electrostatic spinning method, so as to obtain gelatinous fibers, and carrying out drying; and finally, carrying out staged calcination on the dried gelatinous fibers, thereby obtaining the Co3O4 micro-ribbon. When the micro-ribbon is used as mimetic peroxidase, activity can be shown in a relatively wide pH and temperature range; a sample shows relatively good affinity to H2O2 and TMB; and meanwhile, the micro-ribbon with relatively high specific surface area shows relatively high peroxidase-like activity and stability.

An EGFR immunohistochemical detection kit based on Fe<3>O4 nanoprobes and a using method thereof are disclosed. The kit includes a citric acid restoring powder agent, an endogenous peroxidase blocker, a non-specific binding site blocker, a DAB concentrate liquid, a DAB chromogenic substrate, a DAB diluting liquid, the Fe<3>O4 nanoprobes, a TBS powder agent and a hematoxylin dyeing liquor. A novel method of bonding nanometer particles and an antibody is provided to skillfully combine high peroxidase-like activity of the Fe<3>O4 nanometer particles and biological characteristics of an EGFR single-domain antibody, and a high amount of the EGFR single-domain antibody is loaded on the surfaces of the prepared Fe<3>O4 nanoprobes. The Fe<3>O4 nanoprobes are formed by the Fe<3>O4 nanometer particles surfaces of which are loaded with metal ions and the EGFR single-domain antibody the C end of which is provided with His-Tags, and has advantages of a small size, good stability, high specificity, high sensitivity and high catalytic activity. The EGFR expression level of a tumor pathological tissue can be rapidly and conveniently detected through the kit, thus providing bases for tumor treatment.

The invention discloses a ferriporphyrin organic frame material peroxidase mimic. The erriporphyrin organic frame material is prepared from 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic acid dianhydride and 5,10,15,20-tetra(4-aminophenyl) porphyrin iron through a solvothermal technology. The ferriporphyrin organic frame material has excellent peroxidase-like catalysis activity, can rapidly and effectively catalyze 3,3',5,5'-tetramethyl benzidine oxidation for generating a typical blue solution in the presence of hydrogen peroxide, and can be used for detecting low-concentration H2O2, and the absorbance of a catalysis product is linearly related with the concentration, in a concentration range, of the hydrogen peroxide. The linear range of detection H2O2 is 1-200 [mu]M, and the detection limit is 0.14 [mu]M.

A method for improving peroxidase-like activity of nanozyme and a product thereof are disclosed, which relate to the field of artificial enzymes in biochemistry. The method adopts a hydrogen peroxide solution with high concentration to treat the VO2(B) powder for obtaining a product with high peroxidase-like activity. Compared with the pure VO2(B) powder, the peroxidase-like activity of the product obtained by the method is increased by 4 to 12 times. The method provided by the present invention adopts raw materials with low cost and mild reaction conditions, is simple in operation and low in cost, which is conducive to batch preparation. The powder product obtained by the method is able to be applied to detect hydrogen peroxide, glucose, etc., and has great application prospects in biosensing, industrial wastewater treatment and sewage treatment.

The invention discloses a method for improving the adaptive capacity of soybeans in saline-alkali soil. The method includes the steps that nutrient soil and perlite are mixed evenly to serve as bacterial manure matrixes, and then nodule bacteria fluid and the bacterial manure matrixes are mixed and stirred evenly to serve as nodule bacteria manure; soybean seedlings are transplanted into the nodule bacteria manure and are cultivated under the condition with the temperature being 23 DEG C to 27 DEG C, the illumination intensity being 2,000 lx to 2,500 lx and the illumination time being 12 h / d. The method utilizes the nodule bacteria manure to promote the whole plant fresh weight of the soybeans to be increased by from 22.50% to 125% in the soil with high-concentration salt, remarkably improves the salt tolerance related-gene expression level of the soybeans, and improves the mRNA expression level of peroxidases like APX and SOD by three times to eight times.