42 results about "Peroxidase like" patented technology

The invention discloses a cobaltosic oxide micro-ribbon with high peroxidase-like activity and stability and a preparation method therefor. According to the Co3O4 micro-ribbon with high peroxidase-like activity and stability, the micro-ribbon is of a ribbon-shaped structure formed through connecting Co3O4 single-crystal grains. The preparation method comprises the steps: firstly, dissolving polyvinyl pyrrolidone (PVP) into ethanol, adding a cobalt nitrate solution into the solution after the PVP is completely dissolved, and carrying out uniform mixing, so as to obtain spinning sol; then, carrying out spinning on the spinning sol by adopting an electrostatic spinning method, so as to obtain gelatinous fibers, and carrying out drying; and finally, carrying out staged calcination on the dried gelatinous fibers, thereby obtaining the Co3O4 micro-ribbon. When the micro-ribbon is used as mimetic peroxidase, activity can be shown in a relatively wide pH and temperature range; a sample shows relatively good affinity to H2O2 and TMB; and meanwhile, the micro-ribbon with relatively high specific surface area shows relatively high peroxidase-like activity and stability.

An EGFR immunohistochemical detection kit based on Fe<3>O4 nanoprobes and a using method thereof are disclosed. The kit includes a citric acid restoring powder agent, an endogenous peroxidase blocker, a non-specific binding site blocker, a DAB concentrate liquid, a DAB chromogenic substrate, a DAB diluting liquid, the Fe<3>O4 nanoprobes, a TBS powder agent and a hematoxylin dyeing liquor. A novel method of bonding nanometer particles and an antibody is provided to skillfully combine high peroxidase-like activity of the Fe<3>O4 nanometer particles and biological characteristics of an EGFR single-domain antibody, and a high amount of the EGFR single-domain antibody is loaded on the surfaces of the prepared Fe<3>O4 nanoprobes. The Fe<3>O4 nanoprobes are formed by the Fe<3>O4 nanometer particles surfaces of which are loaded with metal ions and the EGFR single-domain antibody the C end of which is provided with His-Tags, and has advantages of a small size, good stability, high specificity, high sensitivity and high catalytic activity. The EGFR expression level of a tumor pathological tissue can be rapidly and conveniently detected through the kit, thus providing bases for tumor treatment.

The invention discloses a ferriporphyrin organic frame material peroxidase mimic. The erriporphyrin organic frame material is prepared from 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic acid dianhydride and 5,10,15,20-tetra(4-aminophenyl) porphyrin iron through a solvothermal technology. The ferriporphyrin organic frame material has excellent peroxidase-like catalysis activity, can rapidly and effectively catalyze 3,3',5,5'-tetramethyl benzidine oxidation for generating a typical blue solution in the presence of hydrogen peroxide, and can be used for detecting low-concentration H2O2, and the absorbance of a catalysis product is linearly related with the concentration, in a concentration range, of the hydrogen peroxide. The linear range of detection H2O2 is 1-200 [mu]M, and the detection limit is 0.14 [mu]M.

A method for improving peroxidase-like activity of nanozyme and a product thereof are disclosed, which relate to the field of artificial enzymes in biochemistry. The method adopts a hydrogen peroxide solution with high concentration to treat the VO2(B) powder for obtaining a product with high peroxidase-like activity. Compared with the pure VO2(B) powder, the peroxidase-like activity of the product obtained by the method is increased by 4 to 12 times. The method provided by the present invention adopts raw materials with low cost and mild reaction conditions, is simple in operation and low in cost, which is conducive to batch preparation. The powder product obtained by the method is able to be applied to detect hydrogen peroxide, glucose, etc., and has great application prospects in biosensing, industrial wastewater treatment and sewage treatment.

The invention discloses a method for improving the adaptive capacity of soybeans in saline-alkali soil. The method includes the steps that nutrient soil and perlite are mixed evenly to serve as bacterial manure matrixes, and then nodule bacteria fluid and the bacterial manure matrixes are mixed and stirred evenly to serve as nodule bacteria manure; soybean seedlings are transplanted into the nodule bacteria manure and are cultivated under the condition with the temperature being 23 DEG C to 27 DEG C, the illumination intensity being 2,000 lx to 2,500 lx and the illumination time being 12 h / d. The method utilizes the nodule bacteria manure to promote the whole plant fresh weight of the soybeans to be increased by from 22.50% to 125% in the soil with high-concentration salt, remarkably improves the salt tolerance related-gene expression level of the soybeans, and improves the mRNA expression level of peroxidases like APX and SOD by three times to eight times.