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Preparation method of copper-gold-platinum composite nanomaterial and application of copper-gold-platinum composite nanomaterial in detection of cysteine

A composite nanomaterial, cysteine ​​technology, applied in the detection of cysteine, in the field of preparation of copper-gold-platinum composite nanomaterials, can solve the complex preparation process of graphene oxide, lack of uniformity and monodispersity Good, high equipment requirements, to achieve the effect of easy promotion, low equipment requirements, and simple preparation methods

Active Publication Date: 2018-11-06
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Graphene oxide has a flake structure, and its size is generally at the micron level. As a catalyst, its uniformity and monodispersity are not good enough
In addition, the preparation process of graphene oxide is relatively complicated and requires high equipment

Method used

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  • Preparation method of copper-gold-platinum composite nanomaterial and application of copper-gold-platinum composite nanomaterial in detection of cysteine
  • Preparation method of copper-gold-platinum composite nanomaterial and application of copper-gold-platinum composite nanomaterial in detection of cysteine
  • Preparation method of copper-gold-platinum composite nanomaterial and application of copper-gold-platinum composite nanomaterial in detection of cysteine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing a copper-gold-platinum composite nanomaterial, comprising the following steps:

[0023] Step 1, Preparation of Cu@Au

[0024] Add CuSO to 5 ml of water 4 (50 μl, 0.1 M) and sodium citrate (50 μl, 0.1 M) in water, then add 1 ml of fresh sodium borohydride solution (3.8 mg / 4 ml H 2 O), after 15 min, add HAuCl 4 Aqueous solution (50μl, 0.1M), after stirring for 15-25 minutes, store at 12-16°C for 24 hours to obtain copper / gold composite nanomaterial Cu@AuNPs, put it in the refrigerator and keep it in the dark for later use;

[0025] Step 2, Preparation of Cu@Au / Pt

[0026] Take 2ml of Cu@AuNPs prepared above and add to 5ml of 1.0 mM K 2 PtCl 6 In the solution, after heating to 80°C while stirring, 5ml of L-ascorbic acid (ascorbic acid) with a concentration of 5mM was added dropwise, and the stirring was continued for 30min to obtain the copper-gold-platinum composite nanomaterial.

Embodiment 2

[0027] Example 2 Effect of pH value on the peroxidase-like activity of copper-gold-platinum composite nanomaterials

[0028] The pH range is 3.6-5.6, and it can be seen from the above experiments that when the pH is 3.8, the peroxidase-like activity is the strongest.

Embodiment 3

[0029] Example 3H 2 o 2 The effect of concentration on the peroxidase-like activity of copper-gold-platinum composite nanomaterials

[0030] h 2 o 2 The concentrations are 0, 1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 8 mM, 10 mM, 15 mM, 20 mM, 30 mM, 40 mM.

[0031] From the above experiments, it can be seen that the H 2 o 2 At a concentration of 20mM, the peroxidase-like activity is the strongest.

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Abstract

The invention discloses a preparation method of a copper-gold-platinum composite nanomaterial and application of the copper-gold-platinum composite nanomaterial in detection of cysteine. The preparation method comprises the following steps of adding a copper sulfate solution and a sodium citrate solution in water, adding fresh NaBH4, adding a HAuCl4 water solution after the solution is pale yellow, stirring, then storing at the temperature of 12-16 DEG C to obtain Cu@AuNPs, and placing in a refrigerator to be refrigerated in a dark place for later use; and adding Cu@AuNPs in a K2PtC16 solution, stirring and heating to 80 DEG C, then dropwise adding ascorbic acid, and continuously stirring until the solution is black. The prepared copper-gold-platinum composite nanomaterial has peroxidase sample activity, can enable TMB and a hydrogen peroxide system to be blue, however the addition of the cysteine can inhibit peroxidase sample activity, and the concentration of the cysteine can be detected visually according to the color change of the system.

Description

technical field [0001] The invention relates to the technical field of chemical testing and analysis, in particular to a preparation method of a copper-gold-platinum composite nanomaterial and its application in detecting cysteine. Background technique [0002] Cysteine ​​plays a very important role in human physiological and pathological processes, and abnormal cysteine ​​content may cause various diseases. Therefore, the quantitative detection of cysteine ​​is of great significance. However, the methods currently used for cysteine ​​detection mainly include enzyme cycle method, electrochemical method, fluorescence method, etc. The sample preparation of these methods is cumbersome and the detection process is complicated. [0003] Application number 2015101805572, named as a method for measuring cysteine ​​based on graphene oxide-nano-platinum composite material, which is characterized in that the graphene oxide-nano-platinum composite material is used to simulate the ac...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00G01N21/78B82Y30/00B82Y40/00
CPCG01N21/78B82Y30/00B82Y40/00B22F9/24B22F1/054
Inventor 姜翠凤包帅魏晓秀杨子润莫贵和
Owner YANCHENG INST OF TECH
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