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A preparation method of copper-gold-platinum composite nanomaterial and its application in the detection of cysteine

A technology of composite nanomaterials and copper-gold, which is applied in the direction of chemical reaction analysis, nanotechnology, nanotechnology, etc., can solve the problems of insufficient uniformity and monodispersity, complex preparation process of graphene oxide, and equipment requirements. High-level problems, to achieve the effect of easy promotion, fast speed, and low equipment requirements

Active Publication Date: 2021-08-13
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Graphene oxide has a flake structure, and its size is generally at the micron level. As a catalyst, its uniformity and monodispersity are not good enough
In addition, the preparation process of graphene oxide is relatively complicated and requires high equipment

Method used

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  • A preparation method of copper-gold-platinum composite nanomaterial and its application in the detection of cysteine
  • A preparation method of copper-gold-platinum composite nanomaterial and its application in the detection of cysteine
  • A preparation method of copper-gold-platinum composite nanomaterial and its application in the detection of cysteine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing a copper-gold-platinum composite nanomaterial, comprising the following steps:

[0023] Step 1, Preparation of Cu@Au

[0024] Add CuSO to 5 ml of water 4 (50 μl, 0.1 M) and sodium citrate (50 μl, 0.1 M) in water, then add 1 ml of fresh sodium borohydride solution (3.8 mg / 4 ml H 2 O), after 15 min, add HAuCl 4 Aqueous solution (50μl, 0.1M), after stirring for 15-25 minutes, store at 12-16°C for 24 hours to obtain copper / gold composite nanomaterial Cu@AuNPs, put it in the refrigerator and keep it in the dark for later use;

[0025] Step 2, Preparation of Cu@Au / Pt

[0026] Take 2ml of Cu@AuNPs prepared above and add to 5ml of 1.0 mM K 2 PtCl 6 In the solution, after heating to 80°C while stirring, 5ml of L-ascorbic acid (ascorbic acid) with a concentration of 5mM was added dropwise, and the stirring was continued for 30min to obtain the copper-gold-platinum composite nanomaterial.

Embodiment 2

[0027] Example 2 Effect of pH value on the peroxidase-like activity of copper-gold-platinum composite nanomaterials

[0028] The pH range is 3.6-5.6, and it can be seen from the above experiments that when the pH is 3.8, the peroxidase-like activity is the strongest.

Embodiment 3

[0029] Example 3H 2 o 2 The effect of concentration on the peroxidase-like activity of copper-gold-platinum composite nanomaterials

[0030] h 2 o 2 The concentrations are 0, 1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 8 mM, 10 mM, 15 mM, 20 mM, 30 mM, 40 mM.

[0031] From the above experiments, it can be seen that the H 2 o 2 At a concentration of 20mM, the peroxidase-like activity is the strongest.

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Abstract

The invention discloses a preparation method of a copper-gold-platinum composite nanometer material and its application in detecting cysteine. Add copper sulfate solution and sodium citrate solution to water, then add fresh NaBH 4 , after the solution turns light yellow, add HAuCl 4 After stirring the aqueous solution, store it at 12-16°C to obtain Cu@AuNPs, put them in the refrigerator and keep them in the dark for later use; take Cu@AuNPs and add them to K 2 PtCl 6 In the solution, heat to 80°C while stirring, then add ascorbic acid drop by drop and continue stirring until the solution turns black. The copper-gold-platinum composite nanomaterial prepared by the present invention has peroxidase-like activity, which can make the TMB and hydrogen peroxide system show blue color, and the addition of cysteine ​​can inhibit the peroxidase-like activity, and the color change according to the system , the visual detection of cysteine ​​concentration can be realized.

Description

technical field [0001] The invention relates to the technical field of chemical testing and analysis, in particular to a preparation method of a copper-gold-platinum composite nanomaterial and its application in detecting cysteine. Background technique [0002] Cysteine ​​plays a very important role in human physiological and pathological processes, and abnormal cysteine ​​content may cause various diseases. Therefore, the quantitative detection of cysteine ​​is of great significance. However, the methods currently used for cysteine ​​detection mainly include enzyme cycle method, electrochemical method, fluorescence method, etc. The sample preparation of these methods is cumbersome and the detection process is complicated. [0003] Application number 2015101805572, named as a method for measuring cysteine ​​based on graphene oxide-nano-platinum composite material, which is characterized in that the graphene oxide-nano-platinum composite material is used to simulate the acti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B22F1/00G01N21/78B82Y30/00B82Y40/00
CPCG01N21/78B82Y30/00B82Y40/00B22F9/24B22F1/054
Inventor 姜翠凤包帅魏晓秀杨子润莫贵和
Owner YANCHENG INST OF TECH
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