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Glucose detection method based on peroxidase-like catalytic properties of graphene quantum dots

A technology of graphene quantum dots and peroxidase, which is applied in the field of optical sensing and achieves good application prospects

Inactive Publication Date: 2017-06-30
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current studies on GQDs are all designed based on the carboxyl groups or charges on their surface, and there are still few studies on the catalytic activity of GQDs.

Method used

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  • Glucose detection method based on peroxidase-like catalytic properties of graphene quantum dots
  • Glucose detection method based on peroxidase-like catalytic properties of graphene quantum dots
  • Glucose detection method based on peroxidase-like catalytic properties of graphene quantum dots

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited thereto.

[0019] Preparation of graphene quantum dots:

[0020] (1) Preparation of graphene oxide: 0.5 g of graphite powder and 0.5 g of sodium nitrate were added to 23 mL of concentrated sulfuric acid with a concentration of 98% by mass, stirred evenly in an ice bath, and then slowly added 3 g of high Potassium manganate, stirred in a 35 °C water bath until the solution forms a brown paste, add 40 mL of ultrapure water and continue to stir for 30 minutes, then add 100 mL of ultrapure water, drop by drop % hydrogen peroxide until the color of the solution turns bright yellow; the product is filtered while hot and cleaned with ultrapure water until the filtrate is neutral, and dried at 50 °C in a vacuum oven to obtain graphene oxide;

[0021] (2) Preparation of graphene sheets: Graphene oxide was placed i...

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Abstract

The invention discloses a glucose detection method based on peroxidase-like catalysis characteristics of graphene quantum dots, and belongs to the technical field of optical sensing. The graphene quantum dots have the peroxidase-like catalytic activity, can catalyze hydrogen peroxide reduction in the presence of hydrogen peroxide, also can oxidize colorless reduced-form tetramethyl benzidine into oxidized-form tetramethyl benzidine to show blue and produce an ultraviolet absorption peak at 652nm. In the presence of oxygen, glucose oxidase catalyzes oxidation of glucose to produce the hydrogen peroxide, the hydrogen peroxide reduction is catalyzed by use of the peroxidase-like catalysis characteristics of the graphene quantum dots, and the reduced-form tetramethyl benzidine in the solution is oxidized to show color. The glucose concentration is greater, the blue color of the solution is deeper, and the ultraviolet absorption strength is stronger, so that fast, sensitive and visual glucose detection can be realized.

Description

technical field [0001] The invention relates to a glucose detection method based on the peroxidase-like catalytic properties of graphene quantum dots, belonging to the technical field of optical sensing. Background technique [0002] Natural enzymes are effective biocatalysts that exist in living organisms. Due to their high biological specificity and catalytic activity, they are used in the fields of medicine, biotechnology, chemical industry and environmental science. Among these natural enzymes, peroxidase is widely used in analytical diagnostics and other fields because it can catalyze various chemical reactions of hydrogen peroxide. Horseradish peroxidase is the most common peroxidase, which is often used in the detection of hydrogen peroxide and glucose. However, the catalytic activity of natural enzymes is easily affected by changes in the natural environment such as pH and temperature. In addition, enzyme extraction, purification and storage costs are relatively h...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 邱建丁张慧张立梁汝萍
Owner NANCHANG UNIV
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