Kit for rapidly detecting resistance mosquitoes through carboxylesterase development method

A kit and mosquito technology, applied in the direction of color/spectral characteristic measurement, analysis through chemical reaction of materials, material analysis through observation of the influence of chemical indicators, etc., can solve problems such as troubles in daily life, and achieve fast Sensitivity, solve the effect of mosquito resistance enhancement, simple operation

Inactive Publication Date: 2012-06-06
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this method is that the accurate value of carboxylesterase activity can be obtained, but the disadvantage is that it must be completed with the aid of a microplate reader, which is troublesome in daily life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the discovery of color development reference card

[0036] The multigenerational resistance screening will be carried out on the Culex mosquito population collected in the field. The method of resistance screening (larva dipping method, which is an international general method) is as follows: put the larvae at the end of the third instar in 100 ml of water, add A series of gradients of parathion, observe the mortality after 24 hours, calculate the LC50, and compare the LC50 of the resistant mosquito with the sensitive mosquito S-LAB strain to obtain the resistance multiple.

[0037] Use the mosquitoes with three resistance levels obtained through screening to perform the following operations:

[0038] 1. Put the adult worms into 1.5ml centrifuge tubes, 15 in each tube, and repeat in 2 tubes.

[0039] 2. Add 800 μl of pre-cooled 50 mM phosphate buffer (pH 7.0) to each of the centrifuge tubes containing the mosquitoes, grind the mosquitoes evenly, and place...

Embodiment 2

[0045] Embodiment 2, the preparation of kit

[0046] The kit consists of buffer solution, tube A (1.5ml centrifuge tube containing 5mg β-naphthyl acetate), tube B (1.5ml centrifuge tube containing 5mg solid blue B salt), a grid (1×1cm) It consists of filter paper and color reference card.

[0047] Phosphate buffer: 50mM, pH7.0; composed of water, sodium dihydrogen phosphate and disodium hydrogen phosphate.

[0048] The color reference card has the following three colorimetric areas: I (sensitive area) is lavender, and the specific color is (C) = 150 (R) + 142 (G) + 160 (B), II (low and medium resistance area) ) is medium purple, the specific color is (C)=122(R)+124(G)+158(B), III (high resistance area) is dark purple, the specific color is (C)=93(R)+78 (G)+117(B).

[0049] If the color displayed by the sample to be tested is close to I by using the kit, the sample to be tested is a sensitive strain. If the color shown by the test kit is close to II, the sample to be tested...

Embodiment 3

[0051] Embodiment 3, the application of kit

[0052] 1. Application of kit

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PUM

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Abstract

The invention discloses a kit for rapidly detecting resistance mosquitoes through a carboxylesterase development method. The kit of the invention, which comprises beta- naphthyl acetate, a fast blue B salt, a phosphoric acid buffer and filter paper, also comprises a development reference card with three colorimetric zones, wherein the color of a sensitizing zone is characterized in that (C)=145-155)(R)+(137-147)(G)+(155-165)(B); the color of a low and middle resistance zone is characterized in that (C)=(117-127)(R)+(119-129)(G)+(153-163)(B); and the color of a high resistance zone is characterized in that (C)=(88-98)(R)+(73-83)(G)+(112-122)(B). The kit has the advantages of simple operation, realization of reaction and development on the filter paper, rapidness and high sensitivity; and the resistance level of the mosquitoes to an organophosphorus pesticide can be obtained by comparing the filter paper with the development reference card, so the type and the concentration of a best pesticide can be obtained, and momentary onsite detection can be realized. The kit which solves a problem of the mosquito resistance enhancement caused by the application of a large amount of the pesticide allows the pollution of the pesticide to environment and the influence of the pesticide to the ecological balance of nature to be effective reduced.

Description

technical field [0001] The invention relates to a kit for rapidly detecting resistant mosquitoes by using a carboxylesterase chromogenic method. Background technique [0002] Due to its special behavior and physiological characteristics, mosquitoes have become the vectors of various diseases, seriously affecting the tourism and catering industries, restricting the development of sanitary cities, and seriously endangering human health. There are many kinds of mosquitoes, and there are many diseases transmitted by biting. Culex mosquito is the main vector of filariasis, Japanese encephalitis and West Nile virus. Anopheles is the main vector of filariasis and malaria. Aedes mosquito It is the main vector of dengue fever and Japanese encephalitis (Hemingway J and Ranson H. Insecticide resistance in insect vectors of humandisease [J]. Annu. Rev. Entomol. 2000, 45: 371-391.). [0003] At present, physical control, chemical control and biological control are mainly adopted (Xu Che...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/25
Inventor 崔峰江红刘洋洋刘斯璐乔传令
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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