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Method for detecting mercury ions by chitosan-gold nanoparticles

A gold nanoparticle and chitosan technology, applied in the field of analysis, can solve the problems of complex sample pretreatment, expensive equipment, time-consuming, etc., and achieve the effects of rapid detection, short time-consuming, and easy operation

Active Publication Date: 2018-05-15
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, existing methods have drawbacks such as time-consuming, laborious, expensive equipment and complicated sample pretreatment

Method used

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  • Method for detecting mercury ions by chitosan-gold nanoparticles
  • Method for detecting mercury ions by chitosan-gold nanoparticles
  • Method for detecting mercury ions by chitosan-gold nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The chitosan-gold nanoparticles provided by the present embodiment adopt the following steps to make:

[0057] 1mL 3mmol / L of HAuCl 4 and 2 mL of 0.003 g / mL chitosan solution were added to 10 mL of deionized water. Then, the mixture was heated to boiling and continued to reflux and stir for 30 min. After the reaction, cool to room temperature.

[0058] Hg 2+ The detection of: first, prepare 14 different concentrations of Hg 2+ solution. Hg per serving 2+ The volume of the solutions is 100uL, and the concentrations are 0, 0.396, 0.792, 1.386, 1.98, 6.93, 1.2, 11.88, 33.66, 49.5, 67.32, 84.15, 100.98 and 134.64umol / L.

[0059] to each Hg 2+ 160 uL of chitosan-gold nanoparticles prepared above were respectively added into the solution, and 14 parts of the first mixed solution were obtained after mixing.

[0060] After the above 14 parts of the first mixed solution were placed at 25°C for 5 minutes, 330 uL of acetic acid-sodium acetate buffer solution with a concent...

Embodiment 2

[0062] The chitosan-gold nanoparticles provided by the present embodiment adopt the following steps to make:

[0063] 1mL 2mmol / L of HAuCl 4 and 2 mL of 0.002 g / mL chitosan solution were added to 10 mL of deionized water. Then, the mixture was heated to boiling and continued to reflux and stir for 20 min. After the reaction, cool to room temperature.

[0064] Hg 2+ The detection of: first, prepare 14 different concentrations of Hg 2+ solution. Hg per serving 2+ The volume of the solutions is 100uL, and the concentrations are 0, 0.396, 0.792, 1.386, 1.98, 6.93, 1.2, 11.88, 33.66, 49.5, 67.32, 84.15, 100.98 and 134.64umol / L.

[0065] to each Hg 2+ 160 uL of chitosan-gold nanoparticles prepared above were respectively added into the solution, and 14 parts of the first mixed solution were obtained after mixing.

[0066] After the above 14 parts of the first mixed solution were placed at 25°C for 5 minutes, 330 uL of acetic acid-sodium acetate buffer solution with a concent...

Embodiment 3

[0068] The chitosan-gold nanoparticles provided by the present embodiment adopt the following steps to make:

[0069] 1mL 4mmol / L of HAuCl 4 and 2 mL of 0.004 g / mL chitosan solution were added to 10 mL of deionized water. Then, the mixture was heated to boiling and continued to reflux and stir for 40 min. After the reaction, cool to room temperature.

[0070] Hg 2+ The detection of: first, prepare 14 different concentrations of Hg 2+ solution. Hg per serving 2+ The volume of the solutions is 100uL, and the concentrations are 0, 0.396, 0.792, 1.386, 1.98, 6.93, 1.2, 11.88, 33.66, 49.5, 67.32, 84.15, 100.98 and 134.64umol / L.

[0071] to each Hg 2+ 160 uL of chitosan-gold nanoparticles prepared above were respectively added into the solution, and 14 parts of the first mixed solution were obtained after mixing.

[0072] After the above 14 parts of the first mixed solution were placed at 25°C for 5 minutes, 330 uL of acetic acid-sodium acetate buffer solution with a concent...

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Abstract

The invention relates to the field of analysis, in particular to a method for detecting mercury ions by chitosan-gold nanoparticles. The method includes: mixing chitosan-gold nanoparticles, Hg<2+> solution, 3,3',5,5'-tetramethyl benzidine solution and H2O2 solution, culturing for 5-30min at 10-90 DEG C, and using an ultraviolet spectrophotometer for detecting mercury ions, wherein the chitosan-gold nanoparticles is prepared by steps: mixing HAuCl4 solution, chitosan solution and deionized water, heating to boil, performing reflux stirring to the end of reaction, and cooling to the room temperature. The chitosan-gold nanoparticles have peroxidase activity which can be enhanced by mercury ions, so that the chitosan-gold nanoparticles can be used for mercury ion detection. The method is simple, convenient, quick and low in time consumption in a whole operating process and beneficial to quick detection of the mercury ions.

Description

technical field [0001] The invention relates to the field of analysis, in particular to a method for detecting mercury ions by using chitosan-gold nanoparticles. Background technique [0002] Mercury is a highly toxic heavy metal that can enter the human body through the food chain or the environment. Even a trace amount of mercury can cause extremely serious harm to the human body. Therefore, the monitoring and trace detection of mercury are of great significance to the environment and food safety, as well as human health. Currently commonly used mercury detection methods include ICP-AES, X-ray fluorescence spectrometry, atomic absorption spectrometry, etc. However, existing methods have drawbacks such as time-consuming, laborious, expensive equipment and complicated sample pretreatment. Therefore, it is imminent to develop a simple and rapid method for detecting mercury ions. Contents of the invention [0003] The object of the present invention is to provide a metho...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 姜翠凤李卓健庞绍平包帅魏晓秀王琪
Owner YANCHENG INST OF TECH
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