PEG-modified magnetic/quaternized chitosan nanoparticles with peroxidase activity, and preparation method and application thereof

Abstract

The invention belongs to the field of composite material, and particularly relates to PEG-modified magnetic / quaternized chitosan magnetic nanoparticles with peroxidase activity, and a preparation method and application thereof. The PEG-modified magnetic / quaternized chitosan magnetic nanoparticles with peroxidase activity comprise highly magnetic Fe3O4 as a magnetic core, a crosslinked polyamide / chitosan polyelectrolyte complex in the middle layer, and a quaternized chitosan and hydrophilic polyethylene glycol chain as the outer shell. The intermediate polymer layer adequately protects the high magnetic property and peroxidase activity of the Fe3O4, and the quaternized chitosan and hydrophilic polyethylene glycol chain as the shell provides long-term water stability and dispersibility for the nanoparticles. Nanoparticles have a fast and efficient "encirclement and suppression" effect on bacterial pathogens. The strategy uses a bacterial capture nanocomposite containing the magnetic nanoparticles with peroxidase-like activity under a trace amount of H2O2.

Description

technical field [0001] The invention belongs to the field of composite materials, and in particular relates to a magnetic / quaternized chitosan magnetic nanoparticle modified with peroxidase activity and a preparation method and application thereof. Background technique [0002] Bacterial infectious diseases can significantly affect human health and result in significant morbidity, mortality, and high healthcare costs. Traditional antibiotic therapy, which has saved millions of lives for decades, remains the treatment of choice for bacterial infections. However, in addition to the disadvantages of high cost and potential health and ecosystem risks, the widespread overuse of antibiotics can lead to multidrug-resistant strains, leading to bacterial infectious diseases that are no longer curable with existing drugs. Currently, thanks to the development of nanotechnology, various nanomaterials play an important role in the fight against bacterial infections, such as metal nanost...

AI Technical Summary

Problems solved by technology

However, in practical applications, such as in biological sciences and medical sciences, the further application of reported magnetic nanoparticles is still limited by the following: (1) Many magnetic nanoparticles lack good biocompatibility, especially those compounded with Au The application of metal nanoparticles such as Ag and Ag in the fields of bioscience and medicine may lead to the leakage and accumulation of heavy metals in the environment (soil, water, human body, etc.); (2) it is difficult to eradicate bacteria with ultra-low concentrations of bacteria; (3) use biomolecular functions The optimized magnetic nanomaterials showed specific bacterial capture and a low interaction rate with all bacterial pathogens when polymicrobial infections were involved; (4) The removal efficiency of bacteria using magnetic nanomaterials was still (5) Due to the van der Waals force, these purely magnetic nanomaterials are usually easy to agglomerate and lack water stability

In addition, although these functional magnetic nanocomposites have good antibacterial activity, the nanoenzyme activity of magnetic particles, this function has been neglected in these magnetic nanocomposite antibacterial agents based on magnetic separation technology.

Images