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Preparation method and application of peroxidase-like copper hydroxide nanorods

A technology of peroxidase and copper hydroxide, which is applied in the field of simulated enzymes and analytical chemistry, can solve the problems of unsatisfactory catalytic activity, high price, time-consuming and labor-consuming, etc., and achieve excellent catalytic activity, low cost, and simple preparation process fast effect

Active Publication Date: 2018-08-28
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] 1) The catalytic activity of these class peroxidases is still unsatisfactory;
[0010] 2) Most of the raw materials used are precious metals, which are expensive and costly; or the raw materials are relatively expensive proteins, and the storage conditions are relatively harsh;
[0011] 3) Operations such as dialysis are used in the preparation process, the preparation process is complicated, the preparation cost is still high, and time-consuming and labor-intensive

Method used

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  • Preparation method and application of peroxidase-like copper hydroxide nanorods
  • Preparation method and application of peroxidase-like copper hydroxide nanorods
  • Preparation method and application of peroxidase-like copper hydroxide nanorods

Examples

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Embodiment 1

[0045] A method for preparing a peroxidase-like copper hydroxide nanorod of the present invention, comprising the steps of:

[0046] 1) Dissolve anhydrous cupric chloride and cuprous chloride with a molar ratio of 1:0.5 in 50 mL of deionized water, stir in a water bath at a water temperature of 30°C for 10 min and mix evenly to obtain a light green mixed solution;

[0047] 2) Add ammonia water with a mass concentration of 36% drop by drop into the mixed solution obtained in the first step using a disposable dropper, test the pH while adding dropwise, adjust the pH to 9.5, and continue stirring for 30 minutes, the color of the original solution turns dark blue;

[0048] 3) Centrifuge at 9000r / min for 5 minutes to separate the product, wash the product with deionized water, and dry it in vacuum at 35°C to obtain a sky blue solid.

[0049] The obtained product was in the form of nanorods ( figure 1 and figure 2 ) has a length of 150nm to 2μm and a diameter of 30 to 50nm, and ...

Embodiment 2

[0055] A method for preparing a peroxidase-like copper hydroxide nanorod of the present invention, comprising the steps of:

[0056] 1) Dissolve anhydrous cupric chloride and cuprous chloride with a molar ratio of 1:1 in 60mL of deionized water, stir in a water bath at a water temperature of 40°C for 15min and mix evenly to obtain a light green mixed solution;

[0057] 2) Add ammonia water with a mass concentration of 37% to the mixed solution obtained in the first step drop by drop using a disposable dropper, test the pH while adding, adjust the pH to 10.5, and continue stirring for 40 minutes, the color of the original solution turns dark blue;

[0058] 3) Centrifuge at a speed of 10000r / min for 5 minutes to separate the product, wash the product with deionized water, and dry it in vacuum at 40°C to obtain a sky blue solid nanorod with a length of 150nm-2μm and a diameter of 30-50nm.

[0059] The application experiment of copper hydroxide peroxidase catalyzing hydrogen perox...

Embodiment 3

[0064] A method for preparing a peroxidase-like copper hydroxide nanorod of the present invention, comprising the steps of:

[0065] 1) Dissolve anhydrous cupric chloride and cuprous chloride with a molar ratio of 1:1 in 60 mL of deionized water, stir in a water bath at a water temperature of 40°C for 15 minutes and mix evenly to obtain a light green mixed solution;

[0066] 2) Add ammonia water with a mass concentration of 37% to the mixed solution obtained in the first step drop by drop using a disposable dropper, test the pH while adding, adjust the pH to 11, and continue stirring for 60 minutes, the color of the original solution turns dark blue;

[0067] 3) Centrifuge at a speed of 10000r / min for 5 minutes to separate the product, wash the product with deionized water, and dry it in vacuum at 40°C to obtain a sky blue solid nanorod with a length of 150nm-2μm and a diameter of 30-50nm.

[0068] The application experiment of copper hydroxide peroxidase catalyzing hydrogen p...

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Abstract

The invention discloses a preparation method of peroxidase-like copper hydroxide nanorods. The preparation method comprises the following steps: dissolving anhydrous copper chloride and cuprous chloride in a molar ratio of 1:(0.5-1.5) in 50-90mL of deionized water, and uniformly mixing the materials by stirring for 10-30min in a water bath with a water temperature of 30-70 DEG C to obtain a lightgreen mixed solution; then taking ammonia water with the mass concentration of 36-38%, dropwise adding the ammonia water with a disposable dropper to the obtained mixed solution, testing the pH valuewhile dropping, adjusting the pH value to 9.5-11.5, carrying out stirring for 30-80min to ensure that the original solution turns dark blue; and centrifuging the product at a speed of 9000-11000r / minfor 5-6 minutes, washing the product with deionized water, and carrying out vacuum drying at 35-45 DEG C to obtain sky blue solid nanorods with lengths of 150nm to 2[mu]m and diameters of 30-50nm. Asthe copper hydroxide nanorods provided by the invention do not contain noble metals and proteins for modification, the cost is very low; and the preparation is simple and rapid. The invention also discloses an application and a method of the peroxidase-like copper hydroxide nanorods.

Description

technical field [0001] The invention belongs to the technical field of imitating enzymes and analytical chemistry, and relates to the preparation of peroxidase imitating enzymes, in particular to a preparation method and application of peroxidase-like copper hydroxide nanorods. Background technique [0002] Under mild conditions, natural enzymes can exhibit high activity and selectivity. Therefore, they are often used as catalysts in the fields of medicine, pharmacy, analysis and detection. However, natural enzymes have several significant disadvantages, including sensitivity of catalytic activity to environmental changes, low stability, etc. Furthermore, their extraction and purification processes are costly and time-consuming. Therefore, researchers are trying to develop potential enzyme mimics. [0003] Since Yan Xiyun et al. (Lizeng Gao, et al., Nature Nanotechnology, 2007, 2:577-583) found that ferroferric oxide magnetic nanoparticles have peroxidase activity, mimeti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G3/02
Inventor 彭银仙宋洪伟刘煜夏大厦
Owner JIANGSU UNIV OF SCI & TECH
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