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A catalyst use of a hydrogen ion containing micromolecule as a peroxidase-like enzyme

A peroxidase and small molecule technology, applied in the field of simulated enzymes, can solve the problems of easy inactivation and denaturation, acid, alkali, thermal instability, and limited wide application, and achieves low price, easy commercial development, and wide application prospects. Effect

Inactive Publication Date: 2018-11-06
GUANGDONG IND TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymes are composed of proteins, which are generally unstable to acid, alkali and heat, and are easily inactivated and denatured, which limits their wide application

Method used

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  • A catalyst use of a hydrogen ion containing micromolecule as a peroxidase-like enzyme
  • A catalyst use of a hydrogen ion containing micromolecule as a peroxidase-like enzyme
  • A catalyst use of a hydrogen ion containing micromolecule as a peroxidase-like enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The mensuration of embodiment 1 peroxidase activity

[0033] Dilute concentrated phosphoric acid to 1mol / L, 0.1mol / L, 0.01mol / L, 0.001mol / L, 0.0001mol / L, 0.00001mol / L concentration of phosphoric acid solution, take 0.1mL solution A (containing 10mmol / L H 2 o 2 , 10mmol / L 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS)) was added to 0.9mL phosphoric acid solutions of different concentrations, and reacted for 1 minute.

[0034] The result is as figure 1 As shown, the small molecule has a good catalytic effect at an appropriate concentration. The darker the color, the better the catalytic effect.

Embodiment 2

[0035] The mensuration of embodiment 2 peroxidase activity

[0036] Dilute concentrated hydrochloric acid to 1mol / L, 0.1mol / L, 0.01mol / L, 0.001mol / L, 0.0001mol / L, 0.00001mol / L hydrochloric acid solution respectively, take 0.1mL solution A (containing 10mmol / L H 2 o 2 , 10mmol / L 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS)) was added to 0.9mL hydrochloric acid solution of different concentrations, and reacted for 1 minute.

[0037] The result is as figure 2 As shown, the small molecule has a good catalytic effect at an appropriate concentration. The darker the color, the better the catalytic effect.

Embodiment 3

[0038] The mensuration of embodiment 3 peroxidase activity

[0039] Dilute concentrated nitric acid to 1mol / L, 0.1mol / L, 0.01mol / L, 0.001mol / L, 0.0001mol / L, 0.00001mol / L concentration of nitric acid solution, take 0.1mL solution A (containing 10mmol / L H 2 o 2 , 10mmol / L 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS)) was added to 0.9mL nitric acid solutions of different concentrations, and reacted for 1 minute.

[0040] The result is as image 3 As shown, the small molecule has a good catalytic effect at an appropriate concentration. The darker the color, the better the catalytic effect.

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Abstract

A catalyst use of a hydrogen ion containing micromolecule as a peroxidase-like enzyme is disclosed. That the hydrogen ion containing micromolecule has peroxidase-like activity is disclosed for the first time. The hydrogen ion containing micromolecule is definite in molecular structure, easily available, high in stability, cheap and prone to commercial development. Based on the use, a novel methodfor detection hydrogen peroxide is successfully established and is successfully applied in test paper for hydrogen peroxide detection. The micromolecule having peroxidase-like activity can also be applied in food, environment, sanitation, and other fields and has a wide and good application prospect.

Description

technical field [0001] The invention belongs to the field of simulated enzymes, in particular to the use of small molecules containing hydrogen ions as peroxidase-like catalysts. Background technique [0002] Enzymes are biological catalysts and are closely related to life activities. Enzymes are composed of proteins, which are generally unstable to acid, alkali and heat, and are easily inactivated and denatured, which limits their wide application. The study of mimetic enzymes that imitate the molecular recognition of natural enzymes for substrates and efficient catalysis is a very meaningful research field, and it is also an important topic in many fields in the past ten years. Horseradish peroxidase is a commonly used tool enzyme, so it is of great significance to the research and development of peroxide mimetic enzymes. Contents of the invention [0003] The purpose of the present invention is to overcome the disadvantages and deficiencies of the prior art, and provi...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/76
CPCG01N21/76G01N21/78
Inventor 栗瑞敏邓毛程李静陈维新
Owner GUANGDONG IND TECHN COLLEGE
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