46 results about "Penicillin Antibiotic" patented technology

The invention provides a high-purity antibiotic medicinal compound, and in particular relates to a method for refining beta-lactam penicillin antibiotic medicinal compounds. In the method, an acid-base pH method and a macroporous absorption resin method are effectively combined; and beta-lactam penicillin antibiotic medicinal compounds are refined and purified under specific process parameters to prepare over 99.9 percent high-purity beta-lactam penicillin antibiotic medicinal compounds with yield over 90 percent, so that unexpected purification effect is achieved. The high-purity beta-lactam penicillin antibiotic medicinal compounds are prepared by the method, so that the stability of corresponding preparations thereof and clinical application effect can be improved. The method is simple, has low cost, is easily for operation, and is applicable to large-scale industrial production.

The invention relates to a detection kit and a detection method of raw milk and milk products, in particular to a detection kit and a detection method for beta-lactamase in raw milk and milk products. At present, no diary corporation in China can carry out production with the raw milk completely supplied by self-owned farms. All diary corporations need to purchase raw milk from dairy farmer to some extent. Thus, the detection of imparities and antibiotics becomes extremely important. The detection method adopts a standard strain of bacillus stearothermophilus absolutely sensitive to penicillin antibiotics, uses the specificity of sulbactam to inhibit the activity of the beta-lactamase, adds ampicillin as a reference and contrasts inhabitation zones of a sample with a beta-lactamase inhibitor and a sample without the inhibitor to detect the antibiotics. The detection kit comprises a sterilized culture dish with a vacuum package, a beta-lactamase standard product, a freeze-drying cillin bottle containing ampicillin and ampicillin and sulbactam, a blank filter paper sheet and a diluent. The detection kit and the detection method have the advantages of low cost, convenient and quick operation and high sensitivity.

The invention discloses a penicillin antibiotic aptamer obtained by the screening technology for an improved aptamer without a fixed point target substance in the field of screening a penicillin antibiotic aptamer without a fixed point target substance, and an application thereof. The unfixed nucleic acid is eluted through a fixed oligonucleotide library; the parent nucleus 6-APA (6-aminopenicillanic acid) of the penicillin antibiotic is added in positive screening to act on the fixed nucleic acid; after eluting the nucleic acid molecules which can be combined with 6-APA, PCR (polymerase chain reaction) amplification is directly performed for next screening; and other antibiotics are added in negative screening, and the nucleic acid molecules in non-specific binding with the penicillin antibiotics are removed by elution. Through multiple rounds of positive and negative screening, 10 nucleic acid aptamers with high specificity and strong affinity with 6-APA are obtained; and the nucleic acid aptamer with a stable secondary structure is selected for developing a nucleic acid aptamer sensor for detecting 6-APA.

The present invention relates to a slow-released preparation containing beta-lactamase inhibitor. Said slow-released preparation can be made into antibiotic slow-released injection or slow-released implant preparation. Said injection is formed from slow-released microsphere and solvent, said slow-released microsphere contains slow-released auxiliary material and beta-lactamase inhibitor with antibacterial effective dose and penicillin antibiotics, the solvent is special solvent containing suspension adjuvant of carboxymethyl cellulose sodium, etc. its viscosity is 100 cp-3000 cp (20 deg.C-30deg.C); the slow-released auxiliary material is selected from EVAc, polylactic acid copolymer, sebacic acid copolymer, albumin glue and glatin, etc. The slow-released implant preparation is prepared by using slow-released microsphere or adopting melting process. Said invention also provides its application range and can obtain obvious therapeutic effect for curing various infective diseases.

The present invention relates to a compound injection preparation formed from probenecid sodium and benzylpenicillin antibiotics, belonging to the field of chemical pharmaceutical technology. The benzylpenicillin antibiotics include amoxicillin sodium and piperacillin sodium. The amoxicillin sodium and piperacillin sodium are mixed according to ratio of 1:3 and 1:5 through a conventional preparation process.

The invention relates to a method for removing residual penicillin antibiotics in biological medicine waste residues. The method comprises the following steps that (1) an activating agent is added into the biological medicine waste residues containing the penicillin antibiotics, size mixing and dispersing are carried out, then waste residue slurry is obtained; and (2) a catalyst and an oxidizing agent are added into the waste residue slurry obtained in the step (1), and after the degradation reaction, the residual penicillin antibiotics in the biological medicine waste residues is removed. Compared with the prior art, the residual antibiotics can be rapidly and efficiently removed by 99% and more by the method, moreover, the penicillin antibiotics is degraded into other substances which are non-toxic and have no pesticide effect, the obtained waste residues are solid, is convenient to store and transport, and can be applied to production of fertilizers and soil conditioners, the development concept of green coordination and sustainable development is met, and the requirements of energy conservation and environmental protection advocated by China are met.

The invention discloses a making method of penicillin antibiotic disodium tikaxilin salt, which comprises the following steps: adding diluent in the tikaxilin acid solution at -5-50 deg. c; decoloring through active carbon; filtering; adding salting agent; stirring; culturing crystal at -10-50 deg. c until the crystal is evolved completely; filtering; washing; drying in the vacuum under 20-80 deg. c; obtaining the product.

The invention relates to a method for separation and enrichment of penicillin antibiotics in water. The method comprises the following steps of adjusting a pH value of a water sample to 2-4 by an acid; filtering the water sample to obtain a pretreated water sample; activating an HLB solid-phase extraction column by acetone, methanol and a formic acid aqueous solution containing ammonium acetate successively; adding ultrapure water to keep a resin in the HLB solid phase extraction column in an activated state; passing the water sample through the column; drying the HLB solid-phase extraction column under the protection of nitrogen after enrichment is finished; eluting with an eluent; collecting the eluent; blow-drying under a nitrogen flow; adding acetonitrile to dissolve residual materials for detection; and detecting the concentrations of penicillin antibiotics quantitatively. The method is advantageous in that the pretreatment of the water sample is environment-friendly; and the method can be operated easily, has high enrichment coefficient, low detection limit and good repeatability, and can analyze the content of two trace penicillin antibiotics in a water environment rapidly and accurately.

The invention relates to a release injection which contains cefradine, wherein it is formed by release micro ball and dissolvent; the release micro ball contains release findings and penicillin antibiotic; the dissolvent is the special one contains sodium carboxymethyl cellulose as suspension agent; the viscosity is 100cp-3000cp (20Deg. C-30Deg. C); the release agent is selected from EVAc, polyphenyl, PLA, PLGA, sebacic acid polymer, protein pastern, and dope; the release micro ball can be made into release implant agent; the release implant agent and release injection is laid on bacteria stove or injected, to release the medicine for 5-30 days, to keep the effective medicine density and reduce the whole toxicity. The invention can be used to cure the infection caused by staphylococcus, streptococcus, etc.

The invention relates to a release injection which contains mycin antibiotic, wherein the invention is formed by release micro ball and dissolvent; the release micro ball contains release findings and penicillin antibiotic; the dissolvent is the special one contains sodium carboxymethyl cellulose as suspension agent; the viscosity is 100cp-3000cp (20Deg. C-30Deg. C); the release agent is selected from EVAc, polyphenyl, PLA, PLGA, sebacic acid polymer, protein pastern, and dope; the release micro ball can be made into release implant agent; the release implant agent and release injection is laid on bacteria stove or injected, to release the medicine for 5-30 days, to keep the effective medicine density and reduce the whole toxicity. The invention can be used to cure the infection caused by staphylococcus, streptococcus, etc.

The invention relates to a mouse monoclonal antibody cell strain for resisting both amoxicillin and ampicillin, belonging to the technical field of B cell hybridoma. The mouse monoclonal antibody cell strain for resisting the amoxicillin and the ampicillin has the preservation number of CGMCC NO.2674. The monoclonal antibody aiming at the amoxicillin has 100 percent of cross reaction with the ampicillin which also belongs to the penicillin antibiotics, and has no cross reaction with cefazolin of cephalosporin antibiotics. The invention can be used for rapidly detecting the residual condition of the amoxicillin and the ampicillin in animal-sourced food, and can be further used for developing an immunity detection product rapidly and accurately detecting the amoxicillin and the ampicillin in the animal-sourced food.

The invention relates to a compound inhalation preparation containing penicillin antibiotic and glucocorticoid and applications thereof. The preparation is capable of treating pulmonary bacterial infection with less dosage and has a certain curative effect on asthma and chronic obstructive pneumonia. The preparation is made into an dry powder inhalation dosage form, after a long period of preservation, the content of allergic matters namely penicilloic acid and penicillenic acid is not increased, so that the happening rate of allergy is largely reduced, when the preparation is used to treat asthma caused by non-microbial infection and chronic obstructive pneumonia.

The invention relates to acne-removing cream and a preparation method thereof. The acne-removing cream is prepared by mixing a mixture of lincomycin hydrochloride and lidocaine hydrochloride with a mixture of clindamycin and penicillin antibiotics at a certain proportion. The acne-removing cream provided by the invention has the advantages as follows: the acne-removing effect is enhanced while the cost is not increased, no pain is caused in the treatment process, no scar is left after treatment, skin allergy and inflammation does not occur, high safety is achieved, no toxic or side effect is realized, the acne-removing period is short, no relapses after curing is realized, patients can be fully cured and restored to health within a short time at a low cost, so as to be clear and refresh in skin and return to a normal life, dual curative effects on both recurrent acne and severe acne are achieved, and an obvious curative effect on skin problems like acne marks, acne and comedo and the like is also achieved.

The invention provides a multi-residual enzyme-linked immunoassay method used for antibiotics such as penicillins and the like, belonging to the technical field of immunoassay. The method obtains a polyclonal antibody by utilizing immunogen immunity synthesized by 6-amino penicillanic acid; the antibiotics such as the penicillins and the like are used as standard products and the conjugate of the 6-amino penicillanic acid and OVA is used as coating antigen, thus establishing the indirect ELISA method of antibiotics such as penicillins and the like in animal foods. The method provides a quick and high-efficiency detection means for the residual detection of the antibiotics such as penicillins and the like in the animal foods; as the polyclonal antibody is adopted, the expense is lower and the stability and repeatability are better; furthermore, the method has the advantages of simple predisposal, high sensitiveness, high precision and the like.

The invention discloses a sustained release injection containing mezlocillin, which consists of sustained release microspheres and menstruum, wherein, the sustained release microspheres contain sustained release excipient and penicillin antibiotics, the menstruum is special menstruum containing suspending agents such as sodium carboxymethyl cellulose and so on, and the viscosity is between 100 and 3000cp (at the temperature of between 20 and 30 DEG C); the sustained release excipient is selected from EVAc, polifeprosan, PLA, PLGA, decanedioic acid copolymers, albumin glue, gelatin and so on; and the sustained release microspheres can also be prepared into sustained release implant. The sustained release implant and the sustained release injection are locally placed or injected on a bacteria focus, which can slowly release medicine at a local lesion for 5 to 30 days and apparently decrease the systemic toxicity of the medicine when the effective medicine concentration of the local lesion is effectively obtained and maintained. The sustained release injection has a remarkable and unique treatment effect on infections caused by staphylococcus, streptococcus, peptostreptococcus, acne propionate bacillus, enterobacter, tubercle bacillus, gonococcus or meningococcus and so on, particularly on local lesions such as chronic osteomyelitis, severe bedsore, refractory skin ulcer, diabetic foot and femoral head necrosis of diabetes, and various abscesses and so on.

The invention provides a preparation jointly using glycine and amoxicillin, and relates to a micromolecule substance, such as glycine. The glycine can be used for improving the sensitivity of bacteriato penicillin antibiotics, such as amoxicillin; the glycine and glucose can be used for improving the sensitivity of bacteria to penicillin antibiotics, such as amoxicillin. The preparation jointly using the glycine and the amoxicillin is prepared from the glycine and / or the glucose, and the penicillin antibiotics, such as amoxicillin. The preparation has the advantages that by adding the glycineand / or the glucose, the survival rate of edwardsiella tarda is obviously reduced when the edwardsiella tarda is treated by the amoxicillin, and the glycine and / or the glucose can improve the sensitivity of the edwardsiella tarda to the amoxicillin; after the glycine and / or the glucose is added, the survival rate of escherichia coli is obviously reduced when the escherichia coli is treated by theamoxicillin,, and the glycine and / or the glucose can improve the sensitivity of the escherichia coli to the amoxicillin.

The invention relates to a compound inhalation preparation containing penicillin antibiotic and applications thereof. The preparation in an inhalation dosage form is capable of treating pulmonary bacterial infection with less dosage and has a certain curative effect on asthma and chronic obstructive pneumonia. The preparation is made into an dry powder inhalation dosage form, after a long period of preservation, the content of allergic matters namely penicilloic acid and penicillenic acid is not increased, so that the happening rate of allergy is largely reduced, when the preparation is used to treat asthma caused by non-microbial infection and chronic obstructive pneumonia.

The invention relates to a bacteria culture bottle containing beta-lactamase and a preparation method thereof. The beta-lactamase capable of decomposing cephalosporins and penicillin antibiotics is added in the culture bottle to treat residual beta-lactamase antibiotics in blood specimens. The preparation method includes the following steps: preparing the beta-lactamase with corresponding dosage according to the types and dosages of the beta-lactamase antibiotics that child patients take, the plasma concentration of antibiotic peaks in corresponding package inserts and the proportioning requirements in the specification for beta-lactamase preparations; and simultaneously injecting the beta-lactamase and a clinical blood culture specimen into a clinically used bacteria culture bottle or a manually prepared bacteria culture bottle so as to obtain the bacteria culture bottle containing the beta-lactamase. Thus, the positive detection rate of blood culture can be increased, and time to positivity can be shortened.

The invention provides a ticarcillin sodium preparation method which has mild reaction conditions, less side reactions, less steps, and high controllability, and the method comprises the following steps: (1) allowing 3-thienylmalonic acid to react with N-hydroxysuccinimide in an organic solvent with the catalysis of DCC and DMAP to obtain an intermediate 1, performing separation and purification by column chromatography; (2) dissolving the intermediate 1 in dichloromethane, adding 6-APA and an alkali to react so as to obtain ticarcillin; (3) allowing ticarcillin to react with a salt forming agent to obtain ticarcillin sodium.

The invention relates to a preparation method of a penicillin antibiotic impurity. The penicillin antibiotic impurity has a structure represented by formula (I), and in the formula (I), R represents side chains of penicillin, mezlocillin, piperacillin sodium and flucloxacillin. The method comprises the following steps: (1) mixing penicillin antibiotic, beta-lactamase and water; (2) controlling thetemperature to be 37 DEG C or below; (3) purifying by preparative chromatography, and concentrating; and (3) mixing and reacting with an alcohol solvent. The method is simple to operate and short inreaction time, the purity of the product is 90% or above, and the product can be directly used as a reference substance to qualitatively and quantitatively research penicillin antibiotics, so that theproduct quality is effectively controlled.

A sustained release injection containing penicillins antibiotic consists of sustained release microsphere and dissolvant, wherein the sustained release microsphere contains sustained release auxiliary materials and penicillins antibiotic; the dissolvant is special dissolvant containing suspending agents such as sodium carboxymethyl cellulose, and the viscosity of the dissolvant is 100 to 3,000 cp (at a temperature of between 20 and 20 DEG C); the sustained release auxiliary materials are selected from EVAc, Polifeprosan, PLA, PLGA, sebacic acid copolymer, albumin glue and gelatin, etc.; the sustained release microsphere can also be made into a sustained release implantation agent. The sustained release implantation agent and sustained release injection can be partly placed or injected into a bacteria focus so as to slowly release medicine over local bacteria focus for 5 to more than 30 days, thereby obviously reducing whole-length toxicity while effectively obtaining and maintaining the effective drug concentration of local focus. The sustained release preparation has remarkable and unique therapeutic effects in curing infections caused by staphylococcus, streptococcus, peptostreptococcus, propionibacterium acnes, enterobacter, tubercle bacillus, gonococcus or meningococcus, etc., and local focuses in particular such as chronic osteomyelitis, severe bedsore, intractable skin ulcer, diabetic foot, femoral head necrosis and various abscesses.

A polymer containing a carboxyl group, a preparation method and an application thereof, a supported catalyst and a preparation method thereof and preparation methods of penem antibiotic intermediate are disclosed. The polymer has high rigidity and hardness, thus the mechanical properties of the polymer is effectively improved. Meanwhile, in the polymer, the carboxyl group is used as a main functional group, and is used as a carrier to prepare, by means of a coordination reaction between the carboxyl group and a heavy metal, a supported metal catalyst which has better connection stability between the metal and the polymer. The above two factors can improve the stability of the supported metal catalyst, such that the catalyst can be recycled without losing the catalytic activity. Meanwhile, loss of a heavy metal active ingredient and production cost can be reduced.

The invention relates to a mouse monoclonal antibody cell strain for resisting both amoxicillin and ampicillin, belonging to the technical field of B cell hybridoma. The mouse monoclonal antibody cell strain for resisting the amoxicillin and the ampicillin has the preservation number of CGMCC NO.2674. The monoclonal antibody aiming at the amoxicillin has 100 percent of cross reaction with the ampicillin which also belongs to the penicillin antibiotics, and has no cross reaction with cefazolin of cephalosporin antibiotics. The invention can be used for rapidly detecting the residual condition of the amoxicillin and the ampicillin in animal-sourced food, and can be further used for developing an immunity detection product rapidly and accurately detecting the amoxicillin and the ampicillin in the animal-sourced food.

The invention discloses a making method of penicillin antibiotic disodium tikaxilin salt, which comprises the following steps: adding diluent in the tikaxilin acid solution at -5-50 deg. c; decoloring through active carbon; filtering; adding salting agent; stirring; culturing crystal at -10-50 deg. c until the crystal is evolved completely; filtering; washing; drying in the vacuum under 20-80 deg. c; obtaining the product.

The invention relates to a novel method for preparing broad-spectrum penicillin antibiotic ticarcillin sodium. The method is characterized in that the method comprises the following steps of: (1) directly carrying out chlorination on 3-thiophenemalonic acid used as raw material and thionyl chloride in the presence of a catalyst, without protecting carboxyl, and chloridizing one carboxyl to obtiana compound (II); (2) carrying out condensation reaction with 6-aminopenicilanic acid (6-APA), controlling the reaction temperature and PH value, dripping the compound (II) and sodium bicarbonate solution, dispensing liquid after reaction, adjusting PH value to wash, adjusting pH value again to 2.0, extracting reaction solution, and drying to obtain a compound (IV); using sodium iso-octoate to salify, and refining crude products, to obtian the ticarcillin sodium salt.

The invention discloses a penicillin antibiotic appearance evaluation method which is as follows: determining appearance standards of a certain penicillin antibiotic by use of spectrophotometric method, and comparing the determined appearance standards with the absorbance of other batches of solutions of the penicillin antibiotic to evaluate wether the appearance of the penicillin antibiotic product of the to-be tested batch is qualified. A penicillin antibiotic appearance naked eye observation method is replaced by the penicillin antibiotic appearance evaluation method, the appearance standard is quantifiable, easy to control, objective and accurate, and does not change with time, place, personnel changes. The penicillin antibiotic appearance evaluation method has the advantages of low cost, high precision and high efficiency in the popularization and application in industrial production.

A sustained release preparation containing ticarcillin consists of sustained release microsphere and dissolvant, wherein the sustained release microsphere contains sustained release auxiliary materials and penicillins antibiotic; the dissolvant is special dissolvant containing suspending agents such as sodium carboxymethyl cellulose, and the viscosity of the dissolvant is 100 to 3,000 cp (at a temperature of between 20 and 20 DEG C); the sustained release auxiliary materials are selected from EVAc, Polifeprosan, PLA, PLGA, sebacic acid copolymer, albumin glue and gelatin, etc.; the sustained release microsphere can also be made into a sustained release implantation agent. The sustained release implantation agent and sustained release injection can be partly placed or injected into a bacteria focus so as to slowly release medicine over local bacteria focus for 5 to more than 30 days, thereby obviously reducing whole-length toxicity while effectively obtaining and maintaining the effective drug concentration of local focus. The sustained release preparation has remarkable and unique therapeutic effects in curing infections caused by staphylococcus, streptococcus, peptostreptococcus, propionibacterium acnes, enterobacter, tubercle bacillus, gonococcus or meningococcus, etc., and local focuses in particular such as chronic osteomyelitis, severe bedsore, intractable skin ulcer, diabetic foot, femoral head necrosis and various abscesses.

The present invention relates to a preparation method for molecular imprinted polymer which can get rid of the penicillin-like antibiotics in the fresh milk. The present invention belongs to the foodchemistry field. The preparation method lie in that template molecule, functional monomer, cross-linking agent and the initiator are mixed together to be oxygenated, thermal polymerized, grinded, cleaned and dried in vacuum to get molecular imprinted polymer. The present invention is simple and controllable. The required experimental devices are simple. The generated molecular imprinted polymer isof strong stability, long storage time, large adsorption capacity and fast adsorption speed. The counter- imprinted molecular is of strong memory and no toxin and any side effect. The molecular imprinted polymer can effectively get rid f the penicillin-like antibiotics in the fresh milk and can be used in the examination of penicillin-like antibiotics in the fresh milk. The present invention is of very broad prospects for development and application.

The invention belongs to the field of biotechnology and particularly discloses an application of glutathione reductase to binding of penicillin antibiotics. A sequence of the glutathione reductase is as shown in SEQ ID NO:1. The glutathione reductase ETAE_3367 is a new penicillin antibiotic binding protein and can be effectively applied to the detection of the penicillin antibiotics, the preparation of detection reagents and further research on effects and drug resistance mechanisms of the penicillin antibiotics.

The invention relates to a method for removing residual penicillin antibiotics in biomedical waste residues, comprising the following steps: (1) adding an activator to the biomedical waste residues containing penicillin antibiotics, and obtaining waste residue slurry after slurry mixing and dispersion; 2) Adding a catalyst and an oxidizing agent to the waste residue slurry obtained in step (1), and performing a degradation reaction to complete the removal of the residual penicillin antibiotics in the biomedical waste residue. Compared with the prior art, the present invention can quickly and efficiently remove more than 99% of residual antibiotics, and this method degrades penicillin antibiotics into other non-toxic and non-medicinal substances, and the waste residue obtained from the treatment is solid, which is convenient for storage It can be used for the production of fertilizers and soil conditioners, which conforms to the concept of green, coordinated and sustainable development, and meets the requirements of energy conservation and environmental protection advocated by the state.