41 results about "Microbial respiration" patented technology

Methods of treating a subterranean formation penetrated by a wellbore of a well, wherein the subterranean formation includes carbonate. The methods can include the following steps of: (1) optionally, fracturing the subterranean formation; (2) optionally, acidizing the subterranean formation with a Bronsted-Lowry acid; (3) treating the subterranean formation with an acid-producing microorganism, a nutrient for the microorganism, and, if needed, a suitable electron acceptor for respiration by the microorganism; (4) optionally, flushing the wellbore with a wash fluid to push the microorganism deeper into the subterranean formation and wash it away from the metal tubulars of the well; (5) preferably, shutting-in the well for a required incubation period for in-situ acid generation by the microorganism; and (6) preferably, after the shut-in, flowing back fluid from the subterranean formation into the wellbore.

The invention discloses a method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current. The method is characterized in that a paper-based micro-fluidic chip is prepared and then water pollutant biotoxicity is determined by the paper-based micro-fluidic chip. According to a microbial respiratory chain-based BQ system electrochemical detection principle, on the paper-based micro-fluidic chip of a screen printing PDMS hydrophilic microchannel and carbon paste three-electrode system, a steam trap of the hydrophilic microchannel is dredged by a small piece of a cellulose membrane so that liquid flow release time and flowing time are controlled and separation and cleaning of a nutrient solution and cells and cell incubation by pollutants are realized and thus the interference produced by current signal detection device anode current-based quantitative determination of pollutant toxicity is reduced. The method has the advantages of light quality, carrying convenience, low cost, one-step analysis, small sample volume and fast analysis speed and is suitable for biotoxicity detection in occasions of a remote area, field on-site water pollution, soil pollution, food safety and space carrying.

The invention relates to the field of machinery, and in particular provides a device for automatically continuously testing soil microbial respiration. The device comprises a constant temperature culture system, a CO2 initial concentration control and gas sampling system and a CO2 concentration analyzer. The device can adjust CO2 concentration and gas flow and flow velocity in the device at the beginning of a test according to requirements, and has a function of automatically switching different test specimens. By the device, the indoor soil microbial respiration can be quickly and continuously tested on line successfully, and the device can be widely applied to the related fields of soil carbon turnover, microorganisms and the like.

The invention provides a device for detecting toxicity of a water body. The device comprises a micro-organism culturing device and an electrochemical detection device communicated with the micro-organism culturing device, wherein the electrochemical detection device comprises an electrochemical detection tank, a working electrode, a reference electrode, a counter electrode and a constant-temperature device; the working electrode, the reference electrode and the counter electrode are arranged in the electrochemical detection tank; and the constant-temperature device is used for keeping the electrochemical detection tank at constant temperature. The invention further provides a method for detecting the toxicity of the water body. The detection device provided by the invention is used for detecting inhibition of toxic substances in water on micro-organism respiration through the electrochemical detection device, so that the toxicity of the water body is detected; the detection device is simple in structure; furthermore, concentration correction is not required; a detection result cannot be influenced; simultaneously, the detection device provided by the invention is free from influence of turbidity and colour of the water body, high in detection sensitivity and good in repeatability; and a water sample to be detected is detected by taking a micro-array electrode as a working electrode, so that detection current can be increased, thus, the detection sensitivity is increased.

The invention relates to a preparation method of calcium carbonate and particularly relates to a preparation method of nano activated calcium carbonate. The method comprises the steps of firstly preparing slurry from activated carbon, adsorbing dopamine into an activated carbon pore structure in a weak alkaline environment, carrying out oxidative autopolymerization under the action of dissolved oxygen in water to form a three-dimensional network structure in the pore structure, loading a microorganism obtained through fruticose lichen culture by using pretreated activated carbon, adding the microorganism-loaded activated carbon into a calcium chloride solution, chelating an organic matter with negative charges on the cell surface of the microorganism and calcium ions in the solution, and continuously generating carbon dioxide through the respiratory action of the microorganism, so that the concentration of carbonate ions in a surrounding solution of the microorganism is continuously increased, the concentration of the carbonate ions and calcium ions in activated carbon pores is increased, calcium carbonate crystal precipitate formed by combination of the carbonate ions and the calcium ions is separated out and adsorbed by the activated carbon and is burnt to remove the activated carbon, and the nano activated calcium carbonate is prepared through activating treatment of a silane coupling agent.

The invention discloses a freeze-dried fruit yoghourt block and a preparation process thereof. The freeze-dried fruit yoghourt block comprises the following raw materials in parts by weight: 5-10 parts of yoghourt, 1-2 parts of saccharide, 1-2 parts of milk powder, 1-2 parts of whey powder, 1-2 parts of citric acid, 0.1-0.5 part of starch and 0.1-0.2 part of pectin. According to the invention, fruit grains are mixed with the yoghourt, and processing procedures of quick freezing and freeze drying are carried out, so that the water content in the freeze-dried fruit yoghourt block is reduced, therespiration rate of microorganisms in raw materials is reduced, and the shelf life of the raw materials is prolonged; the quick drying procedure enables the water loss rate in the raw materials to reach 80%-90%, so that the loss rate of nutrient components is reduced; the added water is filtered and is subjected to boiling treatment, and the sterilization effect is high; and meanwhile, macromolecular saccharides in fruits are converted into small molecular substances which can be absorbed more easily, so that the absorption rate of the nutrient components of human bodies is increased.

The invention discloses a preparation method of recycled aggregate concrete, and belongs to the technical field of preparation of building materials. According to the method, calcine lime is dissolvedin water during fermentation, heat release provides temperature required for fermentation, produced calcium hydroxide forms an alkaline environment in the water, silicon dioxide, the main component in rice hull ash, is transformed into silicate to be filled into holes of aggregate filter residues, and in addition, microbial respiration produces silicon dioxide during fermentation. The method hasthe advantages that firstly, produced recycled aggregate is low in porosity, and absorption to water can be reduced; secondly, as methoxyl in methoxytrimethyl silane on the surfaces of the aggregate filter residues is easy to hydrolyze and can be in reaction with ammonia gas to produce methyl ammonia alkane substance, the methyl ammonia alkane substance can perform substitution reaction with potassium ion in the rice hull ash, the recycled aggregate concrete has excellent waterproof effect, and the absorption to water by concrete is reduced, so that scaling of the recycled aggregate concrete caused by freeze thawing and weathering is reduced, the service life is prolonged, and the recycled aggregate concrete has a wide application prospect.

The invention discloses a microbe breathing oxygen consumption monitoring-based water toxicity on-line early-warning device and method and relates to the technical field of water toxicity on-line early warning. According to the method, microbes are cultured in a bioreactor, through control of conditions, microbe growth is in a dynamic balance state so that a dissolved oxygen value in the bioreactor is in a stable state, when water contains toxic pollutants, microbe respiration intensity is changed so that a dissolved oxygen level in the bioreactor is changed, and through acquisition of change of the dissolved oxygen level, water toxicity pollution on-line early warning is realized. The device realizes on-line monitoring of a dissolved oxygen level in the bioreactor only by a dissolved oxygen sensor, is used simply, has good maneuverability, does not need special reagent or apparatus, is maintained simply and has a low operation cost.

The invention discloses a furrow covering cultivation method capable of increasing natural rainfall infiltration, storage and utilization and aims to solve the problems that the partial pressure of carbon dioxide of soil in a film in the whole plastic-film mulching technology is excessively high, deep root systems and microorganisms have breathing difficulties, and soil nutrients are low in circulation rate.The method includes: mulching and ridging are performed at the same time, mulching is performed on the ridges, an open ditch and a blind ditch are arranged at the connection of two ridges at interval, the blind ditch is a rain collecting ditch, and the open ditch is covered by crop straw.The method has the advantages that the defect that the whole covering on double ridges and planting in catchment furrows excessively consumes deep moisture and the moisture on the film evaporates easily, and the method is significant to soil moisture recovering and sustainable development of soil water.

The invention discloses a preparation method of a thermal infrared shielding coating material and belongs to the field of coating material synthesis. According to the invention, quick lime and gypsum are adopted to erode coal ash microspheres under the heating condition, so that partial defect is formed on the surface to be activated, and then the coal ash microspheres are sintered together with magnesite at high temperature to produce porous stone powder, and the porous stone powder is mixed with a metallic oxide and subjected to earthworm fermentation, then is uniformly mixed, and then is uniformly mixed with acetic ether and polyurethane resin, thus obtaining the finished product. According to the invention, the spherical structure of hollow glass beads in coal ash are used for reflecting sunlight, so as to reduce sunlight absorption; and after earthworm fermentation, the microbial respiratory action in earthworm enables the interior of the microspheres to be rich in air, and an effective heat barrier is formed in the coating, so that the cooling effect is greatly improved, and after combination with sintering of magnesite, the expansion coefficient is reduced, the thermal shock resistant stability is improved, and the service life is prolonged.

Liquid chemical compositions are disclosed for anaerobic biodegradation, detoxification, and transformation of toxic organic and inorganic compounds in a contaminated geologic media under reducing conditions, including, but not limited to, denitrifying, manganese-reducing, iron-reducing and sulfate-reducing conditions. One such liquid chemical composition includes sodium nitrate in the range of one-fifth (0.2) to four (4) pounds per gallon of the chemical composition; sodium hexametaphosphate or other biologically hydrolyzable ring or linear polyphosphate in the range of one twentieth (0.05) to five (5) pounds per gallon of the chemical composition; a surfactant in the range of 0.01% to 10% by volume of the chemical composition; and a diluent in the form of water.A method is disclosed for anaerobic biodegradation, detoxification, and transformation of toxic organic and inorganic compounds in a contaminated geologic media comprising the steps of pressurizing one or more storage tanks containing a chemical composition using an inert carrier gas; connecting a plurality of quick disconnect valve couplings to one or more pressurized storage tanks; connecting a well to an automatic ball valve for supplying the chemical composition and the inert carrier gas through the well to the contaminated geologic media; and opening and closing the automatic ball valve to dispense the chemical composition and the inert carrier gas under pressure through the well to the contaminated geologic media. Methods are also disclosed for alternating the cycles of redox potential and the predominant microbial respiration pathway within the contaminated geological media.

A method for the phenotypic identification of microorganisms is provided. The method is based on the evaluation of the effects of various compounds (effectors) on the respiratory cycle activity of microorganisms. Measurements are based upon the ability of the microorganism to transport electrons to an external chemical oxidant (a mediator) that is added to the microorganism sample. The mediator interacts with the terminal components of the respiratory pathway and the extent of its consumption is related to the ability of the microorganism to respire. The consumed mediator is subsequently measured electrochemically. Electrochemical signals which are generated in the presence or absence of an effector can be used to generate a signal pattern that is unique to an organism and can be used for identification.

The invention discloses a method for assessing robustness of an activated sludge system based on a reinforcing effect of calcium. The method comprises the following steps: 1) taking mud from a sewage disposal plant, conducting inoculating and habituated culture, implementing cooling and water quality impact experiments and adding CaCl2, and determining microbial respiratory atlases before and after adding the calcium; determining OURs, OURq, OURe, OURen and OURenc in accordance with the obtained microbial respiratory atlases; in accordance with the obtained parameters, acquiring an autotrophic bacterium respiratory rate OURn, so as to judge the robustness of the system; and subsequently, acquiring an endogenous respiratory ratio RI, so as to judge whether system microorganisms are impacted or not. The method provided by the invention is conducive to risk assessment and risk control of the sludge disposal plant; the method can optimize running of the sludge disposal plant; and the method has a certain guiding significance for operation management of the sludge disposal plant.

The invention discloses a soil water-retaining agent and a preparation method thereof, which belong to the field of crops production. The method adopts chitosan as a first layer network structure, sodium ethylene sulfonate, 2-diethylaminoethyl methacrylate and the like are added to a network structure, the moisture can be better locked, the respiratory action of microorganisms in the soil is always present, and can assist the water-retaining agent for long-term moisture preservation; in the first layer network structure, arbuscular mycorrhizal fungi and cyanobacteria are simultaneously added and mixed, a microbial respiratory responsive additive and carbon dioxide are acted, soil activity is activated, the microbe growth is stimulated, and nutrient can be provided; zeolite is modified by cetyl pyridine bromide, the water-absorption activity is increased, and moisture adsorption quantity is increased, at the same time, the mechanical properties of the gel are enhanced and not lost by the impact of external force, and the water retention performance is stably exerted. The soil water-retaining agent solves the problems of insufficient nutrition to farmland and short water-retention time efficiency of the current water-retaining agents.

The invention relates to the technical field of food microorganism detection and discloses a device and method for detecting the total number of colonies in foods. The device comprises a reaction chamber, an electric signal amplifier, an electric signal data acquisition card and an electric signal processor. A proton exchange membrane is arranged in the reaction chamber. The reaction chamber is divided into an anode reaction chamber and a cathode reaction chamber through the proton exchange membrane. A reaction system and electrodes are arranged in each one of the anode reaction chamber and the cathode reaction chamber. The electric signal amplifier is respectively connected to the electrodes in the anode reaction chamber and the cathode reaction chamber. The electric signal data acquisition card is respectively connected to the electric signal amplifier and the electric signal processor. The device and method utilize the principle of electrochemistry and utilizes electrons produced bymicrobial respiration as detection objects. Through the correlation curve of voltage values and the total number of colonies, which is drawn according to the known concentration of microbial broth, the total number of colonies in the sample are obtained in 30min and accuracy is 95% or more.

The invention relates to the technical field of soil respiration measurement, in particular to an indoor soil culture and soil respiration measuring system. The indoor soil culture and soil respiration measuring system comprises an air inlet system, a first control valve, a CO2 filtering device, a soil culture system, a second control valve, a measurement analyzer and a main control computer, wherein the air inlet system is connected with the soil culture system through the first control valve, the CO2 filtering device is connected with the first control valve, the soil culture system is connected with the measurement analyzer through the second control valve, and the main control computer is in signal connection with the first control valve, the second control valve and the measurement analyzer. By means of the indoor soil culture and soil respiration measuring system, it can be achieved that CO2 released when indoor soil microorganisms respire is continuously automatically measured in real time, and research and development requirements of current indoor soil culture measurement are met.

A method of using pure oxygen in microbial fermentation processes and wastewater treatment processes, in which the pure oxygen is produced by a pressure swing adsorption (PSA) process from a gas mixture of air and unused oxygen-containing recycled off-gas from microbial processes which uses oxygen-containing gas in a liquid or gas state. When pure oxygen with a purity of more than 90% is used in microbial processes, microbial productivity can be about 5 times as increased as it is that in the conventional method using air with an oxygen content of 21%, and the volume of an active sludge tank in wastewater treatment processes can be reduced to at most ⅕ of the volume as compared to the conventional method. Also, the off-gas from such pure oxygen processes has a high oxygen content of about 70-80%. Thus, when the off-gas is recycled with air for the supplement of oxygen consumption, the gas mixture with an oxygen content of about 40% is used as feed gas for the PSA process, and CO2 generated by microbial breathing is also removed. Thus, the volume of a PSA column and an air compressor for supplying the feed gas can be reduced to less than ½ of the volume as compared to that of the use of air.

A method for the phenotypic identification of microorganisms is provided. The method is based on the evaluation of the effects of various compounds (effectors) on the respiratory cycle activity of microorganisms. Measurements are based upon the ability of the microorganism to transport electrons to an external chemical oxidant (a mediator) that is added to the microorganism sample. The mediator interacts with the terminal components of the respiratory pathway and the extent of its consumption is related to the ability of the microorganism to respire. The consumed mediator is subsequently measured electrochemically. Electrochemical signals which are generated in the presence or absence of an effector can be used to generate a signal pattern that is unique to an organism and can be used for identification.

The invention discloses a sewage filtration microbiological comprehensive treatment system and a sewage treatment method thereof. The system comprises a filtering structure and a microbiological sprinkling structure, the filtering structure is arranged in the middle of the top end of a treatment tank, the top of the filtering structure is exposed from the water surface, the top of the filtering structure is communicated with a water inlet pipe, filtered sewage flows out of the filtering structure to the periphery, and the microbiological sprinkling structure sprays a microorganism bacterium liquid onto a rough-cut elastic filler net structure in the middle of the treatment tank to be in contact with filtered sewage. The sewage filtration microbiological comprehensive treatment system and an action method thereof can effectively and efficiently filter sewage and consume organic matters through microbial respiration to achieve a treatment effect.

The invention provides a device for detecting toxicity of a water body. The device comprises a micro-organism culturing device and an electrochemical detection device communicated with the micro-organism culturing device, wherein the electrochemical detection device comprises an electrochemical detection tank, a working electrode, a reference electrode, a counter electrode and a constant-temperature device; the working electrode, the reference electrode and the counter electrode are arranged in the electrochemical detection tank; and the constant-temperature device is used for keeping the electrochemical detection tank at constant temperature. The invention further provides a method for detecting the toxicity of the water body. The detection device provided by the invention is used for detecting inhibition of toxic substances in water on micro-organism respiration through the electrochemical detection device, so that the toxicity of the water body is detected; the detection device is simple in structure; furthermore, concentration correction is not required; a detection result cannot be influenced; simultaneously, the detection device provided by the invention is free from influence of turbidity and colour of the water body, high in detection sensitivity and good in repeatability; and a water sample to be detected is detected by taking a micro-array electrode as a working electrode, so that detection current can be increased, thus, the detection sensitivity is increased.

The invention provides an automatic microbial respiration rate measuring device, and relates to the field of instruments for scientific research on agriculture and the ecological environment, aiming at solving the problem that in the prior art, the microbial respiration rate can not be automatically and continuously measured on a large scale. The automatic microbial respiration rate measuring device comprises at least one culture flask, a multi-channel control system, a carbon dioxide analyzer and a data acquisition unit, wherein the multi-channel control system comprises at least one channel,and the channels are respectively connected with the culture flasks in a corresponding way; the channels are used for obtaining gas to be tested in the culture flasks; the carbon dioxide analyzer isconnected with the multi-channel control system and is used for measuring the concentration data of carbon dioxide in the gas to be tested; the data acquisition unit is connected with the carbon dioxide analyzer, and is used for acquiring the concentration data of the carbon dioxide and determining the microbial respiration rate according to the concentration data of the carbon dioxide.

The invention discloses a method for analyzing breathing intensity of soil microorganisms in Bt rice plantation and straw returning. The method comprises the following steps of: mixing an acquired soil sample and a glucose solution uniformly in a sealed container, culturing for 7 and 24 hours respectively, and measuring the amount of CO2 generated by each gram of soil sample by using gas chromatography, wherein the amount of the CO2 respectively represents the breathing intensity of the soil microorganisms in Bt rice plantation and straw returning and the capacity of soil microorganisms of utilizing glucose. The invention has the advantages that: the method is simple and quick; and most importantly, the amount of CO2 generated in 7 hours and 24 hours can be used for identifying the influence and the restoring force of Bt rice plantation and straw returning on the soil microorganisms.

The invention discloses a portable apparatus for measuring respiration of microorganisms in soil. The apparatus comprises a cover body (1), a hollow housing (3) and a metal inserting cylinder (6); a cover body (1) is tightly connected to the upper end of the hollow housing (3) through a screw thread joint (2), the lower end of the hollow housing (3) is tightly connected to the upper end of the metal inserting cylinder (6), and the inner wall of the hollow housing (3) is provided with a detection liquid bath (4) and a clean water tank (5). The apparatus is convenient to carry and has a low price, a gas source is provided for detection liquid in an enclosed environment, qualitative measurement for respiration of microorganisms in soil is realized, so that abundance results of microorganisms in soil are obtained.

The invention relates to the technical field of food microorganism detection and discloses a device and method for detecting the total number of colonies in foods. The device comprises a reaction chamber, an electric signal amplifier, an electric signal data acquisition card and an electric signal processor. A proton exchange membrane is arranged in the reaction chamber. The reaction chamber is divided into an anode reaction chamber and a cathode reaction chamber through the proton exchange membrane. A reaction system and electrodes are arranged in each one of the anode reaction chamber and the cathode reaction chamber. The electric signal amplifier is respectively connected to the electrodes in the anode reaction chamber and the cathode reaction chamber. The electric signal data acquisition card is respectively connected to the electric signal amplifier and the electric signal processor. The device and method utilize the principle of electrochemistry and utilizes electrons produced bymicrobial respiration as detection objects. Through the correlation curve of voltage values and the total number of colonies, which is drawn according to the known concentration of microbial broth, the total number of colonies in the sample are obtained in 30min and accuracy is 95% or more.

The invention discloses a microbial fertilizer for improving activity of functional microbes and a preparation method of the microbial fertilizer, and belongs to the field of preparation methods of biological fertilizers. The method comprises the following steps: uniformly stacking organic waste, and performing ventilation fermentation to obtain a decomposed organic fertilizer; performing drying naturally, placing the dried material in a drying room, and performing sterilization at a high temperature; adding the functional microbes, a nutrient auxiliary agent and a conditioning agent, performing uniform adsorption, adjusting the humidity, and performing secondary fermentation; and performing granulation on the finished-product microbial fertilizer, performing air drying, and performing packaging. According to the method provided by the invention, after the sterilization operation is completed, the secondary fermentation is performed, inoculation is performed by using the functional microbes, so that a quantity of impurity microbes is reduced, and the dominant position of the functional microbes is improved; the nutrient auxiliary agent is added into the organic fertilizer, so thatthe survival activity of the microorganisms is improved, improvement of activity of the microorganisms is facilitated, respiratory metabolism to release heat of the microorganisms is promoted, and decomposition of the organic fertilizer is slowed down; and the method solves the problem that functional microbes of a current microbial fertilizer have a poorer survival effect and the microbial fertilizer has a shorter shelf life.

The invention discloses an automatic collecting, replacing and sampling device for soil microorganism breathing gas. The device comprises an incubator, a vacuum pump, an air compressor, a negative pressure controller, a three-way balance pressure valve group, a three-way gas collection switching valve group, an automatic controller, at least two two-way balance pressure valve groups and two-way gas collection valve groups, culture bottles are communicated with gas inlets of two-way gas collection valves in the two-way gas collection valve groups in a one-to-one correspondence manner; gas outlets of two-way gas collection valves in the same group are communicated with independent gas collection ports of the same three-way gas production switching valve; a shared exhaust port of the three-way gas production switching valve group is communicated with a gas outlet of a ventilation three-way valve, a shared gas inlet of the three-way gas production switching valve group is communicated witha sample injection gas pipe, the sample injection gas pipe is communicated with a gas inlet of a sample injection three-way valve, and a gas outlet of the sample injection three-way valve is used forbeing communicated with a gas analyzer. According to the invention, the automatic collection, replacement and sample introduction of the sample gas can be realized, so that leakage and cross contamination of the sample gas are prevented, and the online continuous monitoring of the sample gas is ensured.

A rapid detection of microbial CO 2 A gel test paper for respiration rate and a using method thereof belong to the field of environmental chemistry and microbiology. The main components of the gel test paper include NaOH base, acid-base indicator, glycerin and agar, etc., with two test ranges of high range and low range, which can be used for CO 2 Respiration rate detection. The results of the gel test paper are highly readable. By comparing with the five color indicator cards of yellow, orange, red, deep red and purple, the respiration of the compost or soil can be quickly identified, and it can also indicate the five levels of microbial activity or maturity stage. The test paper of the present invention has good sensitivity and high accuracy, and the color change range of the high-range test paper matches 1-50 mg of CO 2 Respiration rate, low range test strip color change range to match 0.1‑5mg of CO 2 Respiration rate; the method of use is simple and fast, and the sample test can be completed within 30 minutes without the need for expensive testing instruments.

The invention relates to the field of machinery, and in particular provides a device for automatically continuously testing soil microbial respiration. The device comprises a constant temperature culture system, a CO2 initial concentration control and gas sampling system and a CO2 concentration analyzer. The device can adjust CO2 concentration and gas flow and flow velocity in the device at the beginning of a test according to requirements, and has a function of automatically switching different test specimens. By the device, the indoor soil microbial respiration can be quickly and continuously tested on line successfully, and the device can be widely applied to the related fields of soil carbon turnover, microorganisms and the like.

The invention discloses a device for continuous measurement of breathing delta13C of soil microorganisms. The device comprises a sampling system, an analytic system and a control system, wherein the sampling system is used for extracting a gas in a culture bottle and conveying the gas to the analytic system; the analytic system uses a calibration gas to perform calibration so as to obtain contents of CO2 and delta13C; and the control system is used for receiving data obtained by the analytic system, and controlling the sampling system. According to the device disclosed by the invention, by establishing an in-situ cultivation system with 16 incubators, continuous measurement and system integration can be achieved, functions of automatic sample injection and analysis are formed, and isotope ratios of dalta13C-CO2 and CO2 concentrations in the incubators can be measured continuously (0.3Hz); moreover, the device disclosed by the invention has a programmable temperature control function and a function of meeting simulation of a complex situation, and can use field experience for reference to establish an online calibration system and ensure the test accuracy, so that commercial mass production and full automatic operation can be achieved, the labor intensity can be reduced, and the device is quick and convenient to use.