Device and method for detecting toxicity of water body

A detection device and detection method technology, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of high detection cost, false positives, false negatives, etc., to improve detection sensitivity, increase detection current, and detect The effect of high sensitivity

Active Publication Date: 2012-06-27
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

International ISO11348-3 stipulates that the deep-sea luminescent bacteria V.fischeri is used as the test organism. In order to balance the osmotic pressure, the test must be carried out under high salinity conditions, which may cause changes in the properties of some chemicals in the sample, such as, for low concentrations For metal samples, salinity correction will lead to false negative results; for some samples with low solubility, such as phenol, it will lead to false positive results due to the precipitation of toxic substances
Moreover, this method uses fluorescence detection, and the detection signal is easily affected by the turbidity of the water body, so it is not suitable for turbid and colored samples
In addition, luminescent bacteria are not ubiquitous microorganisms in nature, and it is difficult to obtain and preserve them. The price of commercial luminescent bacteria is relatively expensive, resulting in high detection costs.

Method used

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  • Device and method for detecting toxicity of water body
  • Device and method for detecting toxicity of water body
  • Device and method for detecting toxicity of water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] by figure 1 The detection device shown, according to the following methods for water toxicity detection:

[0115] Step 1: Add Escherichia coli E.coli stored in a phosphate buffer solution with a pH of 0.2 mol / L and a pH of 7.0 into the electrochemical detection cell 21, then add the electron mediator potassium ferricyanide, and dilute with deionized water to 1 ml to make the final concentration of E.coli OD 600 is 6, the concentration of potassium ferricyanide is 45mmol / L; the electrochemical detection cell 21 is kept at a constant temperature of 20°C, and 20 pieces of diameter 25μm, passing through 0.5μm α-Al 2 O 3 The microarray electrode composed of the treated palladium microelectrodes is the working electrode, the Ag / AgCl electrode is the reference electrode, and the coiled platinum wire is the counter electrode; through an external electrochemical workstation CHI660, a +0.45 V bias for 10s, record the current value of the 10s as i 0-no-tox ;

[0116] Step 2: ...

Embodiment 2

[0122] The experiment is carried out according to the method and steps of Example 1, the difference is that 25 branches with a diameter of 20 μm and passing through 0.5 μm of α-Al 2 O 3 The microarray electrode composed of the treated gold microelectrodes was the working electrode, and arsenic ion solutions with concentrations of 20mg / L, 30mg / L, 50mg / L, 80mg / L and 100mg / L were prepared respectively. The results are shown in image 3 , image 3 The curve diagram of the concentration and the current inhibition rate provided in Example 2 of the present invention, by image 3 It can be seen that the IC50 value of arsenic ions is 92 mg / L.

Embodiment 3

[0124]The experiment is carried out according to the method and steps of Example 1, the difference is that 30 pieces with a diameter of 25 μm and passing through 0.5 μm of α-Al 2 O 3 The microarray electrode composed of the treated platinum-nickel alloy microelectrode is the working electrode, the electrochemical detection cell 21 is kept at a constant temperature of 25°C, and the BOD seed is used as the test organism, and the concentrations are respectively 2.5mg / L, 5mg / L, 7.5mg / L, 12.5mg / L, 15mg / L, 20mg / L, 25mg / L, 30mg / L and 35mg / L 3,5-dichlorophenol solution, see the results Figure 4 , Figure 4 The graph of the concentration and the current inhibition rate provided in Example 3 of the present invention is represented by Figure 4 It was found that the IC50 value of 3,5-dichlorophenol was 92 mg / L.

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Abstract

The invention provides a device for detecting toxicity of a water body. The device comprises a micro-organism culturing device and an electrochemical detection device communicated with the micro-organism culturing device, wherein the electrochemical detection device comprises an electrochemical detection tank, a working electrode, a reference electrode, a counter electrode and a constant-temperature device; the working electrode, the reference electrode and the counter electrode are arranged in the electrochemical detection tank; and the constant-temperature device is used for keeping the electrochemical detection tank at constant temperature. The invention further provides a method for detecting the toxicity of the water body. The detection device provided by the invention is used for detecting inhibition of toxic substances in water on micro-organism respiration through the electrochemical detection device, so that the toxicity of the water body is detected; the detection device is simple in structure; furthermore, concentration correction is not required; a detection result cannot be influenced; simultaneously, the detection device provided by the invention is free from influence of turbidity and colour of the water body, high in detection sensitivity and good in repeatability; and a water sample to be detected is detected by taking a micro-array electrode as a working electrode, so that detection current can be increased, thus, the detection sensitivity is increased.

Description

technical field [0001] The invention belongs to the technical field of water body detection, and in particular relates to a detection method for detecting water body toxicity and a water body toxicity detection method. Background technique [0002] With the development of human production activities, a large number of chemicals continue to enter the water environment and become the main source of water pollution. Most of these chemical pollutants are not the constituents of the human body, nor the nutrients required by the human body or the substances necessary to maintain normal physiological functions, but they can come into contact with the human body and enter the human body to produce certain biological effects, such as when toxic and harmful chemicals are used. Entering the human body through the amplification effect of the food web may endanger human health and even life. In addition, chemical pollution in the water body will also harm the organisms in the water body...

Claims

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Application Information

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IPC IPC(8): G01N27/416
Inventor 董绍俊雍达明刘畅翟俊峰
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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