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A rapid detection of microbial co 2 Gel test paper for respiration rate and method of use thereof

A respiration rate and microbial technology, applied in the field of environmental chemistry and microbiology, can solve the problems of long experiment time, unfavorable rapid measurement of respiration rate, reduced convenience and frequency of use, etc., to achieve good sensitivity, simple and fast use method, and accuracy high effect

Active Publication Date: 2021-08-10
DALIAN UNIV OF TECH
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  • Description
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AI Technical Summary

Problems solved by technology

Traditional CO 2 Respiratory testing, often using NaOH lye absorption retitration, CO 2 Methods and technologies such as infrared online detector method and gas chromatography require higher laboratory conditions, longer experiment time, and more skilled experimental technicians, which are not conducive to CO 2 Rapid measurement of respiration rate for CO reduction in soil and compost analysis 2 Convenience and Frequency of Respiration Rate Detection

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0018] The present invention will be further described below in conjunction with specific examples.

[0019] 1) Use the following formula to prepare high-range gel test paper: under the above concentration range, the optimal proportioning condition of the gel test paper is: with 1.5% agarose gel as the detection medium, each composition in every 100ml gel agarose The ingredients and their contents are: 12g NaOH base, 0.4g indicator alizarin yellow R, 0.6g indicator thymol blue, 4g glycerin. Cut the prepared gel into circular gel sheets with a diameter of 1 cm and a thickness of 0.1 mm, and attach them to a white plastic substrate to obtain a gel test paper. Take a 300mL transparent and sealable plastic tank, weigh 30g of compost samples into the plastic test tank, properly adjust the water content of the samples to 40-60%, insert the lower part of the gel test paper substrate on the compost samples, and keep the gel on the samples for 2- 3cm height, close the plastic jar, and...

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Abstract

A rapid detection of microbial CO 2 A gel test paper for respiration rate and a using method thereof belong to the field of environmental chemistry and microbiology. The main components of the gel test paper include NaOH base, acid-base indicator, glycerin and agar, etc., with two test ranges of high range and low range, which can be used for CO 2 Respiration rate detection. The results of the gel test paper are highly readable. By comparing with the five color indicator cards of yellow, orange, red, deep red and purple, the respiration of the compost or soil can be quickly identified, and it can also indicate the five levels of microbial activity or maturity stage. The test paper of the present invention has good sensitivity and high accuracy, and the color change range of the high-range test paper matches 1-50 mg of CO 2 Respiration rate, low range test strip color change range to match 0.1‑5mg of CO 2 Respiration rate; the method of use is simple and fast, and the sample test can be completed within 30 minutes without the need for expensive testing instruments.

Description

technical field [0001] The invention belongs to the field of environmental chemistry and microbiology, and relates to a gel test paper for rapid detection of microbial respiration rate, in particular to a rapid detection of microbial CO in compost and soil. 2 Gel test strip for respiration rate and method of use thereof. Background technique [0002] CO 2 Respiration is common in aerobic microorganisms and is the process by which microorganisms consume O 2 It is the final product of complete oxidative metabolism with organic matter. Soil and compost are common microbial parasitic environments in environmental media. After a large number of aerobic microorganisms utilize and degrade organic matter, they release CO under aerobic conditions. 2 . So CO 2 Respiration rate is an important indicator of soil and compost microbial activity, and is of great significance for indicating soil microbial activity and compost maturity. Traditional CO 2 Respiratory testing, often usin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/24
CPCG01N33/24G01N33/243
Inventor 徐卫平惠雪松许建强包永明关水周豪王占军姜慧敏王文霞刘玉婷
Owner DALIAN UNIV OF TECH
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