Device and method for detecting total number of colonies in foods

Abstract

The invention relates to the technical field of food microorganism detection and discloses a device and method for detecting the total number of colonies in foods. The device comprises a reaction chamber, an electric signal amplifier, an electric signal data acquisition card and an electric signal processor. A proton exchange membrane is arranged in the reaction chamber. The reaction chamber is divided into an anode reaction chamber and a cathode reaction chamber through the proton exchange membrane. A reaction system and electrodes are arranged in each one of the anode reaction chamber and the cathode reaction chamber. The electric signal amplifier is respectively connected to the electrodes in the anode reaction chamber and the cathode reaction chamber. The electric signal data acquisition card is respectively connected to the electric signal amplifier and the electric signal processor. The device and method utilize the principle of electrochemistry and utilizes electrons produced bymicrobial respiration as detection objects. Through the correlation curve of voltage values and the total number of colonies, which is drawn according to the known concentration of microbial broth, the total number of colonies in the sample are obtained in 30min and accuracy is 95% or more.

Description

technical field [0001] The invention relates to the technical field of food microorganism detection, in particular to a detection device and a detection method for detecting the total number of bacterial colonies in food. Background technique [0002] Microbial contamination of food is the primary problem affecting food safety. The new food safety law clearly stipulates the inspection content of food before leaving the factory. Among them, microorganisms, especially the total number of colonies, are mandatory inspection items before food leaves the factory. At present, the detection of the total number of colonies in food still adopts the plate counting method, which has a series of problems such as long inspection cycle (generally takes 2-3 days), complicated operation, and high detection cost. Especially for products with a shelf life of only 5-7 days (such as soybean products, meat products, etc.), the products can only leave the factory after passing the microbial test. ...

AI Technical Summary

Problems solved by technology

At present, common rapid detection methods for the total number of colonies in food include ready-to-use paper method, electrical impedance method, micro-colony count method, ATP bioluminescence method, and near-infrared spectroscopy, among which ready-to-use paper method, electrical impedance method, Although the micro-colony counting method has the characteristics of high accuracy and high sensitivity, the detection time needs at least 7-10 hours because the microorganisms need to be cultivated and reproduced during the detection; Due to different detection principles, the detection time can be shortened to 10 minutes or even a few seconds, but the ATP bioluminescence method is easily interfered by impurities, etc., and the near-infrared spectroscopy method has the problem of insufficient model stability.

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