186 results about "Lactoglobulins" patented technology

PCT No. PCT / SE97 / 00237 Sec. 371 Date Dec. 29, 1998 Sec. 102(e) Date Dec. 29, 1998 PCT Filed Feb. 14, 1997 PCT Pub. No. WO97 / 29825 PCT Pub. Date Aug. 21, 1997Process for separating off a peptide or a nucleic acid by an anion exchanger (I) characterized in that a) the anion exchanger (I) exhibits ligands, which (i) contain a primary, secondary or tertiary amino group and (ii) are covalently bound to an organic polymer (matrix), b) there on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen in the ligands is a hydroxyl group or a primary, secondary or tertiary amino group, and c) the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, beta -lactoglobulin 1 and beta -lactoglobulin 2 on the anion exchanger is higher than the elution ionic strength required for a quaternary comparative ion exchanger.

The present invention is directed to co-assembled nanoparticle composition comprising denatured β-lactoglobulin and at least one nutraceutical compound, specifically polyphenols, such as EGCG, compositions comprising same and methods of preparing thereof.

The invention discloses a preparation method for a biological agent for preventing and controlling human papilloma virus infection. The preparation method includes following steps: dissolving 3-hydroxy-phthalic anhydride HP into dimethyl sulfoxide DMSO to obtain saturated HP solution; dissolving beta-lactoglobulin beta-LG into 0.1M of sodium phosphate solution with pH (potential of hydrogen) of 8.5 to obtain protein solution with protein final concentration of 20mg / mL; and equally dividing the HP solution into five parts, adding the HP solution into the protein solution at every 12 minutes, shaking and mixing, adjusting pH of IMNaOH to be 8.5, leading the final concentration of anhydride in a reaction system to be 60mM, standing for 1 hour at the temperature of 25 DEG C, dialyzing by PBS (phosphate buffer solution) with pH of 7.4, performing filtration sterilization by a 0.45uM microfiltration membrane, and storing at the temperature of 4 DEG C so as to obtain a finished product. The biological agent has the advantages of capabilities of stopping HPV (human papilloma virus) from invading cells and stopping virus infection from spreading, safety, stability, low cost and the like.

A process for modifying the lactoprotein by enzyme method features that the immobilized composite enzyme, the ionically regulatory protein structure and heat treating technique are used as selectively hydrolyze the alpha S-casein and beta-lactoglobulin in lactoprotein, resulting in easy digestion and low sensitization. It can be used for composite milk for baby, old man and patient.

The invention discloses a method for separating and detecting whey proteins, which comprises the following steps: pretreating the whey protein; and completely separating bovine alpha-lactalbumin, bovine beta a-lactoglobulin, and bovine beta b-lactoglobulin in a sample by an ultra-performance liquid chromatography column taking 1.7 mu m ethylidene bridged hybrid (BEH) particles as a filler, and performing section scan by a mass analyzer to quantitate the unmodified bovine lactalbumin and lactoglobulin. In the method, human alpha-lactalbumin is added as internal standard. The method can accurately measure the contents of the three types of unmodified bovine whey proteins in multiple kinds of food, and has simple pretreatment, high sensitivity, quick detection speed and good selectivity.

The invention discloses a method for determining the content of beta-lactoglobulin in milk powder. The method comprises the following steps: (1) selecting TK-11 as a specific peptide fragment and conducting beta-lactoglobulin quantification; (2) synthesizing the peptide fragment, and conducting isotope labeling on the peptide fragment; (3) accurately quantifying the synthesized standard peptide fragment by adopting an isotope dilution mass spectrography; (4) conducting sample treatment and enzyme digestion; (5) hygroplasm analysis, namely filtering the solution after enzyme digestion through a filter film, and then carrying out selective ion scanning analysis on the filtrate by adopting mass spectrography; and (6) calculating according to a formula, so as to complete the determination on the content of beta-lactoglobulin in milk powder. By adopting the method, determination results are accurate and reasonable.

Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.

The present invention provides an immunological detection method that can detect milk allergens, allergens of albumen, flour, buckwheat and peanut with high sensitivity in foods containing these allergens regardless they are denatured / native, and a detection kit to be used therefor. It is a method for detecting allergens by using 2 or more monoclonal antibodies recognizing native and denatured milk allergens, native and denatured albumen allergens, native and denatured flour allergens, native and denatured buckwheat allergens, and native and denatured peanut allergens, using asl casein which is the main protein of milk casein, β-lactoglobulin which is the main protein of whey, ovalubumin and ovomucoid which are main proteins of albumen, gliadin which is the main protein of flour, protein with a molecular weight of 24 kDa and 76 kDa which are main proteins of buckwheat, and Ara h1 which is the main protein of peanut as an index.

The invention provides an active lactoprotein extracted from yak milk and its extraction method and use, wherein the active constituents by weight include beta-lactoglobulin 51-54, alpha-lactoglobulin 20-23, albumin bovine 9.0-11, lactoferrin 1.0-2.0, immunoglobulin 11-14, other trace nourishing elements 1.0-2.0, the preparation comprises the steps of choosing raw material, removing impurities and degreasing through centrifugation, low-temperature sterilizing, condensating the milk, degerming and thickening, thin film evaporating, freeze drying and asepsis vacuum packaging. The extracted active milk protein has substantial function for enhancing cell vitality and body defensiveness.

The invention provides a method for decreasing beta-lactoglobulin in concentrated whey albumen powder through biotic compound protein enzymolysis. The method comprises the following steps of: (1) dissolving, heating and preserving heat: a certain amount of concentrated whey albumen powder is dissolved in water, the mass ratio of concentrated whey albumen powder is 1.6-5.0 percent, the solution is subjected to heat preservation at the temperature of 50-100 DEG C for 20-60min, and the solution is cooled to 45 DEG C; (2) carrying out enzymolysis: compound enzyme is added into whey albumen liquid, the mass ratio of the concentrated whey albumen powder to the compound enzyme is (40-80):1, and enzymolysis is carried out at the temperature of 30-45 DEG C for 3-8 hours; (3) evaporating and concentrating: a sterilization treatment is processed before concentration, the temperature of sterilization is controlled to be 100-125 DEG C, the time for heat preservation is 90-180s, the temperature of concentration is controlled to be 50-85 DEG C, and the concentration of solid materials after concentration is 25-35 percent; and (4) atomizing and drying: the air inlet temperature of hot wind is 200-210 DEG C, the air outlet temperature of hot wind is 80-90 DEG C, and the material liquid temperature is 65-75 DEG C. The method has the advantages that 1, high quality ingredient of lactoglobulin and the like in cow milk is persisted, and the category and the proportion of amino acid are also persisted; and 2, a part of peptide is generated through hydrolysis (enzymolysis), thus the phenomenon that an infant is allergic to lactoprotein is more facilitated to be reduced.

An ultrasound-assisted simulated digestion method of a milk protein active peptide and an application thereof in health foods, pertaining to the technical field of intensive processing of dairy products and preparation of health foods. The method firstly employs ultrasonic pretreatment of casein and β-lactoglobulin, followed by enzymatic hydrolysis with a protease to prepare casein and β-lactoglobulin polypeptide, and traces the activity of the polypeptide by simulating gastrointestinal digestion, and then simulates absorption by intestinal epithelial cells with Caco-2 cells, to characterize a highly active milk protein polypeptide digested by the gastrointestinal tract and absorbed by the Caco-2 cells simulating absorption by the inner wall of the small intestine. The method has identified five such highly active milk protein polypeptides.

The invention discloses a preparation method, a product and application of lactobacillus whole peptidoglycan with an effect for resisting beta-lactoglobulin hypersusceptibility and relates to a microemulsion preparation prepared from the lactobacillus whole peptidoglycan obtained in the invention. The microemulsion preparation is prepared by the following steps of: with the lactobacillus whole peptidoglycan as a main effective component, heating the whole peptidoglycan, an oil phase, a surfactant, a cosurfactant and water at 70DEG C by stirring to enable the whole peptidoglycan to be completely dissolved; and uniformly mixing to obtain a semitransparent emulsion product with the liquid drop diameter of 30-45nm. After the lactobacillus whole peptidoglycan microemulsion preparation is orally taken to enter the interior of the body, lymphocyte can be effectively stimulated to secrete IL-12 and IFN-gamma; the IL-4 level is reduced; excessive hyperfunction of Th2 cells is reversed; and the lactobacillus whole peptidoglycan microemulsion preparation plays an important role of inhibiting beta-lactoglobulin allergic inflammation mediated by IgE and has potential application values for developing an immunopotentiator with antiallergic performance and the like.

A long-acting improver of muscular fatigue characterized by comprising 4 kinds of amino acids made up of leucine, isoleucine, valine and glutamine, and a whey protein component (whey protein and / or decomposition product of whey protein). At least one of a whey protein isolate (WPI), a whey protein concentrate (WPC), β-lactoglobulin, and α-lactalbumin is used as the whey protein. Novel food or drink, and pharmaceuticals which exhibit sustained recovery effects on muscular fatigue are provided.

The invention discloses a buffalo milk active-bacteria whey beverage and a preparation method thereof. The whey beverage is obtained through the steps of: mixing byproduct whey generated in the preparation process of buffalo milk cheese with fermented buffalo sour milk in a certain ratio, appropriately adding sugar, a stabilizer and spice to obtain a mixture, then concocting, homogenizing and filling the mixture, so as to obtain the buffalo milk active-bacteria whey beverage. The whey beverage has the complete nutritional ingredients of the whey and the sour milk, contains protein, various amino acids, vitamins and mineral substances needed by human bodies, and especially contains various active proteins such as alpha-lactalbumin, beta-lactoglobulin, immune globulin, lactoferrin, lactoperoxidase and active lactobacillus which are contained in the whey; and the beverage is unique in flavor, exquisite in mouth feel, rich in nutrition, and capable of improving the gastrointestinal function and enhancing the immunity.

The invention discloses an immune-colloidal gold test strip for detecting adulterant cow milk in camel milk and application thereof. A camel milk alpha-lactalbumin gold-labeled antibody, a camel milkalphas1-casein gold-labeled antibody, a cow milk beta-lactoglobulin gold-labeled antibody and a cow milk k-casein gold-labeled antibody are prepared; a test strip I for detecting a whey protein component and a test strip II for detecting a casein component are prepared respectively; and then an immune-colloidal gold test strip for detecting adulterant cow milk in camel milk is obtained. The test strip is capable of monitoring the cow source and camel source protein of the milk and the dairy product simultaneously and has the high practicability and the great development value in the adulteration monitoring field of the camel milk.

The invention discloses an aptamer probe for detecting alpha-lactalbumin, beta-lactoglobulin and lactoferrin, and belongs to the technical field of IA (immunoassay) of food analysis. The nucleotide sequence of the aptamer probe for detecting alpha-lactalbumin, beta-lactoglobulin and lactoferrin disclosed by the invention is shown as SEQ ID NO. 1-3. According to a method disclosed by the invention,the contents of the alpha-lactalbumin, the beta-lactoglobulin and the lactoferrin can be simultaneously detected quickly and accurately; the method is high in sensitivity, short in detection time andsimple and easy to operate.

The present invention relates to the field of microbial technology, more specifically, the invention relates to a Lactobacillus casei which can ferment to reduce the antigen capability of the beta-lactoglobulin and its culture method and uses.

Our objective is to provide a novel lipid metabolism-improving reagent effective in preventing and treating hyperlipidemia and obesity and to provide this reagent in food, beverages, nutritional supplements, and fodders with the ability to improve lipid metabolism. The whey protein hydrolysate as the active ingredient in the lipid metabolism-improving reagent has a molecular weight distribution of 10 kDa or less, with the main peak between 200 Da to 3 kDa, average peptide chain length (APL) of 2-8, free amino acid content of 20% or less, antigenicity of 1 / 10,000 or less compared with that of β-lactoglobulin.

This invention discloses a method for constructing animal mammary gland-specific vector with high expression level. The method comprises: (1) utilizing partial non-coding sequence (about 500bp) of cytomegalovirus as an enhancer; (2) inserting the enhancer between a lactoprotein regulatory sequence and a functional gene (Cdna or genome DNA) to construct a mammary gland-specific expression vector. The regulatory sequence is non-coding sequence of casein or lactoglobulin. The specific expression vector can increase the expression level and the expression efficiency of a transgenic animal mammary gland bioreactor.

Provided herein are compositions and methods for producing recombinant milk proteins, as well as food compositions comprising the same. In aspects, the recombinant milk proteins of the disclosure are recombinant fusion proteins comprising casein and beta-lactoglobulin.

The present invention provides a composition for treating and / or preventing influenza, a method and an application thereof. The composition contains protein treated with acid anhydride and another drug for treating and / or preventing influenza. The acid anhydride-treated protein prepared by the invention is acid anhydride-treated lactoglobulin. An in vitro experiment shows that the protein can broadly inhibit influenza virus infected cells of various subtypes. In animal in vivo experiments, the protein has a significant prevention and treatment effect on influenza virus infection. Influenza viruses have many subtypes and high mutation rates, so that the development of broad-spectrum anti-influenza preparations is very important. The acid anhydride-treated protein can not only inhibits the infection of many seasonal influenza viruses, but also significantly inhibits the highly pathogenic avian influenza virus H7N9, and the influenza virus H1N1 (2009) which caused a pandemic, suggesting that the protein can be developed as a highly effective and broad-spectrum biological preparation for the prevention and treatment of influenza viruses.

The invention relates to an ordered double mesoporous silicon oxide material, which belongs to the technical field of material preparation. The present invention utilizes tetraethyl orthosilicate and 1-hexadecyl-3-methylimidazole chloride in an acidic aqueous solution of monodisperse polymethyl methacrylate microcolloid emulsion balls (ball diameter 63±3nm) Self-assembly, through rapid decompression suction filtration, roasting to remove the organic template, to obtain a double mesopore with a pore diameter of 38±2nm and a hexagonal phase small mesopore with a pore wall of 2.7±2nm, which are arranged in an orderly three-dimensional direction Porous silica material. The ordered double mesoporous structure was confirmed by XRD, SEM, TEM and N2 adsorption experiments, and it is a new type of ordered double mesoporous silica material. The material has high adsorption performance and special pore size distribution range, and can be used to prepare catalysts and adsorbents. The invention provides a matrix structure for the research and development of new catalysts, and is beneficial to the further development of multifunctional new catalysts.

The invention discloses lactobacillus rhamnosus and an application thereof. The lactobacillus rhamnosus is named as lactobacillus rhamnosus AU9260 in taxonomy, and the preservation number of the lactobacillus rhamnosus is CGMCC No.21662. The strain has excellent in-vitro probiotic characteristics such as simulated gastrointestinal fluid tolerance, bacteriostatic activity, adhesion to Caco-2 cells and the like, and is preliminarily judged to be safe. In addition, the strain has a potential function of relieving and / or preventing allergic diseases of hosts, can significantly reduce histamine, specific IgE and cell factors in serum of mice induced and sensitized by beta-lactoglobulin, and can also protect damage to intestinal mucosa structures of the mice caused by allergy and regulate intestinal flora structures. The lactobacillus rhamnosus can be used as foods and / or health-care foods with a desensitization effect.

The invention relates to a refreshing and brain-strengthening lozenge which is characterized in that the troche comprises the following main ingredients by weight: 300-400kg of oat, 90-130kg of milk powder, 10-20kg of whey protein powder, 90-130kg of white sugar, 70-90kg of peanuts, 50-70kg of walnuts, 50-70kg of soybeans, 50-70kg of bananas, 50-70kg of lettuces, 40-60kg of sesames, 30-50kg of longan pulps, 30-50kg of agarics, 20-30kg of mints, 20-30kg of lemons and 2-4kg of lucid ganoderma. The lozenge provided by the invention is rich in proteins, mineral substances as well as vitamins A and E, cellulose, lecithin, beta-lactoglobulin, alpha-lactoglobulin, immune globulins and lactoferrin, is beneficial for nutritional supplement of brain, and can eliminate fatigue and has the effects of refreshing and strengthening brain, relieving the pressure and strengthening brain and reinforcing intelligence, so that people are full of energy and stay focused.

The present invention provides an immunological detection method that can detect milk allergens, allergens of albumen, flour, buckwheat and peanut with high sensitivity in foods containing these allergens regardless they are denatured / native, and a detection kit to be used therefor. It is a method for detecting allergens by using 2 or more monoclonal antibodies recognizing native and denatured milk allergens, native and denatured albumen allergens, native and denatured flour allergens, native and denatured buckwheat allergens, and native and denatured peanut allergens, using asl casein which is the main protein of milk casein, β-lactoglobulin which is the main protein of whey, ovalubumin and ovomucoid which are main proteins of albumen, gliadin which is the main protein of flour, protein with a molecular weight of 24 kDa and 76 kDa which are main proteins of buckwheat, and Ara h1 which is the main protein of peanut as an index.

The invention provides a method for knocking out a cattle beta-lactoglobulin gene by using zinc finger nucleases (ZFNs), comprising the following steps of: according to a cattle beta-lactoglobulin gene sequence, designing ZFNs specific site expression vector and transplanting the ZFNs specific site expression vector into cattle fibroblast; and obtaining cells with the beta-lactoglobulin gene knocked out. By using ZFNs mediated gene knockout, one-time transfection can be realized so as to obtain cell clones with biallelic genes knocked out, which is difficultly achieved in the conventional gene targeting process. The drug screening process is saved. The method disclosed by the invention is advantageous for forming monoclonal cells. The process required by cells for resisting drug toxic process is avoided. The method plays a key role in the improvement of subsequent somatic cell nuclear transplantation efficiency and embryonic development quality. Simultaneously, resistance genes are not contained; and the biological safety evaluation process is greatly simplified.

This invention relates to compositions for delaying aging. The compositions comprise branched-chain amino acids and whey protein, and combinations thereof, containing the same, such as L-leucine, L-isoleucine, L-valine, lactoferrin, and β-lactoglobulin. The compositions are heat stable when dissolved in water at near neutral pH. The compositions are palatable and suitable for delivering orally administrable anti-aging agents such as ω-3 fatty acids, coenzyme Q10, xanthophylls, L-arginine, and L-glutathione.

Provided herein are compositions and methods for producing recombinant milk proteins, as well as food compositions comprising the same. In aspects, the recombinant milk proteins of the disclosure are recombinant fusion proteins comprising casein and beta-lactoglobulin.