Method for constructing idiosyncratic carrier of galactophore of transgenic animal in high expression level

Abstract

This invention discloses a method for constructing animal mammary gland-specific vector with high expression level. The method comprises: (1) utilizing partial non-coding sequence (about 500bp) of cytomegalovirus as an enhancer; (2) inserting the enhancer between a lactoprotein regulatory sequence and a functional gene (Cdna or genome DNA) to construct a mammary gland-specific expression vector. The regulatory sequence is non-coding sequence of casein or lactoglobulin. The specific expression vector can increase the expression level and the expression efficiency of a transgenic animal mammary gland bioreactor.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and the technology is applied to prepare transgenic animal mammary gland bioreactors. Background technique [0002] Transgenic animal mammary gland bioreactor is to use animal mammary gland organs to produce biological drugs or biological products, which has high commercial application value, but the efficiency in the preparation of mammary gland bioreactor is low, and the expression rate and expression level in transgenic animal mammary gland are Random and indeterminate, most were expressed at low levels. At present, the general mammary gland-specific expression gene is composed of milk protein regulatory element and functional gene. Although this gene component has a regulatory effect on the initiation and specificity of functional gene expression, its expression level and expression rate are very low. Its overall gene structure is: 5'-terminal milk protein regulatory sequence→function...

AI Technical Summary

Problems solved by technology

[0003] At present, the main problem to be solved for animal mammary gland-specific expression is still the expression level, and it is difficult to achieve a high level by simply using milk protein regulatory sequences in the building block