48 results about "Heimia salicifolia" patented technology

Disclosed is a method for making a bioreactor comprising a foreign target gene, special selectable markers and Dunaliella Salina as host. It is prepared by the genetic transformation techniques that include introducing a foreign target gene into the cells of Dunaliella Salina and screening the transformed cells of Dunaliella Salina. The bioreactor of the present invention can be used as a safe and cheap production system for proteins of pharmaceutical interest including vaccines, especially oral products, in a large scale, because the cells of Dunaliella Salina are easy of genetic manipulation in preparation of the bioreactor, nontoxic and edible for humans and animals, and harmless to the environment.

The invention discloses a health care product containing Noni juice. The health care product is prepared from the following raw materials in parts by weight: 1-10 parts of Noni juice, 0.1-2 parts of dunaliella salina, 1-10 parts of poria cocos, 1-8 parts of coix seed, 1-6 parts of semen cassiae, 1-6 parts of chrysanthemum, 1-6 parts of fructus amomi (which is added or not added), 1-5 parts of mesona blume, 1-8 parts of barbary wolfberry fruit, 1-6 parts of gynura procumbens, 0.1-2 parts of tissue culture of saussurea involucrata, 1-5 parts of inulin, 1-6 parts of honeysuckle, 1-4 parts of hovenia dulcis thumb, 1-6 parts of cape jasmine fruit, 1-6 parts of folium mori, 1-5 parts of lophatherum gracile, 1-8 parts of the root of kudzu vine, 1-6 parts of dandelion, 1-4 parts of mint, 1-6 parts of fingered citron, 1-5 parts of citron, 1-6 parts of gorgon fruit, 1-5 parts of Chinese mosla herb, 1-5 parts of vermilion, 1-5 parts of lotus leaves, 1-8 parts of rhizoma polygonatum, 1-7 parts of wild jujube seeds, 1-7 parts of raspberries, 1-4 parts of male flowers of eucommia ulmoides oliv and 0.1-2 parts of cordyceps militaris. The health care product is skillfully collocated in the five aspects of the heart, liver, spleen, lung and kidneys according to the principle of treating hot and humid stagnation and applying on sanjiao (triple energizers), so that the body is comprehensively and harmoniously conditioned, and diseases can be controlled; after the health care product is taken for a long time, the damp and hot physique can be conditioned, and the body is effectively promoted to reach a state of balance of yin and yang.

The invention provides a preparation method of transgenic dunaliella salina for improving the photosynthesis efficiency, which is characterized by comprising the following steps: colonizing or reforming genes related to carbon fixation and photosynthesis in a photosynthetic autotroph; transferring the genes into a chloroplast genome or nuclear genome of the dunaliella salina for expressing; successively and selectively culturing; and finally obtaining a stable transgenic dunaliella salina strain. By introducing independent or combined expression of high-efficiency genes related to carbon fixation and photosynthesis, the invention improves the concentration and the transferring speed of CO2 in the transgenic dunaliella salina and ensures that the capability of the transgenic dunaliella salina using the CO2 for photosynthesis can be improved, thereby promoting the autotrophic growth of the transgenic dunaliella salina, accelerating and increasing the accumulation of biomass liveweight of dunaliella salina cells and meeting the requirement on scale production of biodiesel and metabolin thereof as well as genetic engineering products.

A method for artificial oxytocin and fertilization of Mytilus coruscus comprises the following steps of a parent cultivating pool: building an outdoor cultivating cement pool with the dimension of 10cmx1.5mx1m, stocking inflatable stones, covering canvas above the cement pool to prevent rain and sunlight, and shading the periphery of the cement pool by dark cloth to regulate strength of light; selecting Mytilus coruscus which has new and integral shell surfaces, full gonads, complete gill, light red ovaries and white testes, and is free of damage and powerful in closure of double shells; preparing baits, wherein chlamydomonas live baits including cultured Dunaliella viridis, latymonas subcordiformis and Chaetoceros serve as primary baits, and artificial compound feed including spirulina powder, chlorella powder and yeast tablets serves as secondary baits; drying parent Mytilus coruscus with developed gonads in the shade for 6-8 hours, stimulating the parent parent Mytilus coruscus for 1 hour through running water, and feeding the parent parent Mytilus coruscus into prepared warming seawater.

The invention discloses a maca dunaliella salina compound preparation. The maca dunaliella salina compound preparation comprises dunaliella salina and spirulina and is prepared from the following materials: 3-10 percent of dunaliella salina, 40-45 percent of spirulina and 45-55 percent of maca. The maca dunaliella salina compound preparation has the effects of building body and tonifying Yang, curing organs, maintaining beauty and keeping young and improving the digestive system, achieves a two-way blood-pressure regulating function, as well as a two-way blood sugar regulating and balancing function, can regulate body metabolism and resist aging, tumor and fatigue and enhance the immunity, and has remarkable effects on cardio-cerebrovascular diseases, chronic diseases, senile dementia, climacteric syndrome and the like. When drunk frequently, the maca dunaliella salina compound preparation increases sexual intercourse, keep youth for a long time and prolongs the lifetime, has the advantages of relaxing tendons and activating collaterals, activating blood circulation to dissipate blood stasis, dispelling wind to eliminate dampness and strengthening bones and tendons and can remarkably improve memory.

The invention belongs to the technical field of biological culture of algae, and relates to a dunaliella culture method. The dunaliella culture method comprises the following steps: at first, regulating the light intensity and temperature in a dunaliella culture reactor to adapt to dunaliella propagation, inoculating dunaliella into a dunaliella culture agent containing a salt, a nitrogen source (KNO3), a phosphorus source (KH2PO4) and an inorganic carbon source (NaHCO4), respectively measuring the nitrogen source, the phosphorus source and the inorganic carbon source in the dunaliella culture agent after a growth period, a logarithm growth period and a platform period of dunaliella culture, making the nitrogen source, the phosphorus source and the inorganic carbon source respectively reach set values, and measuring dunaliella biomass to complete the dunaliella propagation; regulating the light intensity and the temperature to adapt to beta-carotene accumulation, respectively adding table salt, KNO3, KH2PO4 and NaHCO4 into the dunaliella culture agent to set values, culturing until the dunaliella becomes brown, and then the beta-carotene is accumulated and the dunaliella culture is completed. The dunaliella culture method is scientific and reasonable in culture process, simple in steps, stable in performance, free of external environment influence and high in product utilization rate.

The invention provides a method for preparing hydrolyzed oligosaccharides and short peptides from salt algae residues, which belongs to the technical field of salt algae utilization. The method adopts beta-carotene removed salt algae residues as raw materials, separation and enzymatic hydrolysis are repeatedly performed, and then various products are made after concentration and drying. The method provided by the invention comprehensively utilizes the salt algae residues to make various daily use chemicals, medicines and health products, such as skin energy activating solution, hydrolyzed salt algae oligosaccharides and polysaccharide and short peptides. The residue and precipitate of the hydrolyzed salt algae is retained, but not discarded during production process, and then is used as fermentation base material for microbial fertilizer preparation. The products prepared through the method can be wildly applied to products for skin care, moisture preservation, nutrition, antioxidation and anti-ageing, can be applied to health products and anti-tumor products, and also can be served as single cell protein products to complement nitrogen nutrition and radioprotective products.

The present invention relates to saline algae, provides one kind of low temperature tolerant saline algae and its ultraviolet mutagenesis breeding process and identifying method. The low temperature tolerant saline algae features its light illuminating growth at 3-10deg.c to reach (1.05-8.11)x10<6> cell / ml in the cell density 2.1-21 times higher than the contrast; the genetic similarity coefficient to wild strain of 0.680-0.910; and increased or decreased protein zone(s) compared with wild strain.

The invention discloses a culturing method of salt algae, which is characterized by the following: seeding bacillus in the culture medium; obtaining the growing speed at 4.135*105 / d and biological quantity at 846.88mg .L-1 and accumulating quantity of beta-carotene at 125.4mg .L-1; accelerating the development of salt algae and beta-carotene industry.

The present invention relates to a kind of saline alga photosynthetic metabolic pathway key enzyme NADP-glyceraldehyde-3-phosphate dehydrogenase gene, coded protein and its clone and protein expression method. The invented saline alga photosynthetic metabolic pathway key enzyme NADP-glyceraldehyde-3-phosphate dehydrogenase gene has the base sequence showed by SEQ NO 5, and its coded protein has the amino acid sequence showed by SEQ NO 6. Said invention uses homologous fragment of glyceraldehydes-3-phosphate dehydrogenase gene originated from chlamydomonas as probe and firstly clones a glyceraldehydes-3 phosphate dehydrogenase specialized by saline alga. Besides, said invention makes primary analysis for its sequence and coded protein sequence, at the same time makes primary function analysis for said gene.

The invention relates to a culture method of Dunaliella and application of Dunaliella in biomass energy, which relate to the Dunaliella. The invention provides a culture method of Dunaliella which is simple and economical, not only can be used as the biomass energy, but also can efficiently capture and store carbon dioxide, and the application of the Dunaliella in the biomass energy. The culture method is characterized by filtering in sea water; inoculating the dunaliella seed in sea water culture base, culturing the seed for 14 to 35 days, mixing continuously every day, culturing the dunaliella for a year, and harvesting the dunaliella cell when density of the dunaliella reaches 104 to 106 per ml. The dunaliella is used as a material of the biomass energy, high-concentration sea water isused as culture base, and the carbon dioxide is assimilated through the growth of the dunaliella so as to accumulate the biomass energy materials such as grease, starch and the like.

The invention provides a dunaliella salina cDNA library induced by high salt as well as screening its cDNA fragment and gene. Wherein, it constructs the library with inhibiting hybridization, and discloses the cDNA fragment and dunaliella salina Na+ / H2PO4- co-ttransfer membrane channel protein cDNA, whole gene and its amino acid sequence. This cDNA can screen the specific express of dunaliella salina induced by high salt, and settles foundation for corresponding molecular mechanism research.

The invention relates to a natural dunaliella salina beta-carotene vitamin E soft capsule. The soft capsule comprises a capsule liquid and a casing, wherein the capsule liquid contains the following components in parts by mass: 2 to 7 parts of dunaliella salina beta-carotene, 7 to 14 parts of vitamin E and 279 to 291 parts of vegetable oil, wherein the dunaliella salina beta-carotene is natural beta-carotene which is extracted from dunaliella salina via a vegetable oil extraction method, an alkaline extraction method, an enzymolysis method or a supercritical extraction method. The soft capsule has a function of enhancing immunity and can perform auxiliary protection on radiation hazards. The soft capsule is mature in process, stable in quality, high in production efficiency and easily suitable for market promotion.

The invention relates to facial masks, and discloses a Dunaliella salina natural active ingredient facial mask and a preparation method thereof in order to avoid phenomena of stains, wrinkles and the like brought by cell oxidization. The following technical scheme provided by the invention is that a formula of the Dunaliella salina natural active ingredient facial mask is characterized in that when the Dunaliella salina natural active ingredient facial mask is in a liquid state, the Dunaliella salina natural active ingredient facial mask is prepared from the following ingredients in percentage by weight: 0.3 to 4 percent of sodium lactate, 0.3 to 1 percent of acrylate / C10 to 30 alkanol acrylate cross-linked polymer kali salt, 0.3 to 1.5 percent of trehalose, 0.02 to 0.2 percent of sodium hyaluronate, 0.1 to 0.5 percent of phenoxyethanol, 0.01 to 0.03 percent of EDTA (Ethylene Diamine Tetraacetic Acid) disodium salt, 10 to 20 percent of functional grease extract of Dunaliella salina natural active ingredients and 65 to 80 percent of deionized water. The Dunaliella salina natural active ingredient facial mask disclosed by the invention has a stronger antioxidant effect, a better moisturizing effect and a more remarkable whitening effect and also has a function of relieving skin aging, and the phenomena of the stains, the wrinkles and the like brought by cell oxidization are avoided.

The invention discloses a method for extracting chlorophyll from dunaliella by using inorganic alkali, which comprises the following steps: (1) cooling and freezing dunaliella mud or dunaliella powder added with a proper amount of water, unfreezing in a water bath till ice crystals are eliminated; (2) regulating pH value by using aqueous solution of an inorganic alkali, and extracting for 3 to 5 hours at 60 to 70 DEG C; (3) centrifuging the extract solution and collecting supernate; (4) regulating the pH value of the supernate by using an inorganic acid to precipitate chlorophyll; and (5) centrifuging, removing supernate to obtain chlorophyll and freezing and drying the chlorophyll. The method disclosed by the invention overcomes the drawback of organic solvent residue of the product obtained by an organic solvent extraction method and the drawbacks of big investment and high economic cost of a supercritical extraction. When the method is used, a convenience is provided for chlorophyll extraction and the trouble brought by the need of removing acetone, petroleum ether and other organic solvents in a subsequent polysaccharide extraction process is avoided.

A transferred hepatitis B surface antigen (HBsAg) gene halophila is prepared by recombining the exogenous gene HBsAg into the cells of eucaryotic monocell halophila, so obtaining the transgenic halophila used to prepare the oral vaccine is hepatitis B. Its advantages are high reproduction speed, simple culture condition, low cost, and rich nutrients contained by halophila cells.

The invention discloses a dunaliella salina culture medium. The dunaliella salina culture medium is prepared from 5-10 g / L of NaCl, 0.5 g / L of NaNO3, 45-100 mg / L of KH2PO4, 1 mg / L of ferric citrate, 0.2-0.3 g / L of potassium acetate, 0.25-0.4 g / L of glycine, 1-10 mg / L of dichlorophenoxy triethyl-amine DCPTA, 23 mu g / L of ZnSO4.4H2O, 178 mu g / L of MnCl2.4H2O, 10 mu g / L of CuSO4.5H2O, 7.3 mu g / L of Na2MoO4.2H2O, and 12 mu g / L of CoCl2.6H2O. The culture medium prepared according to the invention has the characteristics of high growth speed, large cell density, and the like in the process of culturing dunaliella salina.

The invention discloses a preparation technology for wine with arctium lappa and dunaliella salina, and belongs to the field of health-care wine. The traditional seaweed wine is mainly prepared by a method of directly soaking seaweed into food grain wine or edible wine, not only the color is turbid, the mouth feel is poor, the technology is crude, the cost is higher, the nutrient components are monotonous, but also the extraction rates of the nutrient components, such as carotene, glycerine, EPA, DHA, chlorophyll a, gamma-linolenic acids, macrolide, terpenoid, xanthophyll and sterol, in the seaweed are low. The preparation technology disclosed by the invention comprises the following steps: using food grain alcohol and distilled water for extracting dunaliella salina, sufficiently extracting the chlorophyll a, beta-carotene, phycocyanin, micro algae polysaccharide and CGF active factors in the dunaliella salina, and remaining the components of inulin, vitamins, protein and the like in the arctium lappa. The wine has the advantages that the product color is clear, and the taste of the wine is mellow, sweet, refreshing, fragrant and tangy; the wine can promote blood circulation for removing blood stasis, replenish essence for consolidating body resistance, can nourish the heart, sooth the nerves, dispel wind, eliminate dampness, maintain beauty and prolong the life; the operation is simple and convenient, the technological process is short, the raw materials are abundant, the benefits are high, and the market prospect is broad.

The invention relates to a harvesting method of Dunaliella salina. The harvesting method comprises a first part of determining the pH (potential of Hydrogen) value of a Dunaliella salina algal solution; a second part of calculating the amount of sodium hydroxide used for regulating the pH value of a certain volume of the Dunaliella salina algal solution to be 12 to flocculate the Dunaliella salina in the algal solution; a third part of determining time needed by complete flocculation; a fourth part of collecting the Dunaliella salina; a fifth part of estimating a harvesting rate. In comparison with a centrifugal method in the prior art, by using the harvesting method of the Dunaliella salina, the treatment efficiency is obviously improved; the harvesting efficiency is drastically improved; the yield of algal powder is obviously promoted; meanwhile, the injuries to algal cells of the Dunaliella salina are reduced; the consumption of electric energy is decreased.

The invention discloses a culture method for improving the contents and the bioavailability of mineral elements in dunaliella salina cells. The culture method is mainly characterized in that ion concentrations of specific inorganic mineral elements of calcium, magnesium, iron, zinc and selenium are selectively added in an existing dunaliella salina culture medium, so that the contents of specific biological mineral elements in the dunaliella salina cells can be increased and compositions of different mineral elements can be adjusted; meanwhile, by transforming inorganic mineral nutrition into biological minerals, the bioavailability of dunaliella salina mineral elements is improved. According to the culture method, a product with the function of current ordinary dunaliella salina and health-care effects can be developed, a new generation of brand-new dunaliella salina product which is rich in specific mineral nutrition and has high bioavailability can be developed, and further the requirements of market refinement are met.

The invention discloses a traditional Chinese medicinal facial mask. The traditional Chinese medicinal facial mask is prepared from the following components as raw materials in parts by weight: 10 to 15 parts of traditional Chinese medicinal liquid, 15 to 20 parts of aloe, 15 to 20 parts of passion fruit, 10 to 20 parts of dunaliella salina, 5 to 10 parts of jasminum grandiflorum, 5 to 10 parts of water lily, 1 to 2 parts of an emulsifier, 1 to 2 parts of a thickening agent and 1 to 2 parts of a moisturizer, wherein the traditional Chinese medicinal liquid is prepared from the following components in parts by weight: 20 to 30 parts of marjoram, 20 to 30 parts of inonotus obliquus, 10 to 20 parts of poria with hostwood, 5 to 10 parts of valerian root, 5 to 10 parts of flos albiziae, 3 to 5 parts of an antioxidant and 0.5 to 1 part of a preservative. In addition, the invention also provides a preparation method of the traditional Chinese medicinal facial mask for treating the insomnia. The traditional Chinese medicinal facial mask disclosed by the invention has the advantages that whitening, moisturizing and fine line removal for facial skin can be effectively realized, sleep is promoted as well as the insomnia is improved; the traditional Chinese medicinal facial mask has a broad application prospect.

A method for using artemia to kill the halophilous algae is sunned pool features that after the running of sunned pool is stabilized, the living artemia area introduced to the gradient layer of sunnel pool, in the next period the bittern in lower convection layer is pumped out and high-concentration fresh bittern is poured in the pool, and before the salt is collected, the artemia and its ova are collected. Its advantages are low investment, and quickly taking its effect.

The invention relates to a light regulated gene expression vector, and particularly, relates to a light regulated gene expression vector suitable for dunaliella salina and an application thereof. The vector includes two gene expression cassettes: 1) a photosensitive transcription factor GAVPO expression cassette including a dunaliella salina actin promoter and a photosensitive transcription factor GAVPO; and 2) a target gene expression cassette regulated by the photosensitive transcription factor GAVPO. The invention further provides the application of the light regulated gene expression vector in expression of a target gene in dunaliella salina through light regulation, dunaliella salina cells transfected with the light regulated gene expression vector can effectively regulate expression of the target gene through blue light, and the expression efficiency is high.

A method for extracting carotinoid and edible glycerin from Dunaliella sallina is provided, which comprises the following procedures: optimized nurture of Dunaliella sallina, concentrate the sallina liquid with hollow fiber film method, centrifugal separation, dehydration and drying, extraction of carbon dioxide with super-critical method. The invention adds no chemical reagent in the extraction process, thus ensuring environment-friendly performance, low loss rate of Dunaliella sallina in the extraction process, high recovery rate of carotinoid and edible glycerin, simple process, low cost and suitable for large scale production.

The invention discloses a method for controlling Dunaliella viridis in a large-area Dunaliella salina culture pond. The method comprises the following steps: 1) sprinkling quicklime into the culture pond, introducing brine with a baume degree of 20 to 24 DEGs into the culture pond to a water depth of 20 to 30 cm and carrying out evaporation to concentrate the brine to a baume degree of 26 to 28 DEGs, wherein salt crystals are precipitated at the bottom of the pond; 2) injecting water to adjusting the salinity of the brine to a baume degree of 20 to 24 DEGs; and 3) inoculating the pond with Dunaliella salina with a certain density, allowing Dunaliella salina to have population advantages, and controlling salinity, the addition amount of nutritional salts and the growth of Dunaliella viridis so as to produce a Dunaliella salina product with a high carotene content, wherein the carotene content finally reaches 8 to 12 mg / L after culture.

The invention relates to composite dunaliella salina and a preparation method thereof, and belongs to the technical field of foods. The composite dunaliella salina is prepared from the following raw materials in parts by weight: 1000g of refined salt, 10g of potassium ferrocyanide, 45g of potassium iodate, 500g of D-sodium erythorbate, 4kg of dunaliella salina powder, 200g of beta carotene, and 55kg of pure water. The composite dunaliella salina and the preparation method have the characteristics of being simple in process, low in cost, and suitable for mass production; the nutritional components of dunaliella salina powder and beta carotene are not damaged; the physicochemical indexes are within the rated range.

The invention discloses a method for harvesting dunaliella salina by using a waste spirulina culture liquid, and relates to a dunaliella salina harvesting method. The method comprises the following steps: 1) collecting supernate after spirulina is harvested, and testing the pH value; 2) adding the supernate collected in the step 1) into dunaliella salina, leaving to stand, and testing the harvesting rate; and 3) mixing the supernate obtained in the step 2) with a dunaliella salina culture medium, inoculating dunaliella salina cells, performing circulation culture, and testing the growth curve of dunaliella salina. By making full use of characteristics that the pH of the culture liquid used in the growth process of spirulina is alkali and dunaliella salina can be automatically flocculated under a high pH value, the supernate of spirulina can be sufficiently utilized, the supernate obtained after dunaliella salina is harvested can be continuously adopted for dunaliella salina culture, and the production cost is low. The harvested dunaliella salina is free of organic test agent or toxic or harmful substance, influence to the quality of an algae paste product in the harvesting process can be avoided, the waste supernate can be sufficiently utilized, and secondary pollution can be prevented.

The invention relates to a method for extracting carotenoid and edible glycerinum from dunaliella salina. The method comprises the following steps: optimally cultivating dunaliella salina; concentrating algae solution according to a hollow fiber membrane method; centrifugally separating; dewatering and drying; and performing supercritical CO2 extraction. In the whole extraction process of the method, no chemical reagent is added, and the environment-friendly effect is achieved; in the extraction process, the fracture loss ratio of dunaliella salina is low and the recovery rate of carotenoid and edible glycerinum is high; the process is simple; the cost is low; the method is suitable for scale production.

The invention relates to a three-dimensional compound cultivation method of sea cucumber seedlings and adult ginseng, which is characterized in that adult ginseng is cultured in a sea cucumber breeding pond, and young ginseng seedlings are cultivated in a suspended seedling cultivation workshop built in the breeding pond. The replacement water with temperature difference in the suspended seedling cultivation workshop flows directly into the multiplication pond, and the inflow ratio is controlled to regulate the water temperature in the multiplication pond in winter ≥ 5 ℃, summer ≤ 24 ℃, the optimum temperature is 10-15 ℃, and the inflow of replacement water brings success Participate in the bait and no longer add other bait. The suspended seedling cultivation workshop regulates the water temperature at 19-23°C, adds baits such as chaetoceros and salina, and renews the water body by flowing water, with a daily flow of 50%-100%. Seedlings are released every spring and autumn in the sea cucumber breeding pond when the daily water temperature is ≥10°C and the daily minimum water temperature is ≥5°C. The water in the pond is changed 4-6 times / month, and the water volume is 30%-50% each time. The method solves the problems of low land utilization rate for sea cucumber cultivation, low water exchange and bait utilization rate of sea cucumber nursery ponds, and the like.

The invention relates to a high-added-value treating method for waste culture liquid of Dunaliella salina. The method comprises the following steps: subjecting waste culture liquid of Dunaliella salina obtained after collection of cells of Dunaliella salina to sand filtration so as to remove insoluble impurities; then carrying out adsorption with negative X-5 resin; and carrying out elution to recover Dunaliella salina polysaccharide and glycerin in the waste culture liquid of Dunaliella salina, supplementing nutrients into a filtrate and then using the filtrate as a Dunaliella salina medium again. Experimental results show that the method recovers 8.59 mg of Dunaliella salina polysaccharide and 2.87 mg of glycerin per L of the waste culture liquid and has recovery efficiency of 11.6 and 7.8%, respectively. The method enables the waste culture liquid of Dunaliella salina to be reused three times and can retain the biomass of Dunaliella salina, the content of carotenoids and the like; the method increases added value, saves the water resource and salt and is easy to implement; and the key points are that the water resource is save, additional benefits are produced, and a good method is provided for development of Dunaliella salina and natural carotenoid resources.