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Method for extracting carotenoid and edible glycerinum from dunaliella salina

A technology of carotene and edible glycerin, which is applied in the separation/purification of hydroxyl compounds, chemical recovery, organic chemistry and other directions, can solve the problems of affecting the recycling of culture solution, strict quality deviation requirements, and high collection cost, and achieve the amount of algae damage. Less, high economic value, environmentally friendly effect

Inactive Publication Date: 2017-01-04
胡培芹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the current concentration and collection methods include high-speed centrifugation, flocculation and precipitation, hydrophobic adsorption, alkaline flocculation and air flotation, etc. Among them, the direct use of high-speed centrifugation technology has high cost, high energy consumption, and strict requirements on quality deviation; flocculation and precipitation The method, hydrophobic adsorption method, and alkaline flocculation and air flotation method all need to add chemical reagents, which not only increases the input, but also affects the recycling of the culture medium.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Under the following main nutrient concentrations, the concentration of sodium bicarbonate is 10mmol / L, the concentration of sodium nitrate is 1mmol / L, the concentration of sodium dihydrogen phosphate is 0.2mmol / L, and the concentration of sodium chloride is 1.5mol / L, culture for 7 days , the biomass is 1 million / ml, the carotenoid content reaches 10 g / m3, and the practical glycerin content reaches 20 g / m3;

[0022] Hollow fiber membrane modules are used to concentrate the above algae liquid. The hollow fiber membrane interception rate is 30KDa. The process adopts cross-flow circulation mode. The circulation pump is a peristaltic pump. The operating pressure is 0.1 bar, the flow rate of algae liquid on the membrane surface is 10m / s, and the membrane flux is 30L. / m 2 h, backwash at 5-hour intervals to maintain the flux, use pH 4.0 acid washing to remove inorganic particles attached to the membrane surface, and sodium hypochlorite to clean the protein attached to the memb...

Embodiment 2

[0025] Under the following main nutrient concentrations, the concentration of sodium bicarbonate is 15mmol / L, the concentration of sodium nitrate is 2mmol / L, the concentration of sodium dihydrogen phosphate is 0.4mmol / L, and the concentration of sodium chloride is 1.5mol / L. Culture for 10 days , with a biomass of 1.5 million / ml, of which the content of carotenoids reaches 20 g / m3, and the content of practical glycerin reaches 30 g / m3;

[0026] The hollow fiber membrane module is used to concentrate the above algae liquid. The hollow fiber membrane interception rate is 5KDa. The process adopts the cross-flow circulation mode. 10L / m 2 h, 1-hour interval backwashing to maintain the flux, 1-day intervals with pH 4.0 acid washing to remove inorganic particles attached to the membrane surface, and sodium hypochlorite to clean the protein attached to the membrane surface, and the concentration factor is 30 times. Use a centrifuge to further concentrate the algae liquid, remove the s...

Embodiment 3

[0029] Under the following main nutrient salt concentrations, the concentration of sodium bicarbonate is 12mmol / L, the concentration of sodium nitrate is 1.4mmol / L, the concentration of sodium dihydrogen phosphate is 0.25mmol / L, and the concentration of sodium chloride is 1.5mol / L. day, the biomass is 1.2 million / ml, the carotenoid content reaches 15 g / m3, and the practical glycerin content reaches 23 g / m3;

[0030] Hollow fiber membrane modules are used to concentrate the above algae liquid. The interception rate of the hollow fiber membrane is 15KDa. The process adopts the cross-flow circulation mode. The circulation pump is a peristaltic pump, the operating pressure is 0.2bar, the flow rate of the algae liquid on the membrane surface is 10m / s, and the membrane flux is 14L / m 2h, backwash at 2-hour intervals to maintain the flux, use pH 4.0 acid washing to remove inorganic particles attached to the membrane surface, and sodium hypochlorite to clean the protein attached to th...

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PUM

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Abstract

The invention relates to a method for extracting carotenoid and edible glycerinum from dunaliella salina. The method comprises the following steps: optimally cultivating dunaliella salina; concentrating algae solution according to a hollow fiber membrane method; centrifugally separating; dewatering and drying; and performing supercritical CO2 extraction. In the whole extraction process of the method, no chemical reagent is added, and the environment-friendly effect is achieved; in the extraction process, the fracture loss ratio of dunaliella salina is low and the recovery rate of carotenoid and edible glycerinum is high; the process is simple; the cost is low; the method is suitable for scale production.

Description

technical field [0001] The invention relates to a method for extracting carotenoids and edible glycerin from salina, in particular to a method for extracting carotenoids and edible glycerin from salina. Background technique [0002] Dunaliella Salina is a halophilic planktonic single-celled algae with bare protoplasts. Under the conditions of high salinity, strong light and nitrogen deficiency, the cells can accumulate a large amount of carotene, which accounts for up to 14% of the dry weight of the algal body. Is the main source of natural carotene. Among them, β-carotene is not only the precursor of vitamin A and the natural pigment of food processing, but also has a strong ability to resist oxidation and eliminate oxygen free radicals, and has anti-cancer and anti-cancer effects. It has been widely used in food, hygiene and the field of medicine. At present, the annual demand for β-carotene in the world is 1200-1500 tons, and it is increasing at a rate of 7-9% every yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24C07C31/22C07C29/76C07C29/78
CPCY02P20/582
Inventor 胡培芹
Owner 胡培芹
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