Dunaliella culture method

Abstract

The invention belongs to the technical field of biological culture of algae, and relates to a dunaliella culture method. The dunaliella culture method comprises the following steps: at first, regulating the light intensity and temperature in a dunaliella culture reactor to adapt to dunaliella propagation, inoculating dunaliella into a dunaliella culture agent containing a salt, a nitrogen source (KNO3), a phosphorus source (KH2PO4) and an inorganic carbon source (NaHCO4), respectively measuring the nitrogen source, the phosphorus source and the inorganic carbon source in the dunaliella culture agent after a growth period, a logarithm growth period and a platform period of dunaliella culture, making the nitrogen source, the phosphorus source and the inorganic carbon source respectively reach set values, and measuring dunaliella biomass to complete the dunaliella propagation; regulating the light intensity and the temperature to adapt to beta-carotene accumulation, respectively adding table salt, KNO3, KH2PO4 and NaHCO4 into the dunaliella culture agent to set values, culturing until the dunaliella becomes brown, and then the beta-carotene is accumulated and the dunaliella culture is completed. The dunaliella culture method is scientific and reasonable in culture process, simple in steps, stable in performance, free of external environment influence and high in product utilization rate.

Description

Technical field: [0001] The invention belongs to the technical field of algal biological cultivation, and relates to a method for cultivating salina, which can increase the propagation speed of salina, increase the biomass and promote the accumulation of beta-carotene at the same time, and the cultivated salina can be used in the technical fields of medical care and sanitation and epidemic prevention . Background technique: [0002] Salina is a single-celled eukaryotic algae that lives in high-concentration salt lakes. It belongs to the genus Salina in the family Chlorophyta Chlorophyceae Volvoxaceae. It is a green single-celled algae with a small body, no cell wall, and sugar. The envelope formed by protein is the only strange life found so far that can survive in high-concentration salt water. Because it is rich in unique and rich life elements, it is praised as "the power source of cells" and "the protection agent of life" by the world's scientific community. Salina is r...

AI Technical Summary

Problems solved by technology

[0002] Salina is a single-celled eukaryotic algae that lives in high-concentration salt lakes. It belongs to the genus Salina in the family Chlorophyta Chlorophyceae Volvoxaceae. It is a green single-celled algae with a small body, no cell wall, and sugar. The envelope formed by protein is the only strange life found so far that can survive in high-concentration salt water. Because it is rich in unique and rich life elements, it is praised as "the power source of cells" and "the protection agent of life" by the world's scientific community. Salina is rich in β-carotene, which can be artificially cultivated to extract glycerol or carotene, and can also be used as bait for larvae such as fish, shrimp, and shellfish; it can prevent and assist in the treatment of coronary heart disease, cardiovascular and cerebrovascular diseases in the field of medical and health , diabetes, cancer, liver disease, common stomach diseases, oral ulcers, cataracts, dry eye syndrome, reduce adverse reactions to chemotherapy, improve vision, improve immunity, anti-oxidation, delay aging; because salina has no cell wall, it can be added to animal feed Among them, it can promote the feed conversion rate, increase the growth rate of animals, and reduce diseases. The development of "green feed" is of great significance to improve the quality of animal feed products; the huge economic value and unique advantages of salina have made it the object of large-scale cultivation , Many countries and regions are cultivating and developing salina, including Australia, Japan, Israel, the United States, etc. Hainan and Inner Mongolia in China have also cultivated salina. The current cultivation method uses low-intensity light and temperature, which is suitable for salina The rapid growth of biomass is not conducive to the accumulation of β-carotene, and the relative lack of nutrients, especially nitrogen, reduces the nutritional value; the use of high-intensity light and temperature is conducive to the accumulation of β-carotene, but serious Affected the growth and reproduction of salina