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Method for extracting chlorophyll from dunaliella by using inorganic alkali

A technology of chlorophyll and inorganic alkali, applied in the field of food science, can solve problems such as impracticality and troublesome functional substances, and achieve the effects of saving time, short extraction cycle and high purity

Inactive Publication Date: 2011-10-05
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method will cause trouble for other functional substances, because chlorophyll is generally used in the production of functional foods, and organic solvent residues in foods are not allowed
It has been determined that organic solvents such as acetone and petroleum ether cannot be completely removed whether they are in the extracted chlorophyll or in the subsequent extraction of functional substances such as salina polysaccharides. Therefore, this method has great limitations. This method can be applied in small-scale trials, but in large-scale production, especially in the production of food, it is not feasible to use this method

Method used

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  • Method for extracting chlorophyll from dunaliella by using inorganic alkali

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Effect test

Embodiment 1

[0030] (1) Cool the fresh salina mud to -10°C, freeze for 24 hours, and then thaw in a 30°C water bath until there are no ice crystals;

[0031] (2) adjust pH=11 with sodium hydroxide aqueous solution, extract 3 hours at 60 ℃;

[0032] (3) take the supernatant after centrifuging the extract at 3000r / min for 12min;

[0033] (4) adjusting the supernatant with hydrochloric acid to make pH=4 to precipitate chlorophyll;

[0034] (5) Centrifuge at 3000 r / min for 10 min, remove the supernatant, obtain chlorophyll, and freeze-dry the chlorophyll.

Embodiment 2

[0036] (1) Cool the fresh salt algae mud to -5°C, freeze for 26 hours, and then thaw in a 32°C water bath until there are no ice crystals;

[0037] (2) adjust pH=10 with potassium hydroxide aqueous solution, at 65 ℃, extract 4 hours;

[0038] (3) the extract is centrifuged at 2500r / min for 10min and the supernatant is taken;

[0039] (4) adjusting the supernatant with hydrochloric acid to make pH=4 to precipitate chlorophyll;

[0040] (5) Centrifuge at 2500 r / min for 11 min, remove the supernatant, obtain chlorophyll, and freeze-dry the chlorophyll.

Embodiment 3

[0042] (1) Stir the salina powder with an appropriate amount of water, cool down to -40°C, freeze for 20 hours, and then thaw in a 25°C water bath until there are no ice crystals;

[0043] (2) adjust pH=11 with sodium carbonate aqueous solution, at 60 ℃, extract 5 hours;

[0044] (3) take the supernatant after centrifuging the extract at 3500r / min for 8min;

[0045] (4) adjusting the supernatant with hydrochloric acid to make pH=4.5 to precipitate chlorophyll;

[0046] (5) Centrifuge at 3500 r / min for 9 min, remove the supernatant, obtain chlorophyll, and freeze-dry the chlorophyll.

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Abstract

The invention discloses a method for extracting chlorophyll from dunaliella by using inorganic alkali, which comprises the following steps: (1) cooling and freezing dunaliella mud or dunaliella powder added with a proper amount of water, unfreezing in a water bath till ice crystals are eliminated; (2) regulating pH value by using aqueous solution of an inorganic alkali, and extracting for 3 to 5 hours at 60 to 70 DEG C; (3) centrifuging the extract solution and collecting supernate; (4) regulating the pH value of the supernate by using an inorganic acid to precipitate chlorophyll; and (5) centrifuging, removing supernate to obtain chlorophyll and freezing and drying the chlorophyll. The method disclosed by the invention overcomes the drawback of organic solvent residue of the product obtained by an organic solvent extraction method and the drawbacks of big investment and high economic cost of a supercritical extraction. When the method is used, a convenience is provided for chlorophyll extraction and the trouble brought by the need of removing acetone, petroleum ether and other organic solvents in a subsequent polysaccharide extraction process is avoided.

Description

technical field [0001] The invention belongs to the field of food science and technology. Specifically, it relates to a method for extracting chlorophyll in Salina salina with inorganic alkali. Background technique [0002] Salina salina is a single-celled eukaryotic alga and one of the most salt-tolerant eukaryotes discovered so far. It belongs to high-salt stress organisms, with strong adaptability and rapid reproduction. Its natural living environment is mostly salt ponds and salt lakes. In other places, the special environment has created a unique growth mechanism. Dunaliella salina is a photoautotrophic organism, rich in oil, carotene, chlorophyll, protein, polysaccharide, etc., and high in Ca, P, Zn and other minerals, and also contains 18 kinds of amino acids including human essential amino acids , the accumulated glycerol is 40% to 50% of the dry weight. The content of inorganic elements in salina is high, and the content ratio of each element is appropriate, and ...

Claims

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Application Information

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IPC IPC(8): C07D487/22C09B61/00
CPCY02P20/54
Inventor 刘安军郑捷王雅静王维君
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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