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Culture method of dunalilla salina

A technology of salt algae and culture medium, which is applied in the field of cultivation of salina salina, which can solve the problems of insignificant increase in the biomass and metabolites of salina salina, high viscosity of the culture solution, and unfavorable algae absorption, etc., and achieves easy large-scale cultivation and simple operation , the effect of promoting the growth and division of algae cells

Inactive Publication Date: 2007-03-07
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

It has been found through research that the amount of exopolysaccharide secreted by algae is high, so that the viscosity of the culture medium is relatively large, which is not conducive to the absorption of nutrients by algae, resulting in a certain degree of inhibition of growth (Su Chuandong. Salinity and nutrient restrictions on Cyanobacteria (Cyanothec.sp) ​​113 strain growth and the effect of exopolysaccharide production, Marine Limnology Bulletin, 2005, 4: 69-73)
None of the above culture methods can solve the problem of polysaccharides inhibiting the growth of algae cells during the cultivation of Salina salina, so the effect of improving the biomass of Salina salina and the accumulation of metabolites (especially β-carotene) is not significant

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  • Culture method of dunalilla salina
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  • Culture method of dunalilla salina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the cultivation of salina and the influence of inoculated bacillus of different densities on the growth rate of salina 5The density of cfu / mL was inoculated in Modified Johnson's medium (pH 7.5), and at the same time, according to the inoculation amount of 0 (control), 10, 100, and 1000 cfu / mL, Bacillus pumilus was inoculated into the medium, and at 24 ℃, light intensity 150μmoles·m -2 ·s -1 , under the condition of light time 10h / day, culture Salina salina to detect the influence of different densities of Bacillus on the growth rate of Salina salina. When culturing to the end of logarithmic growth, count with a hemocytometer under a microscope the next day, using the formula K=(N-N 0 ) / T(N is the number of algal cells per unit volume after T time cultivation, N 0 The initial unit volume of algae cells is cultivated for T time, and T is the culture time (day)) to calculate the daily average growth rate (K) of salina. The statistical results of the cell...

Embodiment 2

[0020] The cultivation of embodiment 2, salina and the influence of the bacillus of inoculating different densities on the biomass of salina

[0021] Press Salina to 1×10 5 The density of cfu / mL was inoculated in Modified Johnson's medium (pH 7.5), and at the same time, Bacillus subtilis (Bacillus subtilis) was inoculated into the medium according to the inoculation amount of 0 (control), 10, 100, and 1000 cfu / mL respectively. ℃, light intensity 100μmoles·m -2 ·s -1 , light time 14h / day, under the conditions of 100rpm, shake culture of Salina salina, in order to detect the impact of different densities of Bacillus on the biomass of Salina salina. When the culture reaches the end of logarithmic growth, take 400mL of the algae solution inoculated with different densities of Bacillus, centrifuge at 4800rpm for 10min, discard the supernatant, desalinate the algae, freeze, vacuum dry, and weigh to obtain the biomass of Salina. The statistical results of biomass are shown in Figu...

Embodiment 3

[0022] Example 3, the cultivation of salina and the impact of inserting different densities of bacillus on the accumulation of salina β-carotene

[0023] Press Salina to 1×10 5 The density of cfu / mL was inoculated in Modified Johnson's medium (pH 7.5), and at the same time, Bacillus coagulans (Bacillus Coagulans) was inoculated into the medium according to the inoculation amount of 0 (control), 10, 100, and 1000 cfu / mL respectively, at 25 ℃, light intensity 130μmoles·m -2 ·s -1 , light time 12h / day, 130rpm under the conditions of shaking culture of Salina salina, in order to detect the impact of inoculating different densities of Bacillus on the accumulation of β-carotene in Salina salina. During the cultivation process, the β-carotene accumulation of Salina salina was measured every 3 days. The method is as follows: First, according to the literature (Liu Jianguo, Wu Chaoyuan. Review of Salina salina and β-carotene research [J]. Ocean and Limnology, 1995, 26 (3): The metho...

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Abstract

The invention discloses a culturing method of salt algae, which is characterized by the following: seeding bacillus in the culture medium; obtaining the growing speed at 4.135*105 / d and biological quantity at 846.88mg .L-1 and accumulating quantity of beta-carotene at 125.4mg .L-1; accelerating the development of salt algae and beta-carotene industry.

Description

technical field [0001] The invention relates to a method for cultivating algal plants, in particular to a method for cultivating salina. Background technique [0002] Salina is an algal species that was cultivated earlier in China and has relatively mature cultivation methods. It has the characteristics of fast growth and large accumulation of beneficial metabolites. Salina can accumulate a large amount of β-carotene in a stress environment (high temperature, high salt and / or strong light), up to 14% of the dry weight of algal cells, and is one of the organisms with the highest β-carotene content in nature. Studies have shown that β-carotene is not only a precursor of vitamin A and a natural pigment in the food processing industry, but also prevents cancer, tumors and cardiovascular diseases, as well as age-related degenerative diseases such as senile dementia and cataracts; When the medium is used to cultivate Salina salina, it can also accumulate a large amount of protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P23/00
Inventor 郑维发杜彩华陈才法储成才
Owner XUZHOU NORMAL UNIVERSITY
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