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Method for controlling Dunaliella viridis in large-area Dunaliella salina culture pond

A culture pond, large-area technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., to achieve the effect of improving breeding efficiency and product quality

Inactive Publication Date: 2017-06-30
JINGHE COUNTY JINGHE SALINIZATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disinfection treatment is often used to kill Salina Dunaliella viridis in small-area racetrack ponds, but it is difficult to use this method in large-area ponds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for controlling salina salina in a large-area cultivation pond, comprising the steps of:

[0015] 1) Sprinkle 20kg of quicklime in proportion to each mu of culture pond, introduce 22°B brine into the culture pond to a water depth of 25cm, utilize natural evaporation to concentrate the brine to 27°B, and there are salt crystals at the bottom of the pond to separate out;

[0016] 2) Pour in water to adjust the brine salinity to 22°Bé;

[0017] 3) Add 1 / 3 part of the total amount of nutrient salts, insert Dunaliella salina until the algae density is 30,000 cells / ml, detect the algae density every day, and wait until Dunaliella salina grows to 70,000 cells / ml, and miscellaneous When the proportion of the number of Dunaliella viridis is less than 10% of the total number of Salina, add 1 / 3 part of the total amount of nutrient salt for the second time; when Dunaliella salina grows to 130,000 cells / ml, and the number of Dunaliella viridis When the ratio is less than 2...

Embodiment 2

[0020] A method for controlling salina salina in a large-area cultivation pond, comprising the steps of:

[0021] 1) Sprinkle 25kg of quicklime in proportion to each mu of culture pond, introduce 20°B brine into the culture pond to a depth of 20cm, utilize natural evaporation to concentrate the brine to 26°B, and there are salt crystals at the bottom of the pond to separate out;

[0022] 2) Pour in water to adjust the brine salinity to 24°Bé;

[0023] 3) Add 1 / 3 part of the total amount of nutrient salts, insert Dunaliella salina until the algae density is 20,000 cells / ml, detect the algae density every day, and wait until the Dunaliella salina grows to 50,000 cells / ml, and the miscellaneous When the proportion of the number of Dunaliella viridis is less than 10% of the total number of Salina, add 1 / 3 part of the total amount of nutrient salt for the second time; when Dunaliella salina grows to 90,000 cells / ml, and the number of Dunaliella viridis When the ratio is less than ...

Embodiment 3

[0026] A method for controlling salina salina in a large-area cultivation pond, comprising the steps of:

[0027] 1) Sprinkle 15kg of quicklime in proportion to each mu of culture pond, introduce 20°B brine into the culture pond to a water depth of 30cm, utilize natural evaporation to concentrate the brine to 28°B, and there are salt crystals at the bottom of the pond to separate out;

[0028] 2) Pour in water to adjust the brine salinity to 20°Bé;

[0029] 3) Add 1 / 3 part of the total amount of nutrient salts, insert Dunaliella salina until the algae density is 40,000 cells / ml, detect the algae density every day, and wait until the Dunaliella salina grows to 80,000 cells / ml, and miscellaneous When the proportion of the number of Dunaliella viridis is less than 10% of the total number of Salina, add 1 / 3 part of the total amount of nutrient salt for the second time; when Dunaliella salina grows to 160,000 cells / ml, and the number of Dunaliella viridis When the ratio is less th...

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Abstract

The invention discloses a method for controlling Dunaliella viridis in a large-area Dunaliella salina culture pond. The method comprises the following steps: 1) sprinkling quicklime into the culture pond, introducing brine with a baume degree of 20 to 24 DEGs into the culture pond to a water depth of 20 to 30 cm and carrying out evaporation to concentrate the brine to a baume degree of 26 to 28 DEGs, wherein salt crystals are precipitated at the bottom of the pond; 2) injecting water to adjusting the salinity of the brine to a baume degree of 20 to 24 DEGs; and 3) inoculating the pond with Dunaliella salina with a certain density, allowing Dunaliella salina to have population advantages, and controlling salinity, the addition amount of nutritional salts and the growth of Dunaliella viridis so as to produce a Dunaliella salina product with a high carotene content, wherein the carotene content finally reaches 8 to 12 mg / L after culture.

Description

technical field [0001] The invention belongs to a method for controlling the salina Dunaliella salina (we call the salina salina dunaliella viridis) in large-area culture ponds. Background technique [0002] Salina Dunaliella salina is a diflagellated unicellular microalgae. Its light energy conversion rate is high, and its growth and reproduction are fast. The suitable salinity range for its growth is 6% to saturated brine, and it can quickly accumulate carotene under high salinity. It is the biological species with the highest carotene content in nature. In addition to natural carotene, salina is also rich in polysaccharides, diterpenes and other biologically active substances and minerals needed by the human body. These substances have unique chemical structures, and their functions are irreplaceable by land organisms. For economical algae. [0003] When large-scale cultivation of salina is carried out, there are usually two kinds of salina in the water body: Dunaliella...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 辛乃宏杨俊涛李炳乾王新磊赵喜荣刘颖芬
Owner JINGHE COUNTY JINGHE SALINIZATION
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