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Dunaliella salina culture medium

A culture medium formula, salina technology, applied in microorganism-based methods, single-cell algae, biochemical equipment and methods, etc., can solve the problems of low cell density, unreasonable design, slow growth rate of algal cells, etc.

Active Publication Date: 2015-04-29
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the unreasonable design of the salt algae culture medium currently used, the algae cell growth rate is slow and the cell density is low, so it is not suitable for large-scale industrial production

Method used

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  • Dunaliella salina culture medium
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) configure the seawater medium of the following formula:

[0014] NaCl 5g / L, NaNO 3 0.5g / L, KH 2 PO 4 45mg / L, ferric citrate 1mg / L, potassium acetate 0.2g / L, glycine 0.25g / L, production-increasing amine DCPTA 1mg / L, ZnSO4 4H2O 23μg / L, MnCl2 4H2O 178μg / L, CuSO4 5H2O 10μg / L , Na2MoO4·2H2O 7.3μg / L, CoCl2·6H2O 12μg / L.

[0015] (2) Take Dunaliella salina in the logarithmic growth phase and inoculate it into the medium for cultivation, light for 10 hours a day, the light intensity is about 50 μmol / (m2 s), the temperature of the incubator is controlled at about 25 degrees Celsius, and every other period Time records the OD700 of Chromosalis in the culture medium.

Embodiment 2

[0017] (1) Configure seawater culture medium with the following formula

[0018] NaCl 10g / L, NaNO 3 0.5g / L, KH 2 PO 4 100mg / L, ferric citrate 1mg / L, potassium acetate 0.3g / L, glycine 0.4g / L, amine DCPTA 10mg / L, ZnSO 4 4H 2 O 23μg / L, MnCl 2 4H 2 O 178μg / L, CuSO 4 ·5H 2 O10μg / L, Na 2 MoO 4 2H 2 O 7.3 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

[0019] (2) Take the Dunaliella salina in the logarithmic growth phase and inoculate it into the culture medium, light it for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator is controlled at about 25 degrees Celsius, and the OD700 of Chromosalis in the medium is recorded at regular intervals.

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Abstract

The invention discloses a dunaliella salina culture medium. The dunaliella salina culture medium is prepared from 5-10 g / L of NaCl, 0.5 g / L of NaNO3, 45-100 mg / L of KH2PO4, 1 mg / L of ferric citrate, 0.2-0.3 g / L of potassium acetate, 0.25-0.4 g / L of glycine, 1-10 mg / L of dichlorophenoxy triethyl-amine DCPTA, 23 mu g / L of ZnSO4.4H2O, 178 mu g / L of MnCl2.4H2O, 10 mu g / L of CuSO4.5H2O, 7.3 mu g / L of Na2MoO4.2H2O, and 12 mu g / L of CoCl2.6H2O. The culture medium prepared according to the invention has the characteristics of high growth speed, large cell density, and the like in the process of culturing dunaliella salina.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a culture medium of salina. Background technique [0002] Salina belongs to Chlorophyta, Chlorophyceae, Volvocales, Dunaliellaceae, and Salina genus in classification. The main culture species is Dunliella.Salina. Dunaliella, also known as Dunaliella, is a single-celled marine planktonic economic algae that is widely distributed in oceans, salt ponds, brackish lakes and brackish water all over the world. It is the only known eukaryote that can grow in close to fresh water to saturated saline solutions . [0003] Because of its small size, fast reproduction and rich nutrition, it is a high-quality bait for rare marine aquatic animals such as sea cucumbers, abalones, and shrimps. With the rapid development of aquaculture industry, the market demand for unicellular algae bait is also increasing. On the other hand, Salina cells are rich in β-carotene. Under certain growth conditions, the ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟彭小伟张君纲王弢张云王琦靳立兵
Owner NINGBO FUTIAN BIOTECH
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