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Culture method for improving contents and bioavailability of mineral elements in dunaliella salina cells

A mineral element and culture method technology, which is applied in the field of selective cultivation of mineral element nutrition and bioavailability in salina cells, can solve the problems of not being able to meet the needs of mineral nutrition and its content, and achieve improved Bioavailability, effect of increasing content

Inactive Publication Date: 2017-09-01
TIANJIN ACELBIO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the refinement of products in the market, especially the requirements for specific mineral nutrition, the use of natural conditions for large-scale farming or wild free-range cultivation of Salina is limited by natural and artificial breeding conditions, and can no longer meet the market's requirements for specific minerals. Nutrients and their content requirements

Method used

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  • Culture method for improving contents and bioavailability of mineral elements in dunaliella salina cells
  • Culture method for improving contents and bioavailability of mineral elements in dunaliella salina cells
  • Culture method for improving contents and bioavailability of mineral elements in dunaliella salina cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A culture method for improving mineral elements and their bioavailability in salina cells:

[0023] (1) Medium preparation: The improved f / 2 medium is used, in which each liter of medium contains 20.785mg NaCl, 3.477mg NaCl 2 SO 4 , 0.587mg KCl, 0.17mg NaHCO 3 , 0.0845mg KBr, 0.0225mg H 3 BO 3 , 0.0027mgNaF, 9.395mg MgCl 2 ▪6H 2 O, 1.316mg CaCl 2 ▪2H 2 O, 0.0214mg SrCl2 ▪6H 2 O, 75mg NaNO 3 , 5.0mgNaH 2 PO 4 ▪H 2 0. 4.36 mg Na 2 EDTA, 3.16mg FeCl 3 ▪6H 2 O, 0.01mg CuSO 4 ▪5H 2 O, 0.023mg ZnSO 4 ▪7H 2 O, 0.012mg CoCl 2 ▪6H 2 O, 0.018mg MnCl 2 ▪4H 2 O, 0.07mg Na 2 MoO 4 ▪2H 2 O, 0.012mg Na 2 SeO 3 , 0.0001mg Vitamin B 1 , 0.0005mg Vitamin B 12 and 0.0005mg biotin;

[0024] (2) Salina inoculation and cultivation: the initial density of salina in the culture medium is 1×10 4 ~1×10 5 Cells / mL, increase the ion concentration of minerals in the culture medium at different stages of Salina salina culture 1-14 days, culture temperature is 25±1℃, ...

Embodiment 2

[0027] (1) Use the improved f / 2 medium for salina culture, the culture temperature is 25±1°C, the light intensity is 3500lux, the light-to-dark ratio is 12h / 12h, the growth cycle is 15 days, and it starts to enter on the 10th day In the logarithmic growth stage, the growth curve of salina is shown in the appendix figure 1 .

[0028] After 15 days of cultivation of Salina salina, the salina salina was collected and vacuum-dried at 40°C. The contents of five biomineral elements, calcium, magnesium, iron, zinc and selenium in the salina cells were determined by atomic absorption analysis. The results As in Table 1:

[0029] Table 1 Contents of minerals in salina

[0030]

[0031] (2) The improved f / 2 medium was used as the basic medium for salina culture, and CaCl was added to the basic medium for salina 2 ▪2H 2 O and MgCl 2 ▪6H 2 The content of O makes the ion concentrations of calcium and magnesium in the basic culture medium of Salina salina to be 7.066mg / L and 22.215...

Embodiment 3

[0065] Comparative Test:

[0066] (1) Use the improved f / 2 medium as the basic medium for salina culture, and add CaCl to the basic medium for salina 2 and MgCl 2 , so that the Ca in Salina basal medium 2+ and Mg 2+ The concentration increased 20 times, while without adding CaCl 2 and MgCl 2 Salina salina cultured in the basic medium was used as a control. After 15 days of culture, the salina salina was collected and vacuum-dried at 40°C. The contents of mineral calcium and magnesium in the cells of two kinds of salina, the results are shown in Table 2:

[0067] Table 2 Determination of calcium and magnesium content in salina cells

[0068]

[0069] The results show that increasing the concentration of calcium and magnesium ions in the medium can increase the content of mineral calcium and magnesium in the cells of Salina salina. times, the mineral magnesium content increased by 3.62 times.

[0070] (2) The bioavailability of calcium and magnesium elements in vivo w...

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Abstract

The invention discloses a culture method for improving the contents and the bioavailability of mineral elements in dunaliella salina cells. The culture method is mainly characterized in that ion concentrations of specific inorganic mineral elements of calcium, magnesium, iron, zinc and selenium are selectively added in an existing dunaliella salina culture medium, so that the contents of specific biological mineral elements in the dunaliella salina cells can be increased and compositions of different mineral elements can be adjusted; meanwhile, by transforming inorganic mineral nutrition into biological minerals, the bioavailability of dunaliella salina mineral elements is improved. According to the culture method, a product with the function of current ordinary dunaliella salina and health-care effects can be developed, a new generation of brand-new dunaliella salina product which is rich in specific mineral nutrition and has high bioavailability can be developed, and further the requirements of market refinement are met.

Description

[0001] This application is supported by Tianjin Key Laboratory of Medicinal Plant Cell Scale Cultivation Enterprise. technical field [0002] The invention relates to a cultivation method of algae, in particular to a cultivation method for selectively improving the nutrition and bioavailability of mineral elements in cells of salina. Background technique [0003] Salina is a single-celled eukaryotic algae of great economic value, mainly distributed in salt water bodies such as oceans and salt lakes, and is the most salt-tolerant eukaryotic photosynthetic organism found so far. Salina is rich in a variety of natural carotenoids, vitamins, dietary fiber, amino acids and various mineral elements required for human health, and has biological activities such as improving immunity, anti-oxidation, and delaying aging. At present, many countries, including the United States, Israel, Australia, etc., have successively carried out the development of salina health products, and have en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 宋静静张卫刘永彬翟丽娟谭光伟
Owner TIANJIN ACELBIO BIOTECH
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